Difference between revisions of "Team:AshesiGhana"

 
(48 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
{{Template:AshesiGhana/NavBar}}
 
{{Template:AshesiGhana/NavBar}}
−
<html>
+
<html class="no-js">
−
 
+
    <head>
−
<head>
+
−
    <title>Header</title>
+
 
     <meta charset="utf-8">
 
     <meta charset="utf-8">
−
     <meta name="viewport" content="width=device-width, initial-scale=1">
+
     <meta http-equiv="X-UA-Compatible" content="IE=edge">
−
    <link rel="stylesheet" href="http://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
+
−
    <link rel="stylesheet" type="text/css" href="https://ashbuspay.000webhostapp.com/assets/css/Header-Nightsky.css">
+
−
    <link href="https://fonts.googleapis.com/css?family=Montserrat" rel="stylesheet">
+
  
−
     <style>
+
     <meta property="og:title" content=""/>
−
     #home_logo, #sideMenu { display:none; }
+
     <meta property="og:image" content=""/>
−
     #sideMenu, #top_title, .patrollink  {display:none;}
+
     <meta property="og:url" content=""/>
−
     #content { width:100%; padding:0px;  margin-top:-13px; margin-left:0px;}
+
     <meta property="og:site_name" content=""/>
−
     #headerContainer { width:100%}
+
    <meta property="og:description" content=""/>
−
     body {background-color:white; }
+
    <meta name="twitter:title" content="" />
−
     #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
+
     <meta name="twitter:image" content="" />
 +
     <meta name="twitter:url" content="" />
 +
     <meta name="twitter:card" content="" />
  
 +
       
 +
        <style>
 +
        .bck {
 +
    background-image: url(https://static.igem.org/mediawiki/2017/2/28/T--AshesiGhana--backgroundPattern.jpg);
 +
    background-repeat: repeat;
 +
    padding-bottom: 10px;
 +
}
 +
.shell-custom {
 +
    margin-right: 0;
 +
    margin-left: 0;
 +
    min-width: 100%;
 +
}
 +
    .row *{
 +
    font-family: 'Nunito' !important;
 +
}
 
</style>
 
</style>
−
</head>
+
       
  
 +
 +
    <!-- FOR IE9 below -->
 +
    <!--[if lt IE 9]>
 +
    <script src="js/respond.min.js"></script>
 +
    <![endif]-->
 +
 +
    </head>
 +
    <body>
 +
 +
 +
 +
</head>
 
<body>
 
<body>
−
     <div class="header-nightsky">
+
 
−
         <nav class="navbar navbar-default">
+
 
−
             <div class="headerContainer">
+
     <header>
−
                 <a class="navbar-brand" href="#">LOGO</a> <img src="[[File:LogoGiGEM.png]]" alt="GiGEM Logo" style="width:304px;height:228px;">
+
  <!--Banner starts here-->
−
                <div class="navbar-header">
+
    <section id="banner">
−
                    <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#myNavbar">
+
 
−
                         <span class="icon-bar"></span>
+
         <div class="row">
−
                        <span class="icon-bar"></span>
+
             <div class="col-md-12">
−
                        <span class="icon-bar"></span>
+
                 <div id="bg-slider" class="carousel slide" data-ride="carousel">
−
                     </button>
+
 
 +
                    <!-- Wrapper for slides -->
 +
                    <div class="carousel-inner" role="listbox" style="height: 700px;">
 +
                      <div class="item active one">
 +
                            <div class="carousel-caption">
 +
                                <div class = "shell-custom bck">
 +
                                    <h2>Meet the team solving a national crisis</h2>
 +
                                    <p>
 +
                                        This is a team of bright scientists and engineers who have gathered the courage to engineer a biological solution for one of their nation's biggest problems. 
 +
                                    </p>
 +
                                </div>
 +
                            </div>
 +
 
 +
                        </div>
 +
                        <div class="item two">
 +
                           
 +
 
 +
 
 +
                               
 +
            <div class="container">
 +
                <div class="fh5co-intro-wrap">
 +
                    <div class="fh5co-intro">
 +
                         <div class="row">
 +
                            <div class="text-center">
 +
                                <!--<div class="shell-custom bck">
 +
                                    <h1 class="fh5co-intro-lead animate-1 to-animate"> Team<br>AshesiGhana</h1>
 +
                                    <h2 class="fh5co-intro-sub animate-2 to-animate">Introducing the Greatest Gold Miners in the World....Microbes</h2>
 +
                                </div>-->
 +
                            </div>
 +
                        </div>
 +
                     </div>
 
