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<h1> Part Collection </h1> | <h1> Part Collection </h1> | ||
− | <div class=text1>The parts in this collection were designed to form either AND or NOR gates depending on light inputs. Photo-switchable proteins that alternate between homodimer and monomer conformations under specific wavelengths were designed to flank a protein of interest. This creates a protein cage, sterically repressing activity when no light is applied. Inserting either the T7 RNA polymerase or one of three repressors - TetR, HKcI and P22c2 (Parts BBa_K2510100 to BBa_K2510113) - gave us a number of photo-switchable transcription factors. To create logic, we combined such proteins with our library of 12 dually repressible promoters (Parts: BBa_K2510018 to BBa_K2510029), consisting of a single T7 promoter core and two synthetic orthogonal operators. For the first time, we are able to reversibly control gene expression at a high spatio-temporal resolution with easy procedures and minimal cost to the cell. </div> | + | <div class=text1>The parts in this collection were designed to form either AND or NOR gates depending on light inputs. Photo-switchable proteins that alternate between homodimer and monomer conformations under specific wavelengths were designed to flank a protein of interest. This creates a protein cage, sterically repressing activity when no light is applied. Inserting either the T7 RNA polymerase or one of three repressors - TetR, HKcI and P22c2 (Parts BBa_K2510100 to BBa_K2510113) - gave us a number of photo-switchable transcription factors. To create logic, we combined such proteins with our library of 12 dually repressible promoters (Parts: BBa_K2510018 to BBa_K2510029), consisting of a single T7 promoter core and two synthetic orthogonal operators. For the first time, we are able to reversibly control gene expression at a high spatio-temporal resolution with easy procedures and minimal cost to the cell. </div></br></br> |
+ | |||
+ | <table> | ||
+ | <tr><th>Part Name</th><th>Part Type</th><th>Keyword</th><th>Designer</th><th>Length</th></tr> | ||
+ | <tr><td>BBa_K2510100</td><td>Coding</td><td>pdDronpa wt</td><td>Aya Gomaa</td><td>1490</td></tr> | ||
+ | <tr><td>BBa_K2510101</td><td>Coding</td><td>PDDronpa wt t7 promoter </td><td>Aya Gomaa</td><td>1540</td></tr> | ||
+ | <tr><td>BBa_K2510102</td><td>Coding</td><td>pdDronpa mutant </td><td>Aya Gomaa</td><td>1490</td></tr> | ||
+ | <tr><td>BBa_K2510103</td><td>Coding</td><td>lacz-pdDronpa</td><td>Aya Gomaa</td><td>4584</td></tr> | ||
+ | <tr><td>BBa_K2510104</td><td>Coding</td><td>T7pol-pdDronpa wt under Anderson promoter </td><td>Aya Gomaa</td><td>4161</td></tr> | ||
+ | <tr><td>BBa_K2510105</td><td>Coding</td><td>T7pol-pdDronpa wt under t7 promoter</td><td>Aya Gomaa</td><td>4210</td></tr> | ||
+ | <tr><td>BBa_K2510106</td><td>Coding</td><td>T7pol-pdDronpa mutant under t7 promoter</td><td>Aya Gomaa</td><td>4161</td></tr> | ||
+ | <tr><td>BBa_K2510107</td><td>Coding</td><td>mut Dronpa t7 pol phusion + t7 mrfp part</td><td>Aya Gomaa</td><td>5063</td></tr> | ||
+ | <tr><td>BBa_K2510108</td><td>Coding</td><td>pdDronpa wt TetR</td><td>Aya Gomaa</td><td>2182</td></tr> | ||
+ | <tr><td>BBa_K2510109</td><td>Coding</td><td>pdDronpa mut TetR</td><td>Aya Gomaa</td><td>2133</td></tr> | ||
+ | <tr><td>BBa_K2510110</td><td>Coding</td><td>pdDronpa wt HKCI</td><td>Aya Gomaa</td><td>2266</td></tr> | ||
+ | <tr><td>BBa_K2510111</td><td>Coding</td><td>pdDronpa mut HKCI</td><td>Aya Gomaa</td><td>2217</td></tr> | ||
+ | <tr><td>BBa_K2510112</td><td>Coding</td><td>pdDronpa wt P22C2</td><td>Aya Gomaa</td><td>2242</td></tr> | ||
+ | <tr><td>BBa_K2510113</td><td>Coding</td><td>pdDronpa mut P22c2</td><td>Aya Gomaa</td><td>2193</td></tr> | ||
+ | <tr><td>BBa_K2510018</td><td>Regulatory</td><td>T7 - O-HKcI - O-P22c2 promoter</td><td>Alma Chapet--Batlle</td><td>74</td></tr> | ||
+ | <tr><td>BBa_K2510019</td><td>Regulatory</td><td>T7 - O-HKcI - O-TetR promoter</td><td>Alma Chapet--Batlle</td><td>74</td></tr> | ||
