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<p>We constructed the Evaluation Vector (EV) to quickly screen for the secretion of a protein of interest as a composite part containing a specifically/specially designed MCS and transformation success indicators based on the pBS1C backbone<sup>1</sup> (Figure 3). Additionally, this vector can be applied for the expression of any other fusion protein of interest regulated by a promoter of your choice. For more details on the application check out our <a target="_blank" href="https://2017.igem.org/Team:TU_Dresden/Measurement">Signal Peptide Toolbox</a> and <a target="_blank" href= "https://2017.igem.org/Team:TU_Dresden/Project/Secretion">Secretion project</a>.</p> | <p>We constructed the Evaluation Vector (EV) to quickly screen for the secretion of a protein of interest as a composite part containing a specifically/specially designed MCS and transformation success indicators based on the pBS1C backbone<sup>1</sup> (Figure 3). Additionally, this vector can be applied for the expression of any other fusion protein of interest regulated by a promoter of your choice. For more details on the application check out our <a target="_blank" href="https://2017.igem.org/Team:TU_Dresden/Measurement">Signal Peptide Toolbox</a> and <a target="_blank" href= "https://2017.igem.org/Team:TU_Dresden/Project/Secretion">Secretion project</a>.</p> | ||
<p>We provide the same construct stored in the pSB1C3 backbone as the <a target="_blank" href="http://parts.igem.org/Part:BBa_K2273107">BioBrick BBa_K2273107.</a></p> | <p>We provide the same construct stored in the pSB1C3 backbone as the <a target="_blank" href="http://parts.igem.org/Part:BBa_K2273107">BioBrick BBa_K2273107.</a></p> | ||
+ | <p>After various adjustments, we established the following protocol for cloning with the EV which we recommend to follow.</p> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td style="width:5%;"><p>1</p></td> | ||
+ | <td style="width:95%;"><p>J Radeck, K Kraft, J Bartels, T Cikovic, F Dürr, J Emenegger, S Kelterborn, C Sauer, G Fritz, S Gebhard and T Mascher "The <i>Bacillus</i> BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with <i>Bacillus subtilis</i>" Journal of Biological Engineering 7:29 (2013). <a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/24295448">PubMed</a></p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width:5%;"><p>1</p></td> | ||
+ | <td style="width:95%;"><p>J Radeck, K Kraft, J Bartels, T Cikovic, F Dürr, J Emenegger, S Kelterborn, C Sauer, G Fritz, S Gebhard and T Mascher "The <i>Bacillus</i> BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with <i>Bacillus subtilis</i>" Journal of Biological Engineering 7:29 (2013). <a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/24295448">PubMed</a></p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width:5%;"><p>1</p></td> | ||
+ | <td style="width:95%;"><p>J Radeck, K Kraft, J Bartels, T Cikovic, F Dürr, J Emenegger, S Kelterborn, C Sauer, G Fritz, S Gebhard and T Mascher "The <i>Bacillus</i> BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with <i>Bacillus subtilis</i>" Journal of Biological Engineering 7:29 (2013). <a target="_blank" href ="https://www.ncbi.nlm.nih.gov/pubmed/24295448">PubMed</a></p></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p>Additionally, a second SOP was tailored to explain the random integration of secretion signal peptides as the EV was evaluated in the course of our <a target="_blank" href="https://2017.igem.org/Team:TU_Dresden/Measurement"> Signal Peptide Toolbox</a>.</p> | ||
</div> | </div> | ||
</main> | </main> |
Revision as of 15:33, 24 October 2017