Difference between revisions of "Team:William and Mary/Parts"

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   <div style='padding-top: 40px;padding-bottom: 40px; font-size: 22px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.</div>
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#sideMenu, #top_title {display:none;}
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div.exp {font:normal 0.9em/1.5em; color:#A9A9A9;}
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<!--UNS pdt#3 DT-->
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<a class="exp" href="#url" tabindex="1">UNS pdt#3 DT</a>
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   <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/8/81/T--William_and_Mary--pdttag.jpeg" width=320px></div>
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  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333401">BBa_K2333401</a> </br></div>
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  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/2/23/T--William_and_Mary--pdttaga.jpeg" width=320px></div>
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  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3a tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333402">BBa_K2333402</a> </br></div>
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<a class="exp" href="#url" tabindex="1">UNS pdt#3b DT</a>
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  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/9/9b/T--William_and_Mary--pdttagb.jpeg" width=320px></div>
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  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3b tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333403">BBa_K2333403</a> </br></div>
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<!--UNS pdt#3c DT-->
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<a class="exp" href="#url" tabindex="1">UNS pdt#3c DT</a>
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  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/7/7b/T--William_and_Mary--pdttagc.jpeg" width=320px></div>
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  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3c tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333404">BBa_K2333404</a> </br></div>
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<!--UNS pdt#3d DT-->
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  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/c/c8/T--William_and_Mary--pdttagd.jpeg" width=320px></div>
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  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3d tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333405">BBa_K2333405</a> </br></div>
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  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/1/1f/T--William_and_Mary--pdttage.jpeg" width=320px></div>
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  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3e tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333406">BBa_K2333406</a> </br></div>
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<div class="container">

Revision as of 20:40, 5 July 2017

This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.

For a full list of all parts submitted to the iGEM registry, click here .