Difference between revisions of "Team:Paris Bettencourt"

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<header><div id=name>iGEM Paris-Bettencourt 2017</div></header>
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<div id=abstract><b>Medusa: Bringing control to the third dimension</b></br></div>
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<div id=abstract>Accurate spatial-temporal response is fundamental to synthetic biology. Optogenetics has emerged as a powerful tool for genetic control and Medusa brings optogenetics to the next level. By engineering E. coli to respond to multiple light inputs, creating a logical AND gate, we aim to achieve both spatial and temporal control of gene expression. Photosensory transmembrane proteins as well as photoswitchable protein caging were investigated to further expand the existing library of optogenetic tools. For spatial control at the subcellular level, we explored the use of a novel synthetic RNA organelles to manipulate enzymatic activity. Finally, in an effort to promote synthetic biology, we sought the input of the DIY community and chose to illustrate the power of our system by 3D-printing biomaterials.</div>
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<div id=abstract><b>Contact us</b> </br>bettencourt.igem2017@gmail.com</br>
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      <p id="u1585-2"><span class="H1" id="u1585">MEDUSA</span></p>
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      <p id="u1585-3">&nbsp;</p>
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      <p>Bringing control to the 3rd Dimension</p>
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      <p>&nbsp;</p>
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    <div class="clearfix colelem" id="u2432-5" data-sizePolicy="fixed" data-pintopage="page_fixedCenter"><!-- content -->
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      <p id="u2432-2">We believe that biology is something that should be shared. That’s why we went to talk to the DIY biohacking community to connect to citizen scientists and anyone who just loved biology. With their help, we decided to make a microbial 3D printer, an accessible multifunctional and synthetic biology tool.</p>
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      <p>&nbsp;</p>
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      <p>Biomaterials</p>
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      <p>Three different biomaterials with differing/distinct properties were developed on: Calcium Carbonate, Polysilicate and Ph3B. Each material has unique properties and differing applications, allowing for a broad spectrum of fields that the 3D printer belongs to.</p>
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      <a class="nonblock nontext clip_frame colelem" id="u2531" href="project-2.html" data-sizePolicy="fixed" data-pintopage="page_fluidx"><!-- image --><img class="block" id="u2531_img" src="http://maisonchateauneuf.fr/medusa/images/animacion-picto-opto.gif?crc=242435740" alt="" width="150" height="150"/></a>
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      <p>Light control</p>
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      <p id="u2504-2">Optogenetics allows fully reversible control of gene expression in both time and space. Both photosensory transmembrane proteins as well as photoswitchable protein caging were developed to expand the existing library of optogenetic tools.</p>
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      <p>&nbsp;</p>
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      <a class="nonblock nontext clip_frame colelem" id="u2475" href="project-3.html" data-sizePolicy="fixed" data-pintopage="page_fluidx"><!-- image --><img class="block" id="u2475_img" src="http://maisonchateauneuf.fr/medusa/images/animacion-picto-rna.gif?crc=297139977" alt="" width="150" height="150"/></a>
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      <p>&nbsp;RNA Agglomerations</p>
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      <p>RNA is a light cost nucleotide material in the cell, which has potential to act as a scaffold and transporter. We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.</p>
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Revision as of 20:07, 25 September 2017


Home

MEDUSA

 

Bringing control to the 3rd Dimension

 

We believe that biology is something that should be shared. That’s why we went to talk to the DIY biohacking community to connect to citizen scientists and anyone who just loved biology. With their help, we decided to make a microbial 3D printer, an accessible multifunctional and synthetic biology tool.

 

Biomaterials

Three different biomaterials with differing/distinct properties were developed on: Calcium Carbonate, Polysilicate and Ph3B. Each material has unique properties and differing applications, allowing for a broad spectrum of fields that the 3D printer belongs to.

Light control

Optogenetics allows fully reversible control of gene expression in both time and space. Both photosensory transmembrane proteins as well as photoswitchable protein caging were developed to expand the existing library of optogenetic tools.

 

 RNA Agglomerations

RNA is a light cost nucleotide material in the cell, which has potential to act as a scaffold and transporter. We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.