Team:Heidelberg/Materials
Notebook
Materials
Antibiotics
| Name | Supplier | |
|---|---|---|
| Carbenicillin dissodium salt | Carl Roth | |
| Amicillin sodium salt | Carl Roth | |
| Chloramphenicol | Carl Roth | |
| Kanamycin sulfate | Sigma Aldrich | |
| Tetracycline | Sigma Aldrich | |
| Spectinomycine | Sigma Aldrich |
Enzymes
| Name | Supplier | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| BamI | New England BioLabs | |||||||||
| BamHI-HF | New England BioLabs | |||||||||
| BgIII | New England BioLabs | |||||||||
| BsaI-HF | New England BioLabs | |||||||||
| BsmBI | New England BioLabs | |||||||||
| DpnI | New England BioLabs | |||||||||
| EcoRI | New England BioLabs | |||||||||
| EcoRI-HF | New England BioLabs | |||||||||
| Esp3I | Thermo Fisher Scientific | |||||||||
| HindIII-HF | New England BioLabs | |||||||||
| Hinfl, recombinant | New England BioLabs | |||||||||
| HpaII | New England BioLabs | |||||||||
| KpnI-HF | New England BioLabs | |||||||||
| MfeI-HF | New England BioLabs | |||||||||
| MlyI, recombinant | New England BioLabs | |||||||||
| NheI-HF | New England BioLabs | |||||||||
| NotI-HF | New England BioLabs | |||||||||
| PacI | New England BioLabs | |||||||||
| PstI-HF | New England BioLabs | |||||||||
| PvuI-HF | New England BioLabs | |||||||||
| SalI-HF | New England BioLabs | |||||||||
| Sau3AI | New England BioLabs | |||||||||
| SpeI-HF | New England BioLabs | |||||||||
| XbaI | New England BioLabs |
Equipment
| Equipment | Name | Manufacturer | ||
|---|---|---|---|---|
| Table Centrifuge | Microfuge® 18 Centrifuge | Beckman CoulterTM | ||
| Table Centrifuge | Microfuge® 22R Centrifuge | Beckman CoulterTM | ||
| Centrifuge | Allegra X-12R | Beckman CoulterTM | ||
| Shaker | VORTEXGENIE 2 | Scientific Industries, SITM | ||
| Heating plate (magnet stirrer) | MR Hei-Standard | Heidolph | ||
| Heatblock | QBD2 | Grant | ||
| Heatblock | QBD4 | Grant | ||
| Heatblock (shaking function) | Thermomixer comfort | Eppendorf | ||
| PCR-Machine | MyCycler thermo cycler | BioRad | ||
| PCR-Machine | T100 Therma Cycler | BioRad | ||
| PCR-Machine | Mastercycler nexus X2 | Eppendorf | ||
| Gel documentation | Transluminator | Vilber Lourmat | ||
| Scale (fine) | PioneerTM PA114C | OHAUS | ||
| Scale | PioneerTM PA4101C | OHAUS | ||
| Incubator | KBW 400 | Binder | ||
| Incubator/ Shaker | ISF1-X | Kuhner shaker | ||
| Incubator / Shaker | HT Multitron Version 2 | INFORS | ||
| Plate Reader | Infinite M200 | Tecan | ||
| Plate Reader | GloMax® Discover | Promega | ||
| UV/VIS Spectrometer | Ultrospec 3300 pro | Amersham Biosciences | ||
| Microvulume spectrometer | NanoPhotometer NP80 | Implen | ||
| PH-Meter | PH-Meter 765 Calimatic | Knick | ||
| Lyophilizer | Gefriertrocknungsanlage ALPHA 1-2 LD Plus Best. Nr. 101521 | Christ | ||
| Sonificator | Sonoplus Gm 2070 (2002) | Bandelin | ||
| Vacuum Manifold | Qiavac 24 Plus | Qiagen | ||
| HPLC | HPLC system with binary pump, VWD detector and autosampler | Knauer | ||
| Nucleic acid purification system | QIAcube | Qiagen |
Kits
