Team:Paris Bettencourt/Part Collection

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  • Membrane Photoreceptor
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@iGEM_Paris

PART COLLECTION

Part Collection

The parts in this collection were designed to form either AND or NOR gates depending on light inputs. Photo-switchable proteins that alternate between homodimer and monomer conformations under specific wavelengths were designed to flank a protein of interest. This creates a protein cage, sterically repressing activity when no light is applied. Inserting either the T7 RNA polymerase or one of three repressors - TetR, HKcI and P22c2 (Parts BBa_K2510100 to BBa_K2510113) - gave us a number of photo-switchable transcription factors. To create logic, we combined such proteins with our library of 12 dually repressible promoters (Parts: BBa_K2510018 to BBa_K2510029), consisting of a single T7 promoter core and two synthetic orthogonal operators. For the first time, we are able to reversibly control gene expression at a high spatio-temporal resolution with easy procedures and minimal cost to the cell.

Part Name	Part Type	Keyword	Designer	Length
BBa_K2510100	Coding	pdDronpa wt	Aya Gomaa	1490
BBa_K2510101	Coding	PDDronpa wt t7 promoter	Aya Gomaa	1540
BBa_K2510102	Coding	pdDronpa mutant	Aya Gomaa	1490
BBa_K2510103	Coding	lacz-pdDronpa	Aya Gomaa	4584
BBa_K2510104	Coding	T7pol-pdDronpa wt under Anderson promoter	Aya Gomaa	4161
BBa_K2510105	Coding	T7pol-pdDronpa wt under t7 promoter	Aya Gomaa	4210
BBa_K2510106	Coding	T7pol-pdDronpa mutant under t7 promoter	Aya Gomaa	4161
BBa_K2510107	Coding	mut Dronpa t7 pol phusion + t7 mrfp part	Aya Gomaa	5063
BBa_K2510108	Coding	pdDronpa wt TetR	Aya Gomaa	2182
BBa_K2510109	Coding	pdDronpa mut TetR	Aya Gomaa	2133
BBa_K2510110	Coding	pdDronpa wt HKCI	Aya Gomaa	2266
BBa_K2510111	Coding	pdDronpa mut HKCI	Aya Gomaa	2217
BBa_K2510112	Coding	pdDronpa wt P22C2	Aya Gomaa	2242
BBa_K2510113	Coding	pdDronpa mut P22c2	Aya Gomaa	2193
BBa_K2510018	Regulatory	T7 - O-HKcI - O-P22c2 promoter	Alma Chapet--Batlle	74
BBa_K2510019	Regulatory	T7 - O-HKcI - O-TetR promoter	Alma Chapet--Batlle	74
BBa_K2510020	Regulatory	T7 - O-P22c2 - O-HKcI promoter	Alma Chapet--Batlle	74
BBa_K2510021	Regulatory	T7 - O-P22c2 - O-TetR promoter	Alma Chapet--Batlle	84
BBa_K2510022	Regulatory	T7- O-TetR - O-HKcI promoter	Alma Chapet--Batlle	74
BBa_K2510024	Regulatory	O-HKcI - T7 - O-HKcI - O-P22c2 promoter	Alma Chapet--Batlle	95
BBa_K2510025	Regulatory	O-HKcI - T7 - O-HKcI - O-TetR promoter	Alma Chapet--Batlle	95
BBa_K2510026	Regulatory	O-P22C2 - T7 - O-P22C2 - O-HKcI promoter	Alma Chapet--Batlle	107
BBa_K2510028	Regulatory	O-TetR - T7 - O-TetR - O-HKcI promoter	Alma Chapet--Batlle	115
BBa_K2510029	Regulatory	O-TetR - T7 - O-TetR - O-P22C2 promoter	Alma Chapet--Batlle	125

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
bettencourt.igem2017@gmail.com