Team:Paris Bettencourt/Results

RESULTS

Optogenetic

Membrane photosensor

  • PHA production under two light AND-gate.
  • Characterisation of slow growth in multi-plasmid system.
  • Chacterisation of AND-gate using transmembrane photoreceptors.

Optics

  • Results confirm the feasibility of achieving a high targeting resolution at significant length within the gel medium.

Biomaterial

Calcium Carbonate

  • Coral acid-rich proteins(CARP1-CARP4) were implemented into the expression vector and transformed into Escherichia coli BL21.
  • Right size proteins were expressed and checked for calcium carbonate precipitation ability.

Polysilicate

  • Characterized a biobrick from iGEM Pasteur

PHA

  • Improved the characterization of part BBa_K1149051 using flow cytometry to better quantify P3HB production in E. coli.

RNA organelle

  • RNA organelle was shown to decrease background expression of GFP, giving evidence to our model.
  • The RNA organelle was long-living, as it was observed to irreversible and stable in culture 20h after induction.

    Logic Gate & Photo-Caging

    • Successful testing in cell-free system with Dronpa.
    • Found evidence that Dronpa "sticks" the repressors to DNA.

    Human Practice

    • Collected feedback from Makerspace and Fablabs to improve our product design.
    • Assessed the safety measures of 3D bio-printing through interviews with specialists.
    • Built an Escape Room game to teach a general audience about synthetic biology and safety issues
    Centre for Research and Interdisciplinarity (CRI)
    Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
    Paris Descartes University
    24, rue du Faubourg Saint Jacques
    75014 Paris, France
    bettencourt.igem2017@gmail.com