Team:AFCM-Egypt/Part Collection

Parts

We submitted the basic parts constituting our gene fragments, then
we designed the composite parts constituting these synthesised fragments
and finally we assembled these composite parts into our two main circuits
designed in order to compare their function at regulating non-coding RNAs.

Basic Parts

Part Name	Type	Description	Length	Link
BBa_K2217000	Basic	CMV Enhancer	304	HERE
BBa_K2217001	Basic	Circular RNA hsa-circ-000064	139	HERE
BBa_K2217002	Basic	U6 Promoter for Cas9 gRNA	204	HERE
BBa_K2217003	Basic	gRNA for Cas9 Knock in	20	HERE
BBa_K2217004	Basic	gRNA scaffold for Cas9	76	HERE
BBa_K2217005	Basic	SV40 Terminator	122	HERE
BBa_K2217006	Basic	CMV Promoter	204	HERE
BBa_K2217007	Basic	T7 Promoter	19	HERE
BBa_K2217008	Basic	CYC1 Terminator	250	HERE
BBa_K2217009	Basic	HDR Template of BBa_K2217001	139	HERE
BBa_K2217010	Basic	Chicken B-actin Promoter	278	HERE
BBa_K2217012	Basic	laci	1080	HERE
BBa_K2217014	Basic	YFP	720	HERE
BBa_K2217015	Basic	bGH poly(A) signal	225	HERE
BBa_K2217016	Basic	lac_operator	17	HERE
BBa_K2217017	Basic	lac_promoter	31	HERE
BBa_K2217021	Basic	miRNA-1825 binding site on hsa-circ-00064	20	HERE

Part Name	Description	Length	Link
BBa_K2217019	Cas9 Knock-in	6035	HERE
BBa_K2217020	BBa_K2217001 ceRNA hsa-circ-0000064	867	HERE
BBa_K2217022	laci + miRNA-1825 binding site on hsa-circ-0000064 Production	1732d	HERE

Composite Parts representing Synthesized
Fragments

These composite parts were synthesised as separate gene fragments then assembled to form BBa_K2217025 and BBa_K2217026 representing ceRNA and CRISPR circuits respectively.

Part Name	Description	Length	Link
BBa_K2217024	CMV Enhancer + CMV Promoter + T7	542	HERE
BBa_K2217027	laci + miRNA-1825 binding site synthesized fragment	1210	HERE
BBa_K2217028	BBa_K2217001 ceRNA hsa-circ-0000064 + sv_poly(A) Synthesized Fragment	269	HERE
BBa_K2217013	CAG Promoter	590	HERE
BBa_K2217023	YFP for ceRNA circuit characterization	1015	HERE
BBa_K2217018	gRNA for Cas9 Knock-in	483	HERE
BBa_K2217029	CMV Enhancer + CMV Promoter	516	HERE