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− | <div id="pagebanner" style="background-image: url(https://static.igem.org/mediawiki/2017/ | + | <div id="pagebanner" style="background-image: url(https://static.igem.org/mediawiki/2017/8/80/TU_Dresden_S_Lab_Header.jpeg);"> |
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− | <div id="bannerquote"></div> | + | <div id="bannerquote" style="text-shadow: 0 0 3px var(--logo-dark-blue);">Fasten your seat belts!</div> |
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<main> | <main> | ||
<div class="contentbox"> | <div class="contentbox"> | ||
− | <h1 class="box-heading">Safety</h1> | + | <h1 class="box-heading">Safety expertise</h1> |
− | <figure style="width: | + | <figure> |
+ | <figure class="makeresponsive floatleft" style="width: 50%;"> | ||
<img src="https://static.igem.org/mediawiki/2017/f/f7/T--TU_Dresden--S_Lydiasafety.jpg" | <img src="https://static.igem.org/mediawiki/2017/f/f7/T--TU_Dresden--S_Lydiasafety.jpg" | ||
− | alt=" | + | alt="Safety talk"> |
− | <figcaption> | + | <figcaption>Legal assistant Ms K. Michalk from the Institute for Technological and Environmental Law at TU Dresden and team member Lydia Kirsche</figcaption></figure> |
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− | As our team aims at applying encapsulated bacteria in facilities other than our laboratories we had to consider the biological safety of our project. Therefore, we met with the legal assistant Ms K. Michalk from the Institute for Technological and Environmental Law at TU Dresden. She informed us about the procedure during the safety evaluation of a biological project and what is needed to make our Peptidosomes with the encapsulated biosensor applicable in e.g. a sewage treatment plant. According to her provisional assessment, the encapsulation of the biosensor into Peptidosomes represents a huge improvement in contrast to a biosensor that is placed on the market without further immobilization. She estimates this additional safety measure as pivotal for the potential prospective approval for a placement on the market. However, she also recommends the installation of further safety levels into our proposed portable system described below. Hereby, so-called indestructible materials could be suitable, preventing bacteria from escaping the system. | + | As our team aims at applying encapsulated bacteria in facilities other than our laboratories, we had to consider the biological safety of our project. Therefore, we met with the legal assistant Ms. K. Michalk from the Institute for Technological and Environmental Law at TU Dresden. She informed us about the procedure during the safety evaluation of a biological project and what is needed to make our Peptidosomes with the encapsulated biosensor applicable in e.g. a sewage treatment plant. According to her provisional assessment, the encapsulation of the biosensor into Peptidosomes represents a huge improvement in contrast to a biosensor that is placed on the market without further immobilization. She estimates this additional safety measure as pivotal for the potential prospective approval for a placement on the market. However, she also recommends the installation of further safety levels into our proposed portable system described below. Hereby, so-called indestructible materials could be suitable, preventing bacteria from escaping the system. </figure> |
+ | </div> | ||
<div class="contentbox"> | <div class="contentbox"> | ||
− | <h1 class="box-heading"> | + | <h1 class="box-heading">Risk assessment</h1> |
+ | |||
+ | <h2>Risk assessment of the Biosensor encapsulated in Peptidosomes</h2> | ||
<p> | <p> | ||
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The operon <i>luxABCDE</i> originates from <i>luminescens subsp. Luminescens.</i> This microorganism is non-pathogenic for humans.</p> | The operon <i>luxABCDE</i> originates from <i>luminescens subsp. Luminescens.</i> This microorganism is non-pathogenic for humans.</p> | ||
− | <p>Further a mutation has been introduced to the genome of W168, by exchanging the gene <i>penP</i> with a resistance cassette for kanamycin, causing a deletion of large parts of its coding sequence. This gene normally codes for a beta-lactamase, making Bacillus more resistant to several beta-lactam antibiotics. We deleted this gene | + | <p>Further a mutation has been introduced to the genome of W168, by exchanging the gene <i>penP</i> with a resistance cassette for kanamycin, causing a deletion of large parts of its coding sequence. This gene normally codes for a beta-lactamase, making Bacillus more resistant to several beta-lactam antibiotics. We deleted this gene to make the biosensor more sensitive to the antibiotic compounds and to ensure that the compounds of interest are not degraded by our strain. </p> |
<p>In summary, due to the introduction of the genetic constructs, the biosensor features resistant cassettes that facilitate resistance against Chloramphenicol (5mg/mL), MLS (1mg/mL Erythromycin; 25mg/mL Lincomycin), Kanamycin ((10mg/ml) and Spectinomycin (200mg/mL). </p> | <p>In summary, due to the introduction of the genetic constructs, the biosensor features resistant cassettes that facilitate resistance against Chloramphenicol (5mg/mL), MLS (1mg/mL Erythromycin; 25mg/mL Lincomycin), Kanamycin ((10mg/ml) and Spectinomycin (200mg/mL). </p> | ||
