Difference between revisions of "Team:Paris Bettencourt/Part Collection"

 
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<div id="header"> Part Collection </div>
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<div id=header1 class="header">PART COLLECTION</div>
  
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1> Part Collection </h1>
 
<h1> Part Collection </h1>
  
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<div class=text1>The parts in this collection were designed to form either AND or NOR gates depending on light inputs. Photo-switchable proteins that alternate between homodimer and monomer conformations under specific wavelengths were designed to flank a protein of interest. This creates a protein cage, sterically repressing activity when no light is applied. Inserting either the T7 RNA polymerase or one of three repressors - TetR, HKcI and P22c2 (Parts BBa_K2510100 to BBa_K2510113) - gave us a number of photo-switchable transcription factors. To create logic, we combined such proteins with our library of 12 dually repressible promoters (Parts: BBa_K2510018 to BBa_K2510029), consisting of a single T7 promoter core and two synthetic orthogonal operators. For the first time, we are able to reversibly control gene expression at a high spatio-temporal resolution with easy procedures and minimal cost to the cell. </div></br></br>
  
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
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    <tr><th>Part Name</th><th>Part Type</th><th>Keyword</th><th>Designer</th><th>Length</th></tr>
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    <tr><td>BBa_K2510100</td><td>Coding</td><td>pdDronpa wt</td><td>Aya Gomaa</td><td>1490</td></tr>
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While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.
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    <tr><td>BBa_K2510101</td><td>Coding</td><td>PDDronpa wt t7 promoter </td><td>Aya Gomaa</td><td>1540</td></tr>
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    <tr><td>BBa_K2510102</td><td>Coding</td><td>pdDronpa mutant </td><td>Aya Gomaa</td><td>1490</td></tr>
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    <tr><td>BBa_K2510103</td><td>Coding</td><td>lacz-pdDronpa</td><td>Aya Gomaa</td><td>4584</td></tr>
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<h3>Best Part Collection Special Prize</h3>
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    <tr><td>BBa_K2510104</td><td>Coding</td><td>T7pol-pdDronpa wt under Anderson promoter </td><td>Aya Gomaa</td><td>4161</td></tr>
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<p>Did your team make a lot of great parts? Is there a team that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection or a collection of awesome pigment parts? Tell the judges you should be evaluated for the Best Parts Collection award! To be eligible for this award, these parts must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts.
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    <tr><td>BBa_K2510105</td><td>Coding</td><td>T7pol-pdDronpa wt under t7 promoter</td><td>Aya Gomaa</td><td>4210</td></tr>
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    <tr><td>BBa_K2510106</td><td>Coding</td><td>T7pol-pdDronpa mutant under t7 promoter</td><td>Aya Gomaa</td><td>4161</td></tr>
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If you have a collection of parts you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part numbers to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.</p>
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    <tr><td>BBa_K2510107</td><td>Coding</td><td>mut Dronpa t7 pol phusion + t7 mrfp part</td><td>Aya Gomaa</td><td>5063</td></tr>
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    <tr><td>BBa_K2510108</td><td>Coding</td><td>pdDronpa wt TetR</td><td>Aya Gomaa</td><td>2182</td></tr>
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    <tr><td>BBa_K2510109</td><td>Coding</td><td>pdDronpa mut TetR</td><td>Aya Gomaa</td><td>2133</td></tr>
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    <tr><td>BBa_K2510110</td><td>Coding</td><td>pdDronpa wt HKCI</td><td>Aya Gomaa</td><td>2266</td></tr>
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    <tr><td>BBa_K2510111</td><td>Coding</td><td>pdDronpa mut HKCI</td><td>Aya Gomaa</td><td>2217</td></tr>
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    <tr><td>BBa_K2510112</td><td>Coding</td><td>pdDronpa wt P22C2</td><td>Aya Gomaa</td><td>2242</td></tr>
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<h4>Note</h4>
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    <tr><td>BBa_K2510113</td><td>Coding</td><td>pdDronpa mut P22c2</td><td>Aya Gomaa</td><td>2193</td></tr>
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<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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    <tr><td>BBa_K2510018</td><td>Regulatory</td><td>T7 - O-HKcI - O-P22c2 promoter</td><td>Alma Chapet--Batlle</td><td>74</td></tr>
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    <tr><td>BBa_K2510019</td><td>Regulatory</td><td>T7 - O-HKcI - O-TetR promoter</td><td>Alma Chapet--Batlle</td><td>74</td></tr>
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    <tr><td>BBa_K2510020</td><td>Regulatory</td><td>T7 - O-P22c2 - O-HKcI promoter</td><td>Alma Chapet--Batlle</td><td>74</td></tr>
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    <tr><td>BBa_K2510021</td><td>Regulatory</td><td>T7 - O-P22c2 - O-TetR promoter</td><td>Alma Chapet--Batlle</td><td>84</td></tr>
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    <tr><td>BBa_K2510022</td><td>Regulatory</td><td>T7- O-TetR - O-HKcI promoter</td><td>Alma Chapet--Batlle</td><td>74</td></tr>
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    <tr><td>BBa_K2510024</td><td>Regulatory</td><td>O-HKcI - T7 - O-HKcI - O-P22c2 promoter</td><td>Alma Chapet--Batlle</td><td>95</td></tr>
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    <tr><td>BBa_K2510025</td><td>Regulatory</td><td>O-HKcI - T7 - O-HKcI - O-TetR promoter</td><td>Alma Chapet--Batlle</td><td>95</td></tr>
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    <tr><td>BBa_K2510026</td><td>Regulatory</td><td>O-P22C2 - T7 - O-P22C2 - O-HKcI promoter</td><td>Alma Chapet--Batlle</td><td>107</td></tr>
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    <tr><td>BBa_K2510028</td><td>Regulatory</td><td>O-TetR - T7 - O-TetR - O-HKcI promoter</td><td>Alma Chapet--Batlle</td><td>115</td></tr>
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    <tr><td>BBa_K2510029</td><td>Regulatory</td><td>O-TetR - T7 - O-TetR - O-P22C2 promoter</td><td>Alma Chapet--Batlle</td><td>125</td></tr>
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Latest revision as of 02:25, 2 November 2017

