Team:Lethbridge

Cell-free Synthetic Biology for the Masses



Why Cell-free?


Cell-free systems include all the necessary biomachinery for producing proteins outside of a living cell

Due to their inherent safety and simplicity, cell-free systems provide a powerful tool to synthetic biologists for engineering novel systems without the constraints of cellular life


Motivation

A lack of standardization, simplification and flexibility has limited the application of cell-free systems, including its use within the education system and DIY community

We have identified three target user-groups for our system:



Design

To lower the barrier of synthetic biology our project aims to create a STANDARD, SAFE, MODULAR and USER-FRIENDLY cell-free system



Our System


A collection of standard parts for essential TX-TL components


Streamline and novel purification techniques





A tool to create an orthogonal system





A tool to detect potentially harmful encrypted sequences






A safe synthetic biology teaching tool
Simplified protocols
Curriculum aligned lesson plans