| Name | Supplier | ||
|---|---|---|---|
| QIAquick® Gel Extraction Kit | Qiagen | ||
| QIAquick® PCR Purification Kit | Qiagen | ||
| QIAprep® Spin Miniprep Kit | Qiagen | ||
| DNA Clean & Concentrator™-5 | Zymo Research | ||
| ZymoPURE™ Plasmid Miniprep Kit | Zymo Research | ||
| ZymoPURE™ Plasmid Midiprep Kit | Zymo Research | ||
| NEB iGEM Support Kit 2 | New England Biolabs | ||
| NEB Quick Cloning Box-HF | New England Biolabs | ||
| HiScribe T7 Quick High Yield RNA Synthesis Kit | New England Biolabs | ||
| MinION Basic starter pack (1 MinION, 2 Flow cells, 1 Sequencing Kit, 1 Wash kit) | Oxford Nanopore |
Markers
| Name | Supplier | ||
|---|---|---|---|
| Quick-Load 2-Log DNA Ladder (0.1-10.0 kb) | New England BioLabs | ||
| Quick-Load 1 kb DNA Ladder | New England BioLabs | ||
| 100 bp DNA Ladder | New England BioLabs | ||
| Novex® Sharp Pre-stained Protein Standard | Invitrogen | ||
| SERVA Triple Color Protein Standard | SERVA Electrophoresis |
Media
| Media | Composition | |
|---|---|---|
| LB medium (Luria/Miller für d-2,5 kg Best. Nr.: X968.3 | 10 g NaCl, 10 g tryptone/ peptone, 5 g yeast extract in 1 L dH2O | |
| 2xYT medium für die Molekularb-2,5 kg Best. Nr.: X966.3 | 5 g NaCl, 16 g tryptone/ peptone, 10 g yeast extract in 1 L dH2O | |
| SOB medium | 0,5 % yeast extract, 2 % tryptone/peptone, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4 | |
| SOB medium | SOB supplemented with 20 mM glucose | |
| M9 minimal medium |
Other Enzymes
| Name | Supplier | ||||||
|---|---|---|---|---|---|---|---|
| Gibson Assembly® Master Mix | New England Biolabs | ||||||
| T4 DNA Ligase | New England Biolabs | ||||||
| T7 RNA Polymerase | New England Biolabs | ||||||
| T4 Polynucleotide Kinase (PNK) | New England Biolabs | ||||||
| T4 RNA Ligase 1 (ssRNA Ligase) | New England Biolabs | ||||||
| Alkaline Phosphatase (CIP) | New England Biolabs | ||||||
| Shrimp Alkaline Phosphatase (rSAP) | New England Biolabs |
PACE
| Product | Source | Catalog # |
|---|---|---|
| Turbidostat | ||
| Bioreactor 2L MyFerm III | HLL | L14023002 |
| Screw caps with borehole GL45 | Carl Roth | L994.1 |
| Septa for screw caps with borehole GL45 | Carl Roth | LL52.1 |
| Septa for screw caps with borehole GL32 | Carl Roth | LL51.1 |
| Screw caps with borehole GL32 | Carl Roth | H396.1 |
| Tubing,pharmed,2.79mm ID, 100´ | Cole Parmer, Inc | 95809-48 |
| Male luer with lock ring x 1/8" hose barb, PP, 25/pk | Cole Parmer, Inc | 45503-04 |
| Female luer x 1/8" hose barb adapter, PP, 25/pk | Cole Parmer, Inc | 45500-04 |
| Screw caps with borehole GL18 | Carl Roth | EL61.1 |
| Septa for screw caps with borehole GL18 | Carl Roth | LL49.1 |
| GL 18 pressure equaliser set | Carl Roth | KE58.1 |
| GL 18 screw cap | Carl Roth | E696.1 |
| Lab screw fittings 3,2 mm outer diameter | Carl Roth | KE53.1 |
| Bottle multi-dispensers, made of PP | Carl Roth | HX85.1 |
| Rotilabo®-syringe filters, PTFE, sterile, Ø 25 mm, 0.2 µm | Carl Roth | KC76.1 |
| Lagoons | ||
| Fisherbrand™ Reusable Glass Media Bottles with Cap 250 ml | Fisherscientific | 15456113 |
