Difference between revisions of "Team:AQA Unesp"

 
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.foto-grande {
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<img src="https://2017.igem.org/File:T--AQA_Unesp--logoInsubiota.png">
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background: url(https://static.igem.org/mediawiki/2017/6/68/Aqaunesp-main-header.png) no-repeat center center;
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    src: url('https://static.igem.org/mediawiki/2017/4/43/Aqaunesp-caviardreams.ttf');
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.insubiota-text {  font:12em caviardreams, sans-serif; text-align: center; color:white }
  
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.insubiota-subtext {  font:2em caviardreams, sans-serif; text-align: center; color:white; }
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<h1> Welcome to iGEM 2017! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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#abstract { font:5em caviardreams, sans-serif; text-align: center; color: white; font-weight:bold; padding-top: 2%}
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<img src="https://static.igem.org/mediawiki/2017/e/ec/Gold-award-aqaunesp.png" width="200px" id="gold-award">
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<div class="insubiota-text">INSUBIOTA</div>
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<div class="insubiota-subtext">treating diabetes with genetically engineered probiotic</div>
  
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<h5>Before you start: </h5>
 
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<p> Please read the following pages:</p>
 
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<ul>
 
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
 
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
 
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
 
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</ul>
 
 
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<div style="width:100%; margin: 0 auto; background-color: #44558f">
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<div id="abstract">ABSTRACT</div>
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<div style="max-width:1000px; font-size:1.5em; margin: 2% auto; line-height:1.5; text-align:justify; color: white; padding-top:2%; padding-bottom: 2%">
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Our project was inspired on the alarming and increasing number of diabetic people, especially diabetes mellitus type 1 patients, who are insulin dependent. The lack of less invasive treatments has motivated us to develop a new treatment based on the probiotic bacteria <i>Lactococcus lactis</i>, that was engineered to produce a single-chain analog insulin in human diabetic’s microbiota. The bacteria will be able to produce the insulin associated with a secretion signal sequence and cell-penetrating peptides, to ensure its uptake. Moreover, the synthesis of the insulin will be controlled by the natural bacteria system of catabolite repression with regulation by a small RNA. At the presence of glucose, the insulin gene expression will be activated, and then, it will be ready to be secreted and absorbed, reaching the blood and performing its biological function. Also, in order to contain the release of our engineered probiotic to the environment, we built a kill switch based on light exposure that uses the CRISPR/Cas9 system to destroy essential genes for the bacterial survival. The final product could be a fermented milk or a lyophilized that could be easily ingested by patients.
  
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<div class="highlight">
 
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<h5> Styling your wiki </h5>
 
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 
 
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<div style="text-align:center">
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<h5> Wiki template information </h5>
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<video width="1000" controls>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<source src="https://static.igem.org/mediawiki/2017/a/a7/Aqaunesp-video-insubiota.mp4" type="video/mp4">
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#buttons { max-width:1100px; margin:3% auto; display:table}
  
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.botao-link {
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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  top: 0;
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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  bottom: 0;
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  left: 0;
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border-radius:50%;
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#projectdesc {background-color: RGB(255,208,91)}
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#design {background-color: RGB(159,159,226)}
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#hpp {background-color: RGB(50,74,94)}
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#team {background-color: RGB(255,76,58)}
  
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<h5>Tips</h5>
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  color: white;
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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  font-size: 20px;
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<ul>
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  position: absolute;
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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  top: 50%;
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<li>Be clear about what you are doing and how you plan to do this.</li>
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  left: 50%;
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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  transform: translate(-50%, -50%);
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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  -ms-transform: translate(-50%, -50%);
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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}
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>
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<div id="buttons">
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<div class="botao-link">
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<ul>
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<a href="https://2017.igem.org/Team:AQA_Unesp/Description">
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<img src="https://static.igem.org/mediawiki/2017/f/f5/Aqaunesp-projectdescription-icon.png" class="image">
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<div class="overlay" id="projectdesc">
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<div class="text">project description</div>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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</div>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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</a>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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</div>
 
</div>
  
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<div class="botao-link">
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<h5> Uploading pictures and files </h5>
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<a href="https://2017.igem.org/Team:AQA_Unesp/Applied_Design">
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<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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<img src="https://static.igem.org/mediawiki/2017/c/cd/AQAUnesp-design-icon.png" class="image">
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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<div class="overlay" id="design">
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<div class="text">design</div>
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</div>
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</a>
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</div>
  
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<a href="https://2017.igem.org/Special:Upload">
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<div class="botao-link">
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UPLOAD FILES
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<a href="https://2017.igem.org/Team:AQA_Unesp/Results">
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<img src="https://static.igem.org/mediawiki/2017/7/7c/Aqaunesp-results-icon.png" class="image">
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<div class="overlay" id="projectdesc">
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<div class="text">results</div>
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</div>
 
</a>
 
</a>
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</p>
 
 
</div>
 
</div>
  
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<div class="botao-link">
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<a href="https://2017.igem.org/Team:AQA_Unesp/HP/Silver">
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<img src="https://static.igem.org/mediawiki/2017/0/0a/AQAUnesp-hp-icon.png" class="image">
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<div class="overlay" id="hpp">
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<div class="text">human practices</div>
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</div>
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</a>
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</div>
  
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<div class="botao-link">
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<a href="https://2017.igem.org/Team:AQA_Unesp/Team">
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<img src="https://static.igem.org/mediawiki/2017/b/bd/AQAUnesp-team-icon.png" class="image">
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<div class="overlay" id="team">
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<div class="text">team</div>
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</div>
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</a>
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{{AQA_Unesp_btm}}

Latest revision as of 18:13, 15 December 2017



iGEM AQA_Unesp

INSUBIOTA
treating diabetes with genetically engineered probiotic
ABSTRACT
Our project was inspired on the alarming and increasing number of diabetic people, especially diabetes mellitus type 1 patients, who are insulin dependent. The lack of less invasive treatments has motivated us to develop a new treatment based on the probiotic bacteria Lactococcus lactis, that was engineered to produce a single-chain analog insulin in human diabetic’s microbiota. The bacteria will be able to produce the insulin associated with a secretion signal sequence and cell-penetrating peptides, to ensure its uptake. Moreover, the synthesis of the insulin will be controlled by the natural bacteria system of catabolite repression with regulation by a small RNA. At the presence of glucose, the insulin gene expression will be activated, and then, it will be ready to be secreted and absorbed, reaching the blood and performing its biological function. Also, in order to contain the release of our engineered probiotic to the environment, we built a kill switch based on light exposure that uses the CRISPR/Cas9 system to destroy essential genes for the bacterial survival. The final product could be a fermented milk or a lyophilized that could be easily ingested by patients.
Team: AQA_Unesp