Difference between revisions of "Team:William and Mary/PartsTable"

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<!--website by John Mitchell, iGEM 2016
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Bootstrap template by creative-tim-->
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<h2 style='padding-top: 40px; font-family: "Verlag-Book"; font-size: 50px;'>
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Parts
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−
Parts Table
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<div class="exp">
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</p>
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<p></p>
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</div>
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</div>
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</div>
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−
<div class="col-md-7 col-md-offset-1" style='padding-top: 0px;'>
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<div class="description add-animation-stopped animation-1">
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<p class='large' style="color:#A9A9A9;">
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−
  William and Mary iGEM submitted 118 parts to The Registry this year. They are listed in the table below.
+
<!--UNS pdt#3 DT-->
−
</p>
+
 
−
</div>
+
<a class="exp" href="#url" tabindex="1">UNS pdt#3 DT</a>
−
</div>
+
<div class="exp">
−
</div>
+
<p>
−
</div>
+
<div class="row">
−
</div>
+
  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/8/81/T--William_and_Mary--pdttag.jpeg" width=320px></div>
−
</div>
+
  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333401">BBa_K2333401</a> </br></div>
−
+
</div>
−
<div>
+
</p>
−
<table style='width: 75%; margin-left: auto; margin-right: auto;'>
+
</div>
−
<thead>
+
 
−
  <tr>
+
<!--UNS pdt#3a DT-->
−
<th>Part</th>
+
 
−
<th>Description</th>
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<a class="exp" href="#url" tabindex="1">UNS pdt#3a DT</a>
−
  </tr>
+
<div class="exp">
−
  </thead>
+
<p>
−
  <tbody>
+
<div class="row">
−
  <tr>
+
  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/2/23/T--William_and_Mary--pdttaga.jpeg" width=320px></div>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066001"> K2066001 </a></td>
+
  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3a tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333402">BBa_K2333402</a> </br></div>
−
<td>Red Chromoprotein UNS Gibson Cloning Insert </td>
+
</div>
−
  </tr>
+
</p>
−
  <tr>
+
</div>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066002"> K2066002 </a></td>
+
 
−
<td>Tet Monomer A w/ 8 bp spacer for ICA</td>
+
<!--UNS pdt#3b DT-->
−
  </tr>
+
 
−
  <tr>
+
<a class="exp" href="#url" tabindex="1">UNS pdt#3b DT</a>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066003"> K2066003 </a></td>
+
<div class="exp">
−
<td>Tet Monomer B w/ 8 bp spacer for ICA</td>
+
<p>
−
  </tr>
+
<div class="row">
−
  <tr>
+
  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/9/9b/T--William_and_Mary--pdttagb.jpeg" width=320px></div>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066004"> K2066004 </a></td>
+
  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3b tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333403">BBa_K2333403</a> </br></div>
−
<td>Tet Monomer C w/ 8 bp spacer for ICA</td>
+
</div>
−
  </tr>
+
</p>
−
  <tr>
+
</div>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066005"> K2066005 </a></td>
+
 
−
<td>Tet Monomer A w/ 16 bp spacer for ICA</td>
+
<!--UNS pdt#3c DT-->
−
  </tr>
+
 
−
  <tr>
+
<a class="exp" href="#url" tabindex="1">UNS pdt#3c DT</a>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066006"> K2066006 </a></td>
+
<div class="exp">
−
<td>Tet Monomer B w/ 16 bp spacer for ICA</td>
+
<p>
−
  </tr>
+
<div class="row">
−
  <tr>
+
  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/7/7b/T--William_and_Mary--pdttagc.jpeg" width=320px></div>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066007"> K2066007 </a></td>
+
  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3c tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333404">BBa_K2333404</a> </br></div>
−
<td>Tet Monomer C w/ 16 bp spacer for ICA</td>
+
</div>
−
  </tr>
+
</p>
−
  <tr>
+
</div>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066008"> K2066008 </a></td>
+
 