| Septa for screw caps with borehole GL45 | Carl Roth | LL52.1 |
| Screw caps with borehole GL45 | Carl Roth | L994.1 |
| BD 23G 1 in. - 0.6 mm x 25 mm | BD | 305892 |
| Rotilabo®-syringe filters, PTFE, sterile, Ø 25 mm, 0.2 µm | Carl Roth | KC76.1 |
| Tubing,pharmed,0.89mm ID, 100ft | Cole Parmer, Inc | 95809-26 |
| Screw caps with borehole GL14 | Carl Roth | L993.1 |
| Septa for screw caps with borehole GL14 | Carl Roth | LL48.1 |
| GL 14 pressure equaliser set | Carl Roth | HX90.1 |
| GL 14 screw cap | Carl Roth | E695.1 |
| GL 14 laboratory screw joint for hose with 1,6 outer diameter | Carl Roth | HX86.1 |
| GL 14 laboratory screw joint for hose with 3,0 mm outer diameter | Carl Roth | HX87.1 |
| Waste | ||
| Flaschen mit Quetschhahn 20 lt- Weich-Polyethylen (PE-LD), viereckig | Semadeni Plastic Group | 1035 |
| BD 23G 1 in. - 0.6 mm x 25 mm | BD | 305892 |
| Peristaltic pump (lagoon line) | ||
| L/S brushless programmable drive | Cole Parmer, Inc | 07550-50 |
| L/S 8-channel pump head for microbore tubing | Cole Parmer, Inc | 07534-08 |
| Peristaltic pump (media line) | ||
| Masterflex L/S economy variable drive | Cole Parmer, Inc | 07554-80 |
| L/S 8-channel, 3-roller pump head | Cole Parmer, Inc | 07519-05 |
| Masterflex L/S small catridges | Cole Parmer, Inc | 07519-80 |
| Esvelt medium | ||
| Carboys, made of PP 20 L | Carl Roth | C480.1 |
| valve control | ||
| 3-way valve | Conrad | 100P3MP24-05S |
| syringe pump | ||
| Tubing,pharmed,0.89mm ID, 100ft | Cole Parmer, Inc | 95809-26 |
| Microbore two-stop tube sets, silicone; 0.89 mm ID | Cole Parmer, Inc | GZ-06421-26 |
| BD 23G 1 in. - 0.6 mm x 25 mm | BD | 305892 |
| Perfusor® compact | Bbraun | 8714827 |
| media line | ||
| Microbore two-stop tube sets, silicone; 2.06 mm ID | Cole Parmer, Inc | 06421-42 |
| Tubing,pharmed,2.06mm ID, 100ft | Cole Parmer, Inc | 95809-42 |
| Female luer x 1/16" hose barb adapter, PP, 25/pk | Cole Parmer, Inc | 45500-00 |
| Male luer with lock ring x 1/16" hose barb, PP, 25/pk | Cole Parmer, Inc | 45503-00 |
| TuFlux® SIL - TUBE 1/8 ID X 1/4 OD | Sartorius | FSA117425 |
| lagoon line | ||
| Microbore two-stop tube sets, silicone; 1.42 mm ID | Cole Parmer, Inc | 06421-34 |
| Male luer with lock ring x 3/32" hose barb, PP, 25/pk | Cole Parmer, Inc | 45503-02 |
| Female luer x 3/32" hose barb adapter, PP, 25/pk | Cole Parmer, Inc | 45500-02 |
| Tubing,pharmed,1.42mm ID, 100ft | Cole Parmer, Inc | SI-06460-34 |
| O2 line | ||
| Sartopore® Air Midisart® | Sartorius | 17805--------BVN |
| ON-Line measurement | ||
| 5 m Sensor Cable | Hamilton | 243870 |
| Incyte Pre-Amp | Hamilton | 243720 |
| Cell Density ComBox | Hamilton | 243810 |
| Incyte sensor DN12 - 220 mm | Hamilton | 243733 |
Polymerases
| Name | Supplier | ||||||
|---|---|---|---|---|---|---|---|
| OneTaq® Quick-Load® 2X Master Mix with Standard Buffer | New England Biolabs | ||||||
| Phusion Flash High-Fidelity PCR Master Mix | Thermo Fisher Scientific | ||||||
| Q5® High-Fidelity DNA Polymerase 2X Master Mix | New England Biolabs |
