Difference between revisions of "Team:AshesiGhana/InterLab"

(Prototype team page)
 
 
Line 1: Line 1:
−
{{AshesiGhana}}
+
{{Template:AshesiGhana/NavBar}}
 
<html>
 
<html>
 +
  <head>
 +
  <style>
 +
  #illustration-hero-image {
 +
    height: 100vh;
 +
    width: 70%;
 +
    object-fit: contain;
 +
    margin-top: 120px !important;
 +
}
  
 +
.section-content {
 +
    text-align: justify !important;
 +
    font-size: 2.0rem !important;
 +
    font-family: sans-serif !important;
 +
    margin: 10px 0px;
 +
}
  
−
<div class="column full_size judges-will-not-evaluate">
+
.section-block {
−
<h3>★  ALERT! </h3>
+
    max-width: 1000px;
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
+
    margin: 0px auto 50px auto;
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
    padding: 10px 0px;
−
</div>
+
}
−
<div class="clear"></div>
+
  </style>
 +
  </head>
 +
  <body>
 +
    <div >
  
−
<div class="column full_size">
+
<img id="illustration-hero-image" src=" https://static.igem.org/mediawiki/2017/3/39/Intrr.png"/>
−
<h1>InterLab</h1>
+
<div class="section-block ">
−
<h3>Bronze Medal Criterion #4</h3>
+
<p class="section-content">
−
<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range.
+
−
<br><br>
+
−
For teams participating in the <a href="https://2017.igem.org/Competition/InterLab_Study">InterLab study</a>, all work must be shown on this page.
+
−
 
+
−
</p>
+
−
</div>
+
  
 +
<b>Introduction</b> </br>
 +
        The Fourth International InterLaboratory Measurement Study seeks to establish a GFP measurement protocol which could be used by anyone with a plate reader in their labs anywhere in the world. This measurement is however based on engineering principles. The major question being answered by this activity is “How precise will the measurements be when fluorescence is measured all around the world using different instruments?”
 +
</p>
  
 +
<p class="section-content">
  
 +
<b>Materials and Methods </b> </br>
 +
For this study, the team has been provided with a total of 8 plasmids containing all composite parts required to undertake this activity. In addition to the composite parts a measurement kit to enable the team generate and analyse standard curves necessary for the analysis was also provided. This approach standardizes some of the variables of this experiment.
 +
<ul>
 +
<li>Positive control<br><img id="" src="https://static.igem.org/mediawiki/2017/2/2a/Poscont.png"/></li>
 +
<li>Negative control<br><img id="" src="https://static.igem.org/mediawiki/2017/e/ef/Negcont.png"/></li>
 +
<li>Test Device 1<br><img id="" src="https://static.igem.org/mediawiki/2017/d/df/Tdev1.png"/></li>
 +
<li>Test Device 2<br><img id="" src="https://static.igem.org/mediawiki/2017/3/3e/Tdev2.png"/></li>
 +
<li>Test Device 3<br><img id="" src="https://static.igem.org/mediawiki/2017/b/b1/Tdev3.png"/></li>
 +
<li>Test Device 4<br><img id="" src="https://static.igem.org/mediawiki/2017/2/27/Tdev4.png"/></li>
 +
<li>Test Device 5<br><img id="" src="https://static.igem.org/mediawiki/2017/4/43/Tdev5.png"/></li>
 +
<li>Test Device 6<br><img id="" src="https://static.igem.org/mediawiki/2017/2/26/Tdev6.png"/></li>
 +
</ul>
 +
</p>
 +
<p class="section-content">
  
 +
The transformation protocol was adapted from the iGEM protocol, which can be found on this <a href="http://parts.igem.org/Help:Protocols/Transformation">link</a>. All of the devices were transformed and cultured in LB (Luria Bertani) media containing 25 mg/mL chloramphenicol. </br></p>
 +
<p class="section-content">
  
 +
<b>Results</b> </br>
 +
The results of the Interlab Study were tabulated in the following <a href="https://static.igem.org/mediawiki/2017/b/b8/T--AshesiGhana--interrest.pdf">link</a>.</p>
  
  
 +
<p class="section-content">
 +
<b>Team Experience</b> </br>
 +
Engaging in this inter-lab activity was exciting and insightful. Since this was the first time the team was participating in the IGEM competition we took it as an opportunity to engage in as many activities including this inter-lab to have a complete experience of the competition.
 +
Coming from diverse fields in engineering and computer science, we as a team took this as an opportunity to learn new things in the world of synthetic biology and we can confidently say we learnt a lot working on this inter-lab activity.
 +
</p>
 +
</div>
 +
  </body>
 
</html>
 
</html>
 +
   
 +
{{Template:AshesiGhana/Footer}}

Latest revision as of 01:02, 25 October 2017

Ashesi University
College

Introduction
The Fourth International InterLaboratory Measurement Study seeks to establish a GFP measurement protocol which could be used by anyone with a plate reader in their labs anywhere in the world. This measurement is however based on engineering principles. The major question being answered by this activity is “How precise will the measurements be when fluorescence is measured all around the world using different instruments?”

Materials and Methods
For this study, the team has been provided with a total of 8 plasmids containing all composite parts required to undertake this activity. In addition to the composite parts a measurement kit to enable the team generate and analyse standard curves necessary for the analysis was also provided. This approach standardizes some of the variables of this experiment.

  • Positive control
  • Negative control
  • Test Device 1
  • Test Device 2
  • Test Device 3
  • Test Device 4
  • Test Device 5
  • Test Device 6

The transformation protocol was adapted from the iGEM protocol, which can be found on this link. All of the devices were transformed and cultured in LB (Luria Bertani) media containing 25 mg/mL chloramphenicol.

Results
The results of the Interlab Study were tabulated in the following link.

Team Experience
Engaging in this inter-lab activity was exciting and insightful. Since this was the first time the team was participating in the IGEM competition we took it as an opportunity to engage in as many activities including this inter-lab to have a complete experience of the competition. Coming from diverse fields in engineering and computer science, we as a team took this as an opportunity to learn new things in the world of synthetic biology and we can confidently say we learnt a lot working on this inter-lab activity.