Difference between revisions of "Team:AQA Unesp"

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document.getElementById("text").innerHTML="<img src='https://static.igem.org/mediawiki/2017/d/d7/T--AQA_Unesp--sensing.png' id='man_title2'>Aliquam mattis venenatis est, vel porttitor quam feugiat at. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Curabitur quis ex consectetur tortor dictum hendrerit at quis orci.";
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document.getElementById("text").innerHTML="<img src='https://static.igem.org/mediawiki/2017/d/d7/T--AQA_Unesp--sensing.png' id='man_title2'>We don’t want to produce insulin the whole time  without any control! So, the bacteria needs to sense and control its production. We will build a control system based on the natural L. lactis system for catabolite repression along with regulation by a small RNA (sRNA). <br> The CcpA protein is the protein responsible for control the gene expression in gram-positive bacteria, this protein is activated when glucose is present and then it binds to the DNA at the catabolite repression site (CRE) and represses the expression of downstream gene. We will put a coding sequence for a sRNA under the control of a promoter containing a CRE site, that targets the RBS site and the start codon of the mRNA coding the insulin gene, blocking its translation. <br>  This way, when there is no glucose, the expression of our sRNA will be on and will block the production of insulin. Where there is glucose, the expression of our sRNA will be off and we will have the production of insulin. The produced insulin then is ready to be secreted and absorbed.";
 
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Revision as of 02:48, 30 June 2017



iGEM AQA_Unesp
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Team: AQA_Unesp

Treating diabetes with probiotics


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Team: AQA_Unesp