                 </div>
 
                 </div>
−
                <div class="collapse navbar-collapse" id="myNavbar">
+
            </div>
−
                    <ul class="nav navbar-nav navbar-right">
+
<div class="carousel-caption">
−
                        <li><a href="#">Home</a></li>
+
    <div class="shell-custom bck">
−
                        <li class="dropdown">
+
                                <h2>Obuasi Gold Fields </h2>
−
                            <a class="dropdown-toggle" data-toggle="dropdown" href="#">Team <span class="caret"></span></a>
+
                                <p>Mining fields like these have made Ghana  one of the world's leading producers of Gold. However the scent of Gold attracts people who do not always have much care for the environment. Learn More About <a href = "https://en.wikipedia.org/wiki/Galamsey">Galamsey</a></p>
−
                            <ul class="dropdown-menu">
+
                             </div>
−
                                            <li><a href="">Meet The Miners</a></li>
+
                             </div>
−
                                            <li><a href="">Nouguchi Heros</a></li>
+
                         </div>
−
                                        </ul>
+
                         <div class="item three">
−
                        </li>
+
                             <div class="carousel-caption">
−
                        <li class="dropdown">
+
                                <div class="shell-custom bck">
−
                            <a class="dropdown-toggle" data-toggle="dropdown" href="#">Project <span class="caret"></span></a>
+
                                    <h2>Pioneer Team from Ghana</h2>
−
                            <ul class="dropdown-menu">
+
                                    <p>From a well meshed background of Engineering and Computer Science, unfazed by the challenges of Synthetic Biology and fuelled by a drive to Solve Problems that impact our scoiety. </p>
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Description">Description</a></li>
+
                                </div>
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Description">Proof of Concept</a></li>
+
                             </div>  
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Description">Results</a></li>
+
 
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Description">Safety</a></li>
+
                        </div>
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Description">Interlab</a></li>
+
                    </div>
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Description">Demonstrate</a></li>
+
 
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Description">Improvements</a></li>
+
                      
−
                                        </ul>
+
−
                        </li>
+
−
                        <li class="dropdown">
+
−
                             <a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts <span class="caret"></span></a>
+
−
                          <ul class="dropdown-menu">
+
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Description">Parts</a></li>
+
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Description">Basic &amp; Composite Parts</a></li> <!-- https://2017.igem.org/Team:ColumbiaNYC/Composite_Part-->
+
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Description">Parts Collection</a></li>
+
−
                                        </ul>
+
−
                        </li>
+
−
                        <li class="dropdown">
+
−
                             <a class="dropdown-toggle" data-toggle="dropdown" href="#">Modelling <span class="caret"></span></a>
+
−
                            <ul class="dropdown-menu">
+
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Description">Mathematical Models</a></li>
+
−
                                        </ul>
+
−
                         </li>
+
−
                         <li class="dropdown">
+
−
                             <a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook <span class="caret"></span></a>
+
−
                            <ul class="dropdown-menu">
+
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Brainstorm">Brainstorm</a></li>
+
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Notebook">Lab Notebook</a></li>
+
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Protocols">Protocols</a></li>
+
−
                                        </ul>
+
−
                        </li>
+
−
                        <li class="dropdown">
+
−
                             <a class="dropdown-toggle" data-toggle="dropdown" href="https://2017.igem.org/Team:AshesiGhana/Practices">Human Practices<span class="caret"></span></a>
+
−
                            <ul class="dropdown-menu">
+
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Engagment">Engagment</a></li>
+
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Entrepreneurship">Entrepreneurship</a></li>
+
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Collaborations">Collaborations</a></li>
+
−
                                            <li><a href="https://2017.igem.org/Team:AshesiGhana/Medal">Medal Citerion</a></li>
+
−
                                        </ul>
+
−
                        </li>
+
−
                     </ul>
+
 
                 </div>
 
                 </div>
 +
 
             </div>
 
             </div>
−
        </nav>
 
−
        <div class="hero">
 
−
            <h1>Team AshesiGhana</h1>
 
−
            <h2>Introducing the Greatest Gold Miners in the World....Microbes</h2>
 
−
            <div class="btn btn-primary">Learn more about us</div>
 
−
            <div class="btn btn-primary">Contact us</div>
 
 
         </div>
 
         </div>
−
    </div>
 
−
   
 
−
    <script src="http://ajax.googleapis.com/ajax/libs/jquery/2.1.1/jquery.min.js"></script>
 
−
    <script src="http://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
 
−
</body>
 
  
 +
    </section>
 +
</header>
 +
    <!--Banner ends here-->
 +
 +
    <!--  <header role="banner" id="fh5co-header" style="background-image: url(https://static.igem.org/mediawiki/2017/2/23/GhanaSite.jpg);  background-size: 100% 100%;
 +
    background-repeat: no-repeat;" data-stellar-background-ratio="0.5">
 +
 +
            <div class="fh5co-overlay" ></div>
 +
            <div class="container">
 +
                <div class="fh5co-intro-wrap">
 +
                    <div class="fh5co-intro">
 +
                        <div class="row">
 +
                            <div class="col-md-8 col-md-offset-2 text-center">
 +
                                <h1 class="fh5co-intro-lead animate-1 to-animate">Team AshesiGhana</h1>
 +
                                <h2 class="fh5co-intro-sub animate-2 to-animate">Introducing the Greatest Gold Miners in the World....Microbes</h2>
 +
                               
 +
                            </div>
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
        </header> -->
 +
       
