Difference between revisions of "Team:Paris Bettencourt"

Line 38: Line 38:
  
 
<img style="opacity:0.5" src="http://maisonchateauneuf.fr/medusa/images/header-home-animado.gif?crc=3964752341"/>
 
<img style="opacity:0.5" src="http://maisonchateauneuf.fr/medusa/images/header-home-animado.gif?crc=3964752341"/>
−
<div id=abstract><b>Project Description</b></br></div>
+
<div id=abstract><b>Medusa: Bringing control to the third dimension</b></br></div>
  
−
<div id=abstract>From spider silk to mollusc nacre, living organisms have evolved of a wide variety with astonishing physical properties. The synthesis of biomaterials occurs thanks to a sequence of localized physiological events which have remained difficult to harness artificially.</br>
+
<div id=abstract>Accurate spatial-temporal response is fundamental to synthetic biology. Optogenetics has emerged as a powerful tool for genetic control and Medusa brings optogenetics to the next level. By engineering E. coli to respond to multiple light inputs, creating a logical AND gate, we aim to achieve both spatial and temporal control of gene expression. Photosensory transmembrane proteins as well as photoswitchable protein caging were investigated to further expand the existing library of optogenetic tools. For spatial control at the subcellular level, we explored the use of a novel synthetic RNA organelles to manipulate enzymatic activity. Finally, in an effort to promote synthetic biology, we sought the input of the DIY community and chose to illustrate the power of our system by 3D-printing biomaterials.</div>
−
</br>
+
−
+
−
Our iGEM project aims to engineer <i>E. coli</i> to enable a greater spatial and timing control necessary to
+
−
the synthesis of biomaterials. To do so, three research axes will be developed.
+
−
The first axis will focus on the synthesis of biomaterials such as biominerals
+
−
and biopolymers. The second axis will investigate the use of recently described
+
−
RNA scaffolding to optimize the enzymatic catalysis involved in the materials synthesis.
+
−
The third axis will develop the optogenetic circuits enabling the light induced
+
−
control of our bacteria. In its most advanced state, our technology will enable
+
−
us to define the 3D shape and to control the composition (and, hence, the physical
+
−
properties) of our biomaterial.</div>
+
−
</br>
+
−
</br>
+
 
<div id=abstract><b>Contact us</b> </br>bettencourt.igem2017@gmail.com</br>
 
<div id=abstract><b>Contact us</b> </br>bettencourt.igem2017@gmail.com</br>
  

Revision as of 15:25, 18 September 2017

iGEM Paris-Bettencourt 2017


Medusa: Bringing control to the third dimension
Accurate spatial-temporal response is fundamental to synthetic biology. Optogenetics has emerged as a powerful tool for genetic control and Medusa brings optogenetics to the next level. By engineering E. coli to respond to multiple light inputs, creating a logical AND gate, we aim to achieve both spatial and temporal control of gene expression. Photosensory transmembrane proteins as well as photoswitchable protein caging were investigated to further expand the existing library of optogenetic tools. For spatial control at the subcellular level, we explored the use of a novel synthetic RNA organelles to manipulate enzymatic activity. Finally, in an effort to promote synthetic biology, we sought the input of the DIY community and chose to illustrate the power of our system by 3D-printing biomaterials.
Contact us
bettencourt.igem2017@gmail.com