Team:TU Dresden/Experiments
Project
Peptidosomes
β-Lactam Biosensor
Signal Peptide Toolbox
Evaluation Vector
Secretion
Communication
Results
Demonstration
Parts
Human Practices
Outreach
Integrated
Collaborations
iGEM goes green
Green Teams
Resources
Our Achievements
Our Experience
About Us
Team
Attributions
Financing & Sponsors
Lab Work
Experiments & Protocols
Safety
Judging
Don´t try this at home
Peptidosome Methods
Peptidosome Creation
Encapsulation of
B. subtilis
Plate Reader Wellscan Peptidosomes
Cloning and Primer Lists
Cloning
Cloning Procedure
Transformation CaCl Method
PCR with One Taq Polymerase
PCR with Q5 Polymerase
Long Flanking Homology PCR
SOP Protocols for Cloning with the Evaluation Vector and the Signal Peptide Toolbox
Primer
Primer List General
Primer List Signal Peptides
Working with
Bacillus subtilis
DNA Isolation (for PCR)
DNA Isolation (for Transformation)
Transformation of
B. subtilis
Media and Solutions
Media, Solutions & Antibiotics
Jensen's Medium
MCSE Medium
Assays
Disc Diffussion Assay
Plate Reader Assay
Fluorescence Assay
Protein Work
His-Tag Purification with Agarose Beads
Laemmli SDS-PAGE