 +
        <main role="main" id="fh5co-main">
 +
            <div class="container" id="bio-animate">
 +
                <div class="row row-padded">
 +
                    <div class="col-md-3">
 +
                        <h2 class="fh5co-section-heading to-animate">Project Description<span class="fh5co-border"></span></h2>
 +
                    </div>
 +
                    <div class="col-md-8 col-md-push-1">
 +
                        <div class="row">
 +
                            <div class="col-md-6 to-animate">
 +
                                <p>For the 2017 IGEM, Team AshesiGhana is working on a bio-mining project. The objective of our project is to develop an organism capable of liberating gold from the refractory ore. We aim to provide not only an alternative, non-toxic approach for small scale mining, but also deliver an easy and fast bio-detection and quantification method of ore in the mining industry of our country. The availability of such a biosensor will allow routine monitoring of the ore before a mining endeavor is undertaken thus preventing the destruction of the environment. The project will engineer the typical environmental organism Acidithiobacillus ferroxidans with a FRET probe.</p>
 +
 +
                                <p>This new organism will primarily be capable of sensing and quantifying the amount of gold in the ore. This will be accomplished by using a two-part probe, a donor part which is made up of gold binding protein (golB) attached to a green fluorescent protein (nowGFP). The second part of the acceptor is also made up of a binding protein and a red fluorescent protein (mRuby2). In the presence of a high amount of free gold, the two parts would be in close proximity and energy transfer can take place and the red protein would be excited giving off a fluorescent signal. Using calibration experiments, we can relate the amount of fluorescence to the amount of gold present,liberated by the organism from the ore.</p>
 +
                            </div>
 +
                            <div class="col-md-6 to-animate">
 +
                                <p>As Acidithiobacillus ferroxidans is a difficult organism to grow in large quantities, we will engineer a strain of E coli to produce two of the main oxidizing enzymes for iron and sulphite which will liberate the gold from the ore. The organism will also be engineered with protective enzymes against the low pH, which is one of the bi-products of the gold liberation reaction, and metal toxicity. The same FRET biosensor part can be added to the E coli strain for the quantification of gold. This new organism can easily be grown in large batches and so can be used to extract gold from refractory ore without the need of any toxic treatment thus providing a safe alternative for small scale mining. </p>
 +
                            </div>
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
                <!-- END .row -->
 +
            </div>
 +
 +
 +
           
 +
 +
        </main>
 +
 +
        <!-- jQuery -->
 +
        <script src="https://ashbuspay.000webhostapp.com/js/jquery.min.js"></script>
 +
        <!-- jQuery Easing -->
 +
        <script src="https://ashbuspay.000webhostapp.com/js/jquery.easing.1.3.js"></script>
 +
        <!-- Bootstrap -->
 +
        <script src="https://ashbuspay.000webhostapp.com/js/bootstrap.min.js"></script>
 +
        <!-- Waypoints -->
 +
        <script src="https://ashbuspay.000webhostapp.com/js/jquery.waypoints.min.js"></script>
 +
        <!-- Stellar -->
 +
        <script src="https://ashbuspay.000webhostapp.com/js/jquery.stellar.min.js"></script>
 +
        <!-- countTo -->
 +
        <script src="https://ashbuspay.000webhostapp.com/js/jquery.countTo.js"></script>
 +
        <!-- Magnific Popup -->
 +
        <script src="https://ashbuspay.000webhostapp.com/js/jquery.magnific-popup.min.js"></script>
 +
        <!-- Main JS -->
 +
        <script src="https://ashbuspay.000webhostapp.com/js/main.js"></script>
 +
 +
   
 +
    </body>
 
</html>
 
</html>
 +
{{Template:AshesiGhana/Footer}}

Latest revision as of 19:50, 27 October 2017

Ashesi University
College

Project Description

For the 2017 IGEM, Team AshesiGhana is working on a bio-mining project. The objective of our project is to develop an organism capable of liberating gold from the refractory ore. We aim to provide not only an alternative, non-toxic approach for small scale mining, but also deliver an easy and fast bio-detection and quantification method of ore in the mining industry of our country. The availability of such a biosensor will allow routine monitoring of the ore before a mining endeavor is undertaken thus preventing the destruction of the environment. The project will engineer the typical environmental organism Acidithiobacillus ferroxidans with a FRET probe.

This new organism will primarily be capable of sensing and quantifying the amount of gold in the ore. This will be accomplished by using a two-part probe, a donor part which is made up of gold binding protein (golB) attached to a green fluorescent protein (nowGFP). The second part of the acceptor is also made up of a binding protein and a red fluorescent protein (mRuby2). In the presence of a high amount of free gold, the two parts would be in close proximity and energy transfer can take place and the red protein would be excited giving off a fluorescent signal. Using calibration experiments, we can relate the amount of fluorescence to the amount of gold present,liberated by the organism from the ore.

As Acidithiobacillus ferroxidans is a difficult organism to grow in large quantities, we will engineer a strain of E coli to produce two of the main oxidizing enzymes for iron and sulphite which will liberate the gold from the ore. The organism will also be engineered with protective enzymes against the low pH, which is one of the bi-products of the gold liberation reaction, and metal toxicity. The same FRET biosensor part can be added to the E coli strain for the quantification of gold. This new organism can easily be grown in large batches and so can be used to extract gold from refractory ore without the need of any toxic treatment thus providing a safe alternative for small scale mining.