−
<td>Tet Monomer A w/ 64 bp spacer for ICA</td>
+
<!--UNS pdt#3d DT-->
−
  </tr>
+
 
−
  <tr>
+
<a class="exp" href="#url" tabindex="1">UNS pdt#3d DT</a>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066009"> K2066009 </a></td>
+
<div class="exp">
−
<td>Tet Monomer B w/ 64 bp spacer for ICA</td>
+
<p>
−
  </tr>
+
<div class="row">
−
  <tr>
+
  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/c/c8/T--William_and_Mary--pdttagd.jpeg" width=320px></div>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066010"> K2066010 </a></td>
+
  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3d tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333405">BBa_K2333405</a> </br></div>
−
<td>Tet Monomer C w/ 64 bp spacer for ICA</td>
+
</div>
−
  </tr>
+
</p>
−
  <tr>
+
</div>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066011"> K2066011 </a></td>
+
 
−
<td>Lac Monomer A w/ 16 bp spacer for ICA</td>
+
<!--UNS pdt#3e DT-->
−
  </tr>
+
 
−
  <tr>
+
<a class="exp" href="#url" tabindex="1">UNS pdt#3e DT</a>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066012"> K2066012 </a></td>
+
<div class="exp">
−
<td>Lac Monomer B w/ 16 bp spacer for ICA</td>
+
<p>
−
  </tr>
+
<div class="row">
−
  <tr>
+
  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/1/1f/T--William_and_Mary--pdttage.jpeg" width=320px></div>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066013"> K2066013 </a></td>
+
  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3e tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333406">BBa_K2333406</a> </br></div>
−
<td>Lac Monomer C w/ 16 bp spacer for ICA</td>
+
</div>
−
  </tr>
+
</p>
−
  <tr>
+
</div>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066014"> K2066014 </a></td>
+
 
−
<td>plLac0-1 Ribozyme Characterization Part – GFP only</td>
+
 
−
  </tr>
+
 
−
  <tr>
+
</div>
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066015"> K2066015 </a></td>
+
 
