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<tr><td>BBa_K2510102</td><td>Coding</td><td>pdDronpa mutant </td><td>Aya Gomaa</td><td>1490</td></tr> | <tr><td>BBa_K2510102</td><td>Coding</td><td>pdDronpa mutant </td><td>Aya Gomaa</td><td>1490</td></tr> | ||
<tr><td>BBa_K2510103</td><td>Coding</td><td>lacz-pdDronpa</td><td>Aya Gomaa</td><td>4584</td></tr> | <tr><td>BBa_K2510103</td><td>Coding</td><td>lacz-pdDronpa</td><td>Aya Gomaa</td><td>4584</td></tr> | ||
− | <tr><td>BBa_K2510104</td><td>Coding</td><td>T7pol-pdDronpa wt under Anderson promoter </td><td>Aya Gomaa</td><td></td></tr> | + | <tr><td>BBa_K2510104</td><td>Coding</td><td>T7pol-pdDronpa wt under Anderson promoter </td><td>Aya Gomaa</td><td>4161</td></tr> |
− | <tr><td>BBa_K2510105</td><td>Coding</td><td></td><td>Aya Gomaa</td><td> | + | <tr><td>BBa_K2510105</td><td>Coding</td><td>T7pol-pdDronpa wt under t7 promoter</td><td>Aya Gomaa</td><td>4210</td></tr> |
− | <tr><td>BBa_K2510106</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> | + | <tr><td>BBa_K2510106</td><td>Coding</td><td>T7pol-pdDronpa mutant under t7 promoter</td><td>Aya Gomaa</td><td>4161</td></tr> |
− | <tr><td>BBa_K2510107</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> | + | <tr><td>BBa_K2510107</td><td>Coding</td><td>mut Dronpa t7 pol phusion + t7 mrfp part</td><td>Aya Gomaa</td><td>5063</td></tr> |
<tr><td>BBa_K2510108</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> | <tr><td>BBa_K2510108</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> | ||
<tr><td>BBa_K2510109</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> | <tr><td>BBa_K2510109</td><td>Coding</td><td></td><td>Aya Gomaa</td><td></td></tr> |
Revision as of 01:22, 2 November 2017
![](https://static.igem.org/mediawiki/2017/e/e2/Arrow2PB.png)
PART COLLECTION
Part Collection
The parts in this collection were designed to form either AND or NOR gates depending on light inputs. Photo-switchable proteins that alternate between homodimer and monomer conformations under specific wavelengths were designed to flank a protein of interest. This creates a protein cage, sterically repressing activity when no light is applied. Inserting either the T7 RNA polymerase or one of three repressors - TetR, HKcI and P22c2 (Parts BBa_K2510100 to BBa_K2510113) - gave us a number of photo-switchable transcription factors. To create logic, we combined such proteins with our library of 12 dually repressible promoters (Parts: BBa_K2510018 to BBa_K2510029), consisting of a single T7 promoter core and two synthetic orthogonal operators. For the first time, we are able to reversibly control gene expression at a high spatio-temporal resolution with easy procedures and minimal cost to the cell.
Part Name | Part Type | Keyword | Designer | Length |
---|---|---|---|---|
BBa_K2510100 | Coding | pdDronpa wt | Aya Gomaa | 1490 |
BBa_K2510101 | Coding | PDDronpa wt t7 promoter | Aya Gomaa | 1540 |
BBa_K2510102 | Coding | pdDronpa mutant | Aya Gomaa | 1490 |
BBa_K2510103 | Coding | lacz-pdDronpa | Aya Gomaa | 4584 |
BBa_K2510104 | Coding | T7pol-pdDronpa wt under Anderson promoter | Aya Gomaa | 4161 |
BBa_K2510105 | Coding | T7pol-pdDronpa wt under t7 promoter | Aya Gomaa | 4210 |
BBa_K2510106 | Coding | T7pol-pdDronpa mutant under t7 promoter | Aya Gomaa | 4161 |
BBa_K2510107 | Coding | mut Dronpa t7 pol phusion + t7 mrfp part | Aya Gomaa | 5063 |
BBa_K2510108 | Coding | Aya Gomaa | ||
BBa_K2510109 | Coding | Aya Gomaa | ||
BBa_K2510110 | Coding | Aya Gomaa | ||
BBa_K2510110 | Coding | Aya Gomaa | ||
BBa_K2510111 | Coding | Aya Gomaa | ||
BBa_K2510112 | Coding | Aya Gomaa | ||
BBa_K2510113 | Coding | Aya Gomaa | ||
BBa_K2510018 | Coding | Alma Chapet--Batlle | ||
BBa_K2510019 | Coding | Alma Chapet--Batlle | ||
BBa_K2510020 | Coding | Alma Chapet--Batlle | ||
BBa_K2510021 | Coding | Alma Chapet--Batlle | ||
BBa_K2510022 | Coding | Alma Chapet--Batlle | ||
BBa_K2510024 | Coding | Alma Chapet--Batlle | ||
BBa_K2510025 | Coding | Alma Chapet--Batlle | ||
BBa_K2510026 | Coding | Alma Chapet--Batlle | ||
BBa_K2510028 | Coding | Alma Chapet--Batlle | ||
BBa_K2510029 | Coding | Alma Chapet--Batlle |