General Support

• Dr. Hans-Joachim Wieden for administrative, project and wetlab support as a Primary Investigator
• Dr. Trushar Patel for administrative, project and wetlab support as a Secondary Investigator
• Dr. Brian Dempsey for his administrative support and work through the High School iGEM team as well as his efforts in teaching the Synthetic Biology connected to the collegiate iGEM team
• Fan Mo for her wetlab support and for the ordering of consumables therin
• Emily Wilton for her advice and administrative work in our collaboration with Synbridge
• Melissa Wiebe for her collaboration and support with the Shine on Festival.



We would also like to thank the Alberta RNA Research and Training Institute (ARRTI) for their guidence and support of team members as well as their support of our project presentation at the ARRTI Annual General Meeting.

Facilities

• Laboratory Space: Chemistry and Biochemistry Department, Wieden Lab, Patel lab, Synbridge
• Meeting Space: AGILITY Idea Shop

Interviews

• Ian Andrews; Legal Intern at TecEdmonton
• Justin Pahara; Co-founder of Amino Labs
• Julie Legault; Co-founder of Amino Labs
• Sheanne Cox; Middle School Teacher
• Derek Masterman; High School Teacher
• Brent Runnett; Elementary School Principle
• Patrick Shackleford; Teacher at Winston Churchill High School Science Department
• Emily Wilton; Synbridge Coordinator
• Hans Wilms; Biotechnology Consultant, The ODIN
• Kathrina Yambao; Senior Policy Analyst, Public Health Agency of Canada
• Melanie Sabourin; Biosafety Inspector, Public Agency of Canada
• Heather Underwood; Co-founder of Denver BioLabs, DNA Synthesis Company

Collaborations

The Lethbridge High School iGEM Team; High School team website

Florida State University; FSU team website

Gifts-in-kind

Sponsors

Team Attributions

Biosecurity

• Chris Isaac: DNA synthesis company contact, CODONxCHANGE software suite
• Sydnee Calhoun: Regulations research
• Morag Runnett: Preliminary regulation research

Construct Design

• Harland Brandon
• Graeme Glaister
• Taylor Sheahan
• Dustin Smith
• Initial Translation Components Construct Compilation (sequence retrieval and codon optimization): Simmone D'Souza, Regan Evanson, cynthia Fonderson, Josh Friesen,Jonathon Hampshire, Lane Harper, Chelsi Harvey, Rebecca Jeong, Chris Lowen, Derrik Motz,Morag Runnett, Jessica Semmelrock, Sandhya Sunuwar, Kristi Turton

Education

• Keith Aiken: Education kit protocol alignment with Alberta high school curriculum
• Sydnee Calhoun: Professional Development Day contact with SynBridge and high schools, A Student’s/Teacher’s Guide to: Next vivo Transcription and Translation Education Kit, Next vivo, A guide to: Biosafety
• Michelle Cradduck: A Student’s/Teacher’s Guide to: Next vivo Transcription and Translation Education Kit
• Chelsi Harvey: A Student’s/Teacher’s Guide to: Next vivo Transcription and Translation Education Kit, Next vivo, A guide to: Biosafety, Education kit schematic, Module Design
• Chris Isaac: A Student’s/Teacher’s Guide to: Next vivo Transcription and Translation Education Kit
• Morag Runnett: Preliminary curriculum research
• Dustin Smith:A Student’s/Teacher’s Guide to: Next vivo Transcription and Translation Education Kit

Funding

• Harland Brandon: Alberta Innovates proposal
• Sydnee Calhoun: QIP proposal and presentation content, GoFundMe management
• Simmone D'Souza: QIP proposal and presentation
• Reagan Evanson: General funding
• Cynthia Fonderson: QIP proposal and presentation
• Graeme Glaister: Alberta Innovates Proposal, QIP proposal and presentation content
• Chelsi Harvey: QIP presentation content, General funding
• Taylor Sheahan: Alberta Innovates proposal
• Dustin Smith: Alberta Innovates proposal
• Sandhya Sunuwar: QIP proposal and presentation

Interviews

• Keith Aiken: Amino Bio Labs
• Harland Brandon: DNA synthesis company
• Sydnee Calhoun: Amino Bio Labs, The ODIN, Denver Bio Labs, Ian Andrews, Public Health Agency of Canada, Winston Churchill High School Department, Dr. Ute Wieden-Kothe, Emily Wilton
• Graeme Glaister: System standardization company
• Chris Isaac: Amino Bio Labs, Ian Andrews, Public Health Agency of Canada, five DNA synthesis companies
• Morag Runnett: Amino Bio Labs, Brent Runnett, Emily Wilton
• Taylor Sheahan: Ian Andrews, system standardization company, DNA synthesis company
• Kristi Turton: Denver Bio Labs, Derek Masterman

Miscellaneous (Media, Design, Administrative)

