Line 63: | Line 63: | ||
<tr> | <tr> | ||
<td style="width:5%;"><p>1</p></td> | <td style="width:5%;"><p>1</p></td> | ||
− | <td style="width:95%;"><p> | + | <td style="width:95%;"><p>Exchange of the promoter: Digest both, the provided EV and the new promoter using the restriction enzymes EcoRI and BsaI.<br>We recommend to purify all digested products via gel extraction.</p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td style="width:5%;"><p> | + | <td style="width:5%;"><p>2</p></td> |
− | <td style="width:95%;"><p> | + | <td style="width:95%;"><p>Insertion of the C-terminally fused protein: Digest both, the EV from step 1 and the new gene of interest using the restriction enzymes NgoMIV and PstI.<br>We recommend to purify all digested products via gel extraction.</p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td style="width:5%;"><p> | + | <td style="width:5%;"><p>3</p></td> |
− | <td style="width:95%;"><p> | + | <td style="width:95%;"><p>Insertion of the N-terminally fused protein: Digest the EV from step 2 using the restriction enzymes BsaI and NgoMIV BUT digest the gene of interest using the restriction enzymes XbaI and AgeI.<br>We recommend to purify all digested products via gel extraction.</p></td> |
</tr> | </tr> | ||
</table> | </table> |
Revision as of 15:35, 24 October 2017