document.getElementById("text").innerHTML="<img src='https://static.igem.org/mediawiki/2017/3/35/T--AQA_Unesp--administration.png' id='man_title1'> We want our bacteria to be easily taken: our idea is having a final product that can be ingested by a fermented milk or a lyophilized that can be mixed with other beverages, so it can easily taken by children, adults and elder people without causing any discomforts. Once ingested, the bacteria will be set up in the gut, where it will sense and actuate.";

document.getElementById("text").innerHTML="<img src='https://static.igem.org/mediawiki/2017/d/d7/T--AQA_Unesp--sensing.png' id='man_title2'> We don’t want to produce insulin the whole time  without any control! So, the bacteria needs to sense and control its production. We will build a control system based on the natural L. lactis system for catabolite repression along with regulation by a small RNA (sRNA).<br>The CcpA protein is the protein responsible for control the gene expression in gram-positive bacteria, this protein is activated when glucose is present and then it binds to the DNA at the catabolite repression site (CRE) and represses the expression of downstream gene. We will put a coding sequence for a sRNA under the control of a promoter containing a CRE site, that targets the RBS site and the start codon of the mRNA coding the insulin gene, blocking its translation.<br>This way, when there is no glucose, the expression of our sRNA will be on and will block the production of insulin. Where there is glucose, the expression of our sRNA will be off and we will have the production of insulin. The produced insulin then is ready to be secreted and absorbed.";

document.getElementById("text").innerHTML="<img src='https://static.igem.org/mediawiki/2017/d/d1/T--AQA_Unesp--actuating.png' id='man_title3'> We need our insulin to be secreted by our bacteria so the insulin gene will have an secretion signal sequence called usp45 which allows L. lactis to secrete proteins. The insulin that we will be using is a single-chain analog called SCI-57 that can be produced by bacteria and maintain its biological activity. Once the insulin is out, it needs to be absorbed by the intestinal epithelium, so our insulin will be associated with cell-penetrating peptides (CPPs) that allow the uptake of the insulin, which will reach the blood and then perform its biological function.";