• Keith Aiken: Team social media, photographer, videographer, meeting minutes
• Harland Brandon: Wet-lab training sessions, protein overexpression, and protein purification tutorials
• Michelle Cradduck: Cloning tutorials
• Josh Friesen: protein overexpression, and protein purification tutorials
• Graeme Glaister: Safety forms
• Chelsi Harvey: Next vivo and Next vivo 2.0 logos, project name (Next vivo), and figure design
• Chris Isaac: Banner design, software figures
• Taylor Sheahan: Wet-lab training sessions, room bookings for all meetings, registration for events, protein overexpression, and protein purification tutorials
• Dustin Smith: Cloning, protein overexpression, and protein purification tutorials
• Kristi Turton: Ordered team t-shirts

Poster

• Harland Brandon: Content, editing
• Sydnee Calhoun: Content, editing
• Chelsi Harvey: Content, editing, figures
• Chris Isaac: Content, editing, figures
• Taylor Sheahan: Content, editing
• Presenters: Sydnee Calhoun, Josh Friesen, Chelsi Harvey, Chris Isaac, Sandhya Sunuwar, Kristi turton

Presentation

• Harland Brandon: Content
• Sydnee Calhoun: Content
• Chelsi Harvey: Content, design, figures
• Chris Isaac: Content, design, figures
• Taylor Sheahan: Content
• Presenters: Sydnee Calhoun, Chelsi Havey, Chris Isaac

Public Engagement

• Keith Aiken: 10th anniversary outreach
• Harland Brandon: ARRTI Annual General Meeting poster content, Chinook Symposium poster content, aGEM presentation content
• Sydnee Calhoun: Shine On Festival, ARRTI Annual General Meeting poster content, Chinook Symposium poster content and presenter, aGEM presenter, aGEM presentation content
• Simmone D'Souza: ARRTI Annual General Meeting poster presenter
• Cynthia Fonderson: ARRTI Annual General Meetin poster presenter
• Josh Friesen: Chinook Symposium presenter
• Graeme Glaister: Brick-by-brick
• Chelsi Harvey: aGEM presenter, aGEM presentation content, ARRTI Annual General Meeting poster content, Chinook Symposium poster content and presenter, figures and formatting for aGEM presentation and AAGM Chinook Symposium posters
• Chris Isaac: aGEM presenter, aGEM presentation content, ARRTI Annual General Meeting Poster content, Chinook Symposium poster content and presenter, Shine On Festival presenter, figures for AAGM and Chinook Symposium posters
• Chris Lowen: ARRTI Annual General meeting poster presenter
• Taylor Sheahan: Brick-by-brick, ARRTI Annual General Meeting poster content, Chinook Symposium poster content, aGEM presentation content
• Sandhya Sunuwar: Shine on Festival tri-fold
• Kristi Turton: Shine on Festival tri-fold/presenter, Chinook Symposium presenter

Wet-Lab

• Harland Brandon: tRNA, molecular biology, sequencing results, protein overexpressions and purifications
• Josh Friesen: protein overexpressions and purifications, molecular biology
• Graeme Glaister: tRNA, molecular biology
• Lane Harper: molecular biology
• Chris Lowen: initial tRNA expression work and rRNA MS2 tag insertion work
• Will Reid: in vitro transcription of Spinach, molecular biology
• Taylor Sheahan: molecular biology, sequencing results, PURExpress
• Dustin Smith: molecular biology, protein overexpressions and purifications
• Sandhya Sunuwar: cloning, sequencing results, molecular biology
• Kristi Turton: cloning, sequencing results, molecular biology
• Initial Cloning:Michelle Cradduck, Simmone D’Souza, Cynthia Fonderson, Matt Harding, Chelsi Harvey, Derrik Motz, Morag Runnett
• Interlab Study: Michelle Cradduck, Simmone D’Souza, Regan Evanson, Cynthia Fonderson, Jonathan Hampshire, Kristi Turton, Taylor Sheahan, Dustin Smith, Sandhya Sunuwar

Wiki

• Keith Aiken: Wiki framework (Nav bar,headers, footers, majority of coding)
• Harland Brandon: Project (Description, Design, Demonstrate, Results), Parts (Overview, Composite, Part Collection, Improve, Basic)
• Sydnee Calhoun: Project (Collaborations), Human Practices (Overview, Silver, Gold, Engagement), Lab (Safety)
• Michelle Cradduck: Lab (InterLab)
• Graeme Glaister: Parts (Composite, Part Collection, Improve, Basic), Lab (Notebook)
• Chelsi Harvey: Main Page, Project (Description, Design), Parts (Overview, Improve), Human Practices (Overview, Gold), Team (Attributions)
• Chris Isaac: Human Practices (Software)
• Taylor Sheahan: Main Page, Project (Description, Design,Demonstrate, Results), Parts (Overview, Composite, Part Collection, Improve, Basic), Human Practices (Engagement), Lab (InterLab, Safety)
• Dustin Smith: Lab (InterLab)
• Sandhya Sunuwar: Project (Results: Statistics)
• Kristi Turton: Lab (Experiments, Notebook)