Team:William and Mary/Safety

Our Project

We worked on a fundamental project using E. coli (mostly cell line of 5-alpha and 10-beta) to investigate the effect of protein degradation on the speed of gene expression. Our basic method was co-transforming two plasmids, one that expresses sfGFP with synthetic degradation tags and another expresses mf- Lon protease, to E. Coli cell. The reporter protein concentrations correspond with the degradation strengths of those tags. We used plate reader measurements and time-lapse microscopy to characterize the degradation rates.

Safety and Training in the Lab

We are a Biosafety Level l lab, which means that none of the reagents are known to be pathogenic in healthy humans. We nevertheless followed all BSL-1 precautions, including wearing gloves at all times when working with BSL-1 agents, tightly controlling lab accessibility, appropriately inactivating infectious material through autoclaving and bleaching, banning all food and drink in the lab, and enforcing rigorous hand washing before leaving the lab to ensure the utmost safety. We received training from multiple sources. Before entering the lab, we received training from our PI in basic chemical safety and biosafety (BSL1). At the beginning of the summer, we also received a refresher course from our PI and reviewed BSL1 protocols extensively. In addition, at the beginning of the summer session, we received two hours of training on all aspects of lab safety from the Director of the Environmental Health and Safety Office, College of William and Mary. All training sessions were documented.