Team:Lethbridge/Design
Design Objectives
Our design was guided by the needs of three user groups:
Simple scientific protocols
Standardized parts
Well characterized and developed system
Standard, easy to use system
Simplified protocols
Modular tool that can be tailored to the application
Modular experiments
Open-source
Open-source
Promote good safety practices
Standardized parts
Resulting in the identification of four core design considerations for our system
STANDARD, MODULAR, SAFE, and USER-FRIENLDY
Overview
To ensure utility of our system, we developed a simplified expression and purification strategy to produce the necessary biomachinery for cell-free synthetic biology.
Standard Construct Design
Purification Strategy
tRNA
Ribosomes
Validation Construct
Next vivo 2.0