PART COLLECTION

Part Collection

The parts in this collection were designed to form either AND or NOR gates depending on light inputs. Photo-switchable proteins that alternate between homodimer and monomer conformations under specific wavelengths were designed to flank a protein of interest. This creates a protein cage, sterically repressing activity when no light is applied. Inserting either the T7 RNA polymerase or one of three repressors - TetR, HKcI and P22c2 (Parts BBa_K2510100 to BBa_K2510113) - gave us a number of photo-switchable transcription factors. To create logic, we combined such proteins with our library of 12 dually repressible promoters (Parts: BBa_K2510018 to BBa_K2510029), consisting of a single T7 promoter core and two synthetic orthogonal operators. For the first time, we are able to reversibly control gene expression at a high spatio-temporal resolution with easy procedures and minimal cost to the cell.


Part NamePart TypeKeywordDesignerLength
BBa_K2510100CodingpdDronpa wtAya Gomaa1490
BBa_K2510101CodingPDDronpa wt t7 promoter Aya Gomaa1540
BBa_K2510102CodingpdDronpa mutant Aya Gomaa1490
BBa_K2510103Codinglacz-pdDronpaAya Gomaa4584
BBa_K2510104CodingT7pol-pdDronpa wt under Anderson promoter Aya Gomaa4161
BBa_K2510105CodingT7pol-pdDronpa wt under t7 promoterAya Gomaa4210
BBa_K2510106CodingT7pol-pdDronpa mutant under t7 promoterAya Gomaa4161
BBa_K2510107Codingmut Dronpa t7 pol phusion + t7 mrfp partAya Gomaa5063
BBa_K2510108CodingpdDronpa wt TetRAya Gomaa2182
BBa_K2510109CodingpdDronpa mut TetRAya Gomaa2133
BBa_K2510110CodingpdDronpa wt HKCIAya Gomaa2266
BBa_K2510111CodingpdDronpa mut HKCIAya Gomaa2217
BBa_K2510112CodingpdDronpa wt P22C2Aya Gomaa2242
BBa_K2510113CodingpdDronpa mut P22c2Aya Gomaa2193
BBa_K2510018RegulatoryT7 - O-HKcI - O-P22c2 promoterAlma Chapet--Batlle74
BBa_K2510019RegulatoryT7 - O-HKcI - O-TetR promoterAlma Chapet--Batlle74
BBa_K2510020RegulatoryT7 - O-P22c2 - O-HKcI promoterAlma Chapet--Batlle74
BBa_K2510021RegulatoryT7 - O-P22c2 - O-TetR promoterAlma Chapet--Batlle84
BBa_K2510022RegulatoryT7- O-TetR - O-HKcI promoterAlma Chapet--Batlle74
BBa_K2510024RegulatoryO-HKcI - T7 - O-HKcI - O-P22c2 promoterAlma Chapet--Batlle95
BBa_K2510025RegulatoryO-HKcI - T7 - O-HKcI - O-TetR promoterAlma Chapet--Batlle95
BBa_K2510026RegulatoryO-P22C2 - T7 - O-P22C2 - O-HKcI promoterAlma Chapet--Batlle107
BBa_K2510028RegulatoryO-TetR - T7 - O-TetR - O-HKcI promoterAlma Chapet--Batlle115
BBa_K2510029RegulatoryO-TetR - T7 - O-TetR - O-P22C2 promoterAlma Chapet--Batlle125

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
bettencourt.igem2017@gmail.com