<p>Accorrding to § 1 Nr. 1 GenTG (Genetic engineering act) life and health of mankind, the environment and its interacting systems, fauna, flora and material assets must be protected from any harmful effects of the biosensor. | <p>Accorrding to § 1 Nr. 1 GenTG (Genetic engineering act) life and health of mankind, the environment and its interacting systems, fauna, flora and material assets must be protected from any harmful effects of the biosensor. | ||
− | Due to the detailed characterization of the genetically engineered organism, it is grouped into safety level 1 and therefore constitutes no risk. In the case of genetic engineering operations in a genetic engineering facility a notification is necessary. | + | Due to the detailed characterization of the genetically engineered organism, it is grouped into safety level 1 and therefore constitutes no risk. In the case of genetic engineering operations in a genetic engineering facility, a notification is necessary. |
This security assessment is supported by the required elements after § 7 Abs. 2 Nr. 1 GenTSV for the production section and § 7 Abs. 3 Nr. 1 GenTSV. </p> | This security assessment is supported by the required elements after § 7 Abs. 2 Nr. 1 GenTSV for the production section and § 7 Abs. 3 Nr. 1 GenTSV. </p> | ||
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<ol class="letters"> | <ol class="letters"> | ||
<li> Sewage treatment plants:</br> | <li> Sewage treatment plants:</br> | ||
− | The biosensor could be applied here to | + | The biosensor could be applied here to prove a potential contamination of the waste water with antibiotics.</li> |
<li> Food analysis:</br> | <li> Food analysis:</br> | ||
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There is the possibility to take water samples and transfer these into an S1-facility in which the test for antibiotic contamination using the biosensor is performed. Otherwise, we could think of an integration of the biosensor into the sewage treatment plant. Integration into a treatment plant would require reconstruction or renovations to the facility and demand a higher personnel requirement (compare to § 16 Nr. 1 GenTSV).</p> | There is the possibility to take water samples and transfer these into an S1-facility in which the test for antibiotic contamination using the biosensor is performed. Otherwise, we could think of an integration of the biosensor into the sewage treatment plant. Integration into a treatment plant would require reconstruction or renovations to the facility and demand a higher personnel requirement (compare to § 16 Nr. 1 GenTSV).</p> | ||
− | <p>The GMOs must be prevented from escaping the S1-facility of the sewage treatment plant at any time. To do so, a system needs to be installed | + | <p>The GMOs must be prevented from escaping the S1-facility of the sewage treatment plant at any time. To do so, a system needs to be installed that can guarantee that no living GMO leaves the facility. In contrast to the use of the biosensor without any matrix for encapsulation, Peptidosomes serve as an additional envelope or shell that keeps bacteria from breaking out of their cage. Due to this barrier, the maintenance of the GMOs in the S1-facility can be guaranteed. </p> |
<p>Furthermore, another visionary application in this sector constitutes a portable unit, which contains the encapsulated biosensor. This would enable the easy and fast examination of water samples in the field. This vision, in regard to application b), depicts a profitable improvement, precisely due to the easy transport of the biosensor and the low probability of occurrence of harmful effects. | <p>Furthermore, another visionary application in this sector constitutes a portable unit, which contains the encapsulated biosensor. This would enable the easy and fast examination of water samples in the field. This vision, in regard to application b), depicts a profitable improvement, precisely due to the easy transport of the biosensor and the low probability of occurrence of harmful effects. | ||
− | The | + | The extent of potentially harmful effects is marginal or even negligible. The construct of the biosensor represents an R1/S1-organism, which is not pathogenic for humans or harmful for the environment.</p> |
<p><b>In summary, it can be stated that the genetic engineering assessment of the Peptidosome as immobilization matrix depends solely on the encapsulated bacteria. The Peptidosome is a further safety precaution against the release of the microorganisms into the environment.</b></p> | <p><b>In summary, it can be stated that the genetic engineering assessment of the Peptidosome as immobilization matrix depends solely on the encapsulated bacteria. The Peptidosome is a further safety precaution against the release of the microorganisms into the environment.</b></p> |
Latest revision as of 22:47, 1 November 2017