Team:MIT/protocols

• ABOUT US
  Team Attributions
• BACKGROUND
  Alternative Splicing CRISPR RNA Binding Proteins REST
• PROJECT
  Guides and ASOs mKate-FF4 2 Exon mKate HBG 3 Exon mKate HBG 3 Exon Dual Fluorescence
• LAB WORK
  Parts/Improved Parts Protocols Notebook Future Work
• MODELLING
• HUMAN PRACTICES
  Integrated HP Public Engagement Collaborations InterLab
• AWARDS
  HP Silver HP Gold Education & Public Engagement Model Parts Collection

Protocols

Bacterial Lab

Transformation of E. coli
Sequencing
Sample Preparation for Flow Cytometry
Resuspending Primers
Resuspending_gBlocks
Primer Design
Pouring LB Agar Plates
PCR
Overnight liquid cultures
Nanodrop
Miniprep
LR Reaction
Golden Gate Assembly
Glycerol Stock
Gel Extraction
Gel Extraction
Diagnostic Restriction Digestion
Annealing Oligonucleotides
Pouring an Agarose Gel

Tissue Culture

Culturing & Splitting HEK-293 Cells
Culturing & Splitting NCI-H82 Cells
DNA Transfection into HEK Cells
Seeding Plates