+ | <tr><td>BBa_K2510020</td><td>Regulatory</td><td>T7 - O-P22c2 - O-HKcI promoter</td><td>Alma Chapet--Batlle</td><td>74</td></tr> | ||
+ | <tr><td>BBa_K2510021</td><td>Regulatory</td><td>T7 - O-P22c2 - O-TetR promoter</td><td>Alma Chapet--Batlle</td><td>84</td></tr> | ||
+ | <tr><td>BBa_K2510022</td><td>Regulatory</td><td>T7- O-TetR - O-HKcI promoter</td><td>Alma Chapet--Batlle</td><td>74</td></tr> | ||
+ | <tr><td>BBa_K2510024</td><td>Regulatory</td><td>O-HKcI - T7 - O-HKcI - O-P22c2 promoter</td><td>Alma Chapet--Batlle</td><td>95</td></tr> | ||
+ | <tr><td>BBa_K2510025</td><td>Regulatory</td><td>O-HKcI - T7 - O-HKcI - O-TetR promoter</td><td>Alma Chapet--Batlle</td><td>95</td></tr> | ||
+ | <tr><td>BBa_K2510026</td><td>Regulatory</td><td>O-P22C2 - T7 - O-P22C2 - O-HKcI promoter</td><td>Alma Chapet--Batlle</td><td>107</td></tr> | ||
+ | <tr><td>BBa_K2510028</td><td>Regulatory</td><td>O-TetR - T7 - O-TetR - O-HKcI promoter</td><td>Alma Chapet--Batlle</td><td>115</td></tr> | ||
+ | <tr><td>BBa_K2510029</td><td>Regulatory</td><td>O-TetR - T7 - O-TetR - O-P22C2 promoter</td><td>Alma Chapet--Batlle</td><td>125</td></tr> | ||
+ | </table> | ||
</div> | </div> |
Latest revision as of 02:25, 2 November 2017
PART COLLECTION
Part Collection
The parts in this collection were designed to form either AND or NOR gates depending on light inputs. Photo-switchable proteins that alternate between homodimer and monomer conformations under specific wavelengths were designed to flank a protein of interest. This creates a protein cage, sterically repressing activity when no light is applied. Inserting either the T7 RNA polymerase or one of three repressors - TetR, HKcI and P22c2 (Parts BBa_K2510100 to BBa_K2510113) - gave us a number of photo-switchable transcription factors. To create logic, we combined such proteins with our library of 12 dually repressible promoters (Parts: BBa_K2510018 to BBa_K2510029), consisting of a single T7 promoter core and two synthetic orthogonal operators. For the first time, we are able to reversibly control gene expression at a high spatio-temporal resolution with easy procedures and minimal cost to the cell.
Part Name | Part Type | Keyword | Designer | Length |
---|---|---|---|---|
BBa_K2510100 | Coding | pdDronpa wt | Aya Gomaa | 1490 |
BBa_K2510101 | Coding | PDDronpa wt t7 promoter | Aya Gomaa | 1540 |
BBa_K2510102 | Coding | pdDronpa mutant | Aya Gomaa | 1490 |
BBa_K2510103 | Coding | lacz-pdDronpa | Aya Gomaa | 4584 |
BBa_K2510104 | Coding | T7pol-pdDronpa wt under Anderson promoter | Aya Gomaa | 4161 |
BBa_K2510105 | Coding | T7pol-pdDronpa wt under t7 promoter | Aya Gomaa | 4210 |
BBa_K2510106 | Coding | T7pol-pdDronpa mutant under t7 promoter | Aya Gomaa | 4161 |
BBa_K2510107 | Coding | mut Dronpa t7 pol phusion + t7 mrfp part | Aya Gomaa | 5063 |
BBa_K2510108 | Coding | pdDronpa wt TetR | Aya Gomaa | 2182 |
BBa_K2510109 | Coding | pdDronpa mut TetR | Aya Gomaa | 2133 |
BBa_K2510110 | Coding | pdDronpa wt HKCI | Aya Gomaa | 2266 |
BBa_K2510111 | Coding | pdDronpa mut HKCI | Aya Gomaa | 2217 |
BBa_K2510112 | Coding | pdDronpa wt P22C2 | Aya Gomaa | 2242 |
BBa_K2510113 | Coding | pdDronpa mut P22c2 | Aya Gomaa | 2193 |
BBa_K2510018 | Regulatory | T7 - O-HKcI - O-P22c2 promoter | Alma Chapet--Batlle | 74 |
BBa_K2510019 | Regulatory | T7 - O-HKcI - O-TetR promoter | Alma Chapet--Batlle | 74 |
BBa_K2510020 | Regulatory | T7 - O-P22c2 - O-HKcI promoter | Alma Chapet--Batlle | 74 |
BBa_K2510021 | Regulatory | T7 - O-P22c2 - O-TetR promoter | Alma Chapet--Batlle | 84 |
BBa_K2510022 | Regulatory | T7- O-TetR - O-HKcI promoter | Alma Chapet--Batlle | 74 |
BBa_K2510024 | Regulatory | O-HKcI - T7 - O-HKcI - O-P22c2 promoter | Alma Chapet--Batlle | 95 |
BBa_K2510025 | Regulatory | O-HKcI - T7 - O-HKcI - O-TetR promoter | Alma Chapet--Batlle | 95 |
BBa_K2510026 | Regulatory | O-P22C2 - T7 - O-P22C2 - O-HKcI promoter | Alma Chapet--Batlle | 107 |
BBa_K2510028 | Regulatory | O-TetR - T7 - O-TetR - O-HKcI promoter | Alma Chapet--Batlle | 115 |
BBa_K2510029 | Regulatory | O-TetR - T7 - O-TetR - O-P22C2 promoter | Alma Chapet--Batlle | 125 |