Difference between revisions of "Team:TU Dresden/Project/Peptidosomes"

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<h1 class="box-heading">Background</h1>
 
<h1 class="box-heading">Background</h1>
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<p>Background
 
  
 
Bacteria are omnipresent in biotechnology and applied projects. They can be used as hosts to produce nearly any biological compound of interest such as: drugs, vaccines, enzymes, antibiotics or even fuels and solvents. Their fast life cycle and comparable low requirements of living conditions highlight their industrial relevance.  
 
Bacteria are omnipresent in biotechnology and applied projects. They can be used as hosts to produce nearly any biological compound of interest such as: drugs, vaccines, enzymes, antibiotics or even fuels and solvents. Their fast life cycle and comparable low requirements of living conditions highlight their industrial relevance.  
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Yet, there are more aspect which need to be considered when producing a compound of interest. First, where is the product of interest found: inside of the producing strain or will it be secreted to the surrounding media? Second, what is necessary to separate the valuable end-product from the bacteria? And maybe most important, how to assure a safe use of genetically engineered production strains?  
 
Yet, there are more aspect which need to be considered when producing a compound of interest. First, where is the product of interest found: inside of the producing strain or will it be secreted to the surrounding media? Second, what is necessary to separate the valuable end-product from the bacteria? And maybe most important, how to assure a safe use of genetically engineered production strains?  
  
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To address these major biological and technical questions, the TU Dresden iGEM team presents EncaBcillus. Using Bacillus subtilis as model organism we introduce a new fundamental approach for cultivation of bacteria: the Peptidsomes. These Peptidosmes are buildup of self-assembled dipeptide phenylalanines (FF) and are able to form spherical cages. The cages hold back the bacteria from the surrounding but are freely diffusible for smaller molecules. This new approach of bacterial immobilization holds several advantages: first, only the crust layer of the Pepdisome is solid, while the inside of a Peptidosome remains in its liquid state allowing the bacteria to freely grow and fulfill their given tasks. Second, due to the physical separation of bacteria and surrounding secreted compounds of interest can be easily harvested without interfering with the bacteria.  
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To address these major biological and technical questions, the TU Dresden iGEM team presents EncaBcillus. Using <i>Bacillus subtilis</i> as model organism we introduce a new fundamental approach for cultivation of bacteria: the Peptidsomes. These Peptidosmes are build-up of self-assembled dipeptide phenylalanines (FF) and are able to form spherical cages. These cages hold back the bacteria from the surrounding but are freely diffusible for smaller molecules. This new approach of bacterial immobilization holds several advantages: first, only the crust layer of the Pepdisome is solid, while the inside of a Peptidosome remains in its liquid state allowing the bacteria to freely grow and fulfill their given tasks. Second, due to the physical separation of bacteria and surrounding secreted compounds of interest can be easily harvested without interfering with the bacteria.  
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We demonstrate the usefulness of peptidosomes with four different fully standalone and working concepts. Links zu den Anwendungen!
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Revision as of 16:49, 28 October 2017

It´s a trap!

Short Description

Peptidosomes are great.

Background

Bacteria are omnipresent in biotechnology and applied projects. They can be used as hosts to produce nearly any biological compound of interest such as: drugs, vaccines, enzymes, antibiotics or even fuels and solvents. Their fast life cycle and comparable low requirements of living conditions highlight their industrial relevance. Over the last decades, the main focus to increase yields laid on extensive metabolic engineering and optimizing growth conditions. Yet, there are more aspect which need to be considered when producing a compound of interest. First, where is the product of interest found: inside of the producing strain or will it be secreted to the surrounding media? Second, what is necessary to separate the valuable end-product from the bacteria? And maybe most important, how to assure a safe use of genetically engineered production strains? To address these major biological and technical questions, the TU Dresden iGEM team presents EncaBcillus. Using Bacillus subtilis as model organism we introduce a new fundamental approach for cultivation of bacteria: the Peptidsomes. These Peptidosmes are build-up of self-assembled dipeptide phenylalanines (FF) and are able to form spherical cages. These cages hold back the bacteria from the surrounding but are freely diffusible for smaller molecules. This new approach of bacterial immobilization holds several advantages: first, only the crust layer of the Pepdisome is solid, while the inside of a Peptidosome remains in its liquid state allowing the bacteria to freely grow and fulfill their given tasks. Second, due to the physical separation of bacteria and surrounding secreted compounds of interest can be easily harvested without interfering with the bacteria.