−
<td>plLac0-1 Ribozyme Characterization Part – cI-GFP</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066016"> K2066016 </a></td>
+
−
<td>Constitutive Lac Repressor</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066022"> K2066022 </a></td>
+
−
<td>Constitutive tetR</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066023"> K2066023 </a></td>
+
−
<td>pTet GFP</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066024"> K2066024 </a></td>
+
−
<td>pTac Ribozyme Characterization Part – GFP only</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066025"> K2066025 </a></td>
+
−
<td>pTac Ribozyme Characterization Part – cI-GFP</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066026"> K2066026 </a></td>
+
−
<td>plLac0-1 Ribozyme Characterization Part – GFP only; no RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066027"> K2066027 </a></td>
+
−
<td>plLacO-1 Ribozyme Characterization Part – cI-GFP; no RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066028"> K2066028 </a></td>
+
−
<td>pBAD - RBS - LacI/mCherry fusion - DT on UNS</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066029"> K2066029 </a></td>
+
−
<td>pBAD - RBS - TetR/mCherry fusion - DT on UNS</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066030"> K2066030 </a></td>
+
−
<td>pTAC Ribozyme Characterization Part – GFP only; no RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066031"> K2066031 </a></td>
+
−
<td>pTAC Ribozyme Characterization Part – cI-GFP; no RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066033"> K2066033 </a></td>
+
−
<td>pBad RFP</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066034"> K2066034 </a></td>
+
−
<td>Promoter and RBS characterization construct – mCherry + Spinach</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066035"> K2066035 </a></td>
+
−
<td>plLac0-1 RBS Characterization Part – B0034</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066036"> K2066036 </a></td>
+
−
<td>plLac0-1 RBS Characterization Part – B0031</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066037"> K2066037 </a></td>
+
−
<td>pACT-Tet flanked by UNS 2, 3</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066038"> K2066038 </a></td>
+
−
<td>Sigma54 Enhancer 57S, no Double Terminator, flanked by UNS 2, 3</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066039"> K2066039 </a></td>
+
−
<td>Sigma54 Enhancer 57S with Double Terminator, flanked by UNS 2, 3</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066040"> K2066040 </a></td>
+
−
<td>J23100 + RiboJ Promoter Characterization Part with 2x Broccoli</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066041"> K2066041 </a></td>
+
−
<td>J23100 without RiboJ Promoter Characterization Part</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066042"> K2066042 </a></td>
+
−
<td>J23115 + RiboJ Promoter Characterization Part</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066043"> K2066043 </a></td>
+
−
<td>J23115 without RiboJ Promoter Characterization Part</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066044"> K2066044 </a></td>
+
−
<td>plLac0-1 RBS Characterization Part – B0029</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066045"> K2066045 </a></td>
+
−
<td>plLac0-1 RBS Characterization Part – B0064</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066046"> K2066046 </a></td>
+
−
<td>plLac0-1 RBS Characterization Part – B0030</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066047"> K2066047 </a></td>
+
−
<td>plLac0-1 RBS Characterization Part – B0035</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066048"> K2066048 </a></td>
+
−
<td>plLac0-1 RBS Characterization Part – B0032</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066049"> K2066049 </a></td>
+
−
<td>plLac0-1 RBS Characterization Part – B0033</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066050"> K2066050 </a></td>
+
−
<td>F30 scaffold/2x Broccoli</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066051"> K2066051 </a></td>
+
−
<td>J23107 with RiboJ Promoter Characterization Part</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066052"> K2066052 </a></td>
+
−
<td>J23107 without RiboJ Promoter Characterization Part</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066053"> K2066053 </a></td>
+
−
<td>pTet GFP + 6.1 spacer + tetR</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066054"> K2066054 </a></td>
+
−
<td>T7 Promoter with RiboJ Promoter Characterization Part</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066055"> K2066055 </a></td>
+
−
<td>T7 Promoter without RiboJ Promoter Characterization Part</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066057"> K2066057 </a></td>
+
−
<td>Promoter characterization part – 014 with J23100</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066058"> K2066058 </a></td>
+
−
<td>Promoter characterization part – 014 with J23115</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066059"> K2066059 </a></td>
+
−
<td>Promoter characterization part – 035 with J23100, without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066060"> K2066060 </a></td>
+
−
<td>Promoter characterization part – 035 with J23115, without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066061"> K2066061 </a></td>
+
−
<td>Promoter characterization part – 035 with J23101</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066062"> K2066062 </a></td>
+
−
<td>Promoter characterization part – 035 with J23102</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066063"> K2066063 </a></td>
+
−
<td>Promoter characterization part – 035 with J23103</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066064"> K2066064 </a></td>
+
−
<td>Promoter characterization part – 035 with J23104</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066065"> K2066065 </a></td>
+
−
<td>Promoter characterization part – 035 with J23105</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066066"> K2066066 </a></td>
+
−
<td>Promoter characterization part – 035 with J23106</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066067"> K2066067 </a></td>
+
−
<td>Promoter characterization part – 035 with J23108</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066068"> K2066068 </a></td>
+
−
