Difference between revisions of "Team:Paris Bettencourt/Part Collection"
| Line 28: | Line 28: | ||
<table> | <table> | ||
<tr><th>Part Name</th><th>Part Type</th><th>Keyword</th><th>Designer</th><th>Length</th></tr> | <tr><th>Part Name</th><th>Part Type</th><th>Keyword</th><th>Designer</th><th>Length</th></tr> | ||
| − | <tr><td>BBa_K2510100</td><td>Coding</td><td></td><td> | + | <tr><td>BBa_K2510100</td><td>Coding</td><td>pdDronpa wt</td><td>Aya Gomaa</td><td>1490</td></tr> |
| − | <tr><td>BBa_K2510101</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510101</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510102</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510102</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510103</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510103</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510104</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510104</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510105</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510105</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510106</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510106</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510107</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510107</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510108</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510108</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510109</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510109</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510110</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510110</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510110</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510110</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510111</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510111</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510112</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510112</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510113</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510113</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
| − | <tr><td>BBa_K2510018</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510018</td><td>Coding</td><td></td><td>Alma Chapet--Batlle</td><td></td></tr> |
| − | <tr><td>BBa_K2510019</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510019</td><td>Coding</td><td></td><td>Alma Chapet--Batlle</td><td></td></tr> |
| − | <tr><td>BBa_K2510020</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510020</td><td>Coding</td><td></td><td>Alma Chapet--Batlle</td><td></td></tr> |
| − | <tr><td>BBa_K2510021</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510021</td><td>Coding</td><td></td><td>Alma Chapet--Batlle</td><td></td></tr> |
| − | <tr><td>BBa_K2510022</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510022</td><td>Coding</td><td></td><td>Alma Chapet--Batlle</td><td></td></tr> |
| − | <tr><td>BBa_K2510024</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510024</td><td>Coding</td><td></td><td>Alma Chapet--Batlle</td><td></td></tr> |
| − | <tr><td>BBa_K2510025</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510025</td><td>Coding</td><td></td><td>Alma Chapet--Batlle</td><td></td></tr> |
| − | <tr><td>BBa_K2510026</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510026</td><td>Coding</td><td></td><td>Alma Chapet--Batlle</td><td></td></tr> |
| − | <tr><td>BBa_K2510028</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510028</td><td>Coding</td><td></td><td>Alma Chapet--Batlle</td><td></td></tr> |
| − | <tr><td>BBa_K2510029</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K2510029</td><td>Coding</td><td></td><td>Alma Chapet--Batlle</td><td></td></tr> |
</table> | </table> | ||
Revision as of 01:13, 2 November 2017
PART COLLECTION
Part Collection
The parts in this collection were designed to form either AND or NOR gates depending on light inputs. Photo-switchable proteins that alternate between homodimer and monomer conformations under specific wavelengths were designed to flank a protein of interest. This creates a protein cage, sterically repressing activity when no light is applied. Inserting either the T7 RNA polymerase or one of three repressors - TetR, HKcI and P22c2 (Parts BBa_K2510100 to BBa_K2510113) - gave us a number of photo-switchable transcription factors. To create logic, we combined such proteins with our library of 12 dually repressible promoters (Parts: BBa_K2510018 to BBa_K2510029), consisting of a single T7 promoter core and two synthetic orthogonal operators. For the first time, we are able to reversibly control gene expression at a high spatio-temporal resolution with easy procedures and minimal cost to the cell.
| Part Name | Part Type | Keyword | Designer | Length |
|---|---|---|---|---|
| BBa_K2510100 | Coding | pdDronpa wt | Aya Gomaa | 1490 |
| BBa_K2510101 | Coding | Aya Gomaa | ||
| BBa_K2510102 | Coding | Aya Gomaa | ||
| BBa_K2510103 | Coding | Aya Gomaa | ||
| BBa_K2510104 | Coding | Aya Gomaa | ||
| BBa_K2510105 | Coding | Aya Gomaa | ||
| BBa_K2510106 | Coding | Aya Gomaa | ||
| BBa_K2510107 | Coding | Aya Gomaa | ||
| BBa_K2510108 | Coding | Aya Gomaa | ||
| BBa_K2510109 | Coding | Aya Gomaa | ||
| BBa_K2510110 | Coding | Aya Gomaa | ||
| BBa_K2510110 | Coding | Aya Gomaa | ||
| BBa_K2510111 | Coding | Aya Gomaa | ||
| BBa_K2510112 | Coding | Aya Gomaa | ||
| BBa_K2510113 | Coding | Aya Gomaa | ||
| BBa_K2510018 | Coding | Alma Chapet--Batlle | ||
| BBa_K2510019 | Coding | Alma Chapet--Batlle | ||
| BBa_K2510020 | Coding | Alma Chapet--Batlle | ||
| BBa_K2510021 | Coding | Alma Chapet--Batlle | ||
| BBa_K2510022 | Coding | Alma Chapet--Batlle | ||
| BBa_K2510024 | Coding | Alma Chapet--Batlle | ||
| BBa_K2510025 | Coding | Alma Chapet--Batlle | ||
| BBa_K2510026 | Coding | Alma Chapet--Batlle | ||
| BBa_K2510028 | Coding | Alma Chapet--Batlle | ||
| BBa_K2510029 | Coding | Alma Chapet--Batlle |
Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
bettencourt.igem2017@gmail.com
bettencourt.igem2017@gmail.com 
