Difference between revisions of "Team:William and Mary/Model"

One of our major goals in developing the pdt speed-control system was to allow future teams to obtain control over the dynamical properties of their circuits through modifications that they make at the level of a single genetic part. As a proof-of-concept demonstration of this capability, we construct an Incoherent Feedforward Loop (IFFL) circuit whose dynamical properties are controlled by Lon activity. We demonstrate that we can predictably tune the sharpness of the circuit’s pulsatile response simply by swapping the choice of pdt.

One of the simplest examples of a dynamical circuit is the incoherent feed forward loop (IFFL), which consists of three proteins X, Y, and Z which regulate each other such that X activates Y and Z, and Y represses Z. This circuit architecture can generate a pulsatile response upon activation of X (Figure 1).