Team:Manchester/Improve
Improved Parts
Please visit our Part Collection page for ALL PARTS.
The Manchester iGEM 2017 team have improved part BBa_K562001, originally submitted by team Dundee 2011.
Improvement over old part:
We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.
We have also further improved this part by adding different anderson promoters (BB LInk bb link...) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.
| Part Name | BioBrick | Description | Part Type |
|---|---|---|---|
| LowPromoter_PduD(1-20)_mCherry | BBa_K2213006 | Part Description | Basic |
| MediumPromoter_PduD(1-20)_mCherry | BBa_K2213007 | Part Description. | Basic |
| HighPromoter_PduD(1-20)_mCherry | BBa_K2213008 | Part Description. | Basic |