<td>Promoter characterization part – 035 with J23109</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066069"> K2066069 </a></td>
+
−
<td>Promoter characterization part – 035 with J23110</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066070"> K2066070 </a></td>
+
−
<td>Promoter characterization part – 035 with J23111</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066071"> K2066071 </a></td>
+
−
<td>Promoter characterization part – 035 with J23112</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066072"> K2066072 </a></td>
+
−
<td>Promoter characterization part – 035 with J23113</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066073"> K2066073 </a></td>
+
−
<td>Promoter characterization part – 035 with J23114</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066074"> K2066074 </a></td>
+
−
<td>Promoter characterization part – 035 with J23116</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066075"> K2066075 </a></td>
+
−
<td>Promoter characterization part – 035 with J23117</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066076"> K2066076 </a></td>
+
−
<td>Promoter characterization part – 035 with J23118</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066077"> K2066077 </a></td>
+
−
<td>Promoter characterization part – 035 with J23119</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066078"> K2066078 </a></td>
+
−
<td>Promoter characterization part – 035 with R0040</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066079"> K2066079 </a></td>
+
−
<td>Promoter characterization part – 035 with R0010</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066080"> K2066080 </a></td>
+
−
<td>Promoter characterization part – 035 with J23150</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066081"> K2066081 </a></td>
+
−
<td>Promoter characterization part – 035 with J23151</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066082"> K2066082 </a></td>
+
−
<td>Promoter characterization part – 035 with J23101 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066083"> K2066083 </a></td>
+
−
<td>Promoter characterization part – 035 with J23102 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066084"> K2066084 </a></td>
+
−
<td>Promoter characterization part – 035 with J23103 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066085"> K2066085 </a></td>
+
−
<td>Promoter characterization part – 035 with J23104 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066086"> K2066086 </a></td>
+
−
<td>Promoter characterization part – 035 with J23105 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066087"> K2066087 </a></td>
+
−
<td>Promoter characterization part – 035 with J23106 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066088"> K2066088 </a></td>
+
−
<td>Promoter characterization part – 035 with J23108 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066089"> K2066089 </a></td>
+
−
<td>Promoter characterization part – 035 with J23109 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066090"> K2066090 </a></td>
+
−
<td>Promoter characterization part – 035 with J23110 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066091"> K2066091 </a></td>
+
−
<td>Promoter characterization part – 035 with J23111 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066092"> K2066092 </a></td>
+
−
<td>Promoter characterization part – 035 with J23112 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066093"> K2066093 </a></td>
+
−
<td>Promoter characterization part – 035 with J23113 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066094"> K2066094 </a></td>
+
−
<td>Promoter characterization part – 035 with J23114 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066095"> K2066095 </a></td>
+
−
<td>Promoter characterization part – 035 with J23116 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066096"> K2066096 </a></td>
+
−
<td>Promoter characterization part – 035 with J23117 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066097"> K2066097 </a></td>
+
−
<td>Promoter characterization part – 035 with J23118 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066098"> K2066098 </a></td>
+
−
<td>Promoter characterization part – 035 with J23119 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066099"> K2066099 </a></td>
+
−
<td>Promoter characterization part – 035 with R0040 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066100"> K2066100 </a></td>
+
−
<td>Promoter characterization part – 035 with R0011</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066101"> K2066101 </a></td>
+
−
<td>Promoter characterization part – 035 with R0010 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066102"> K2066102 </a></td>
+
−
<td>Promoter characterization part – 035 with R0011 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066103"> K2066103 </a></td>
+
−
<td>Promoter characterization part – 035 with J23150 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066104"> K2066104 </a></td>
+
−
<td>Promoter characterization part – 035 with J23151 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066105"> K2066105 </a></td>
+
−
<td>Promoter characterization part – 035 with J23107</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066106"> K2066106 </a></td>
+
−
<td>Promoter characterization part – 035 with J23107 without RiboJ</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066107"> K2066107 </a></td>
+
−
<td>Promoter characterization part – 035 with J23100</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066108"> K2066108 </a></td>
+
−
<td>Promoter characterization part – 035 with J23115</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066109"> K2066109 </a></td>
+
−
<td>pTet GFP + 6.1 spacer + tetR different rbs</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066110"> K2066110 </a></td>
+
−
<td>placO1 sfgfp + 6.1 spacer + LacI b0034</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066111"> K2066111 </a></td>
+
−
<td>placO1 sfgfp + 6.1 spacer + LacI b0031</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066112"> K2066112 </a></td>
+
−
<td>UNS NRII + DT</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066113"> K2066113 </a></td>
+
−
<td>UNS 55as</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066114"> K2066114 </a></td>
+
−
<td>UNS 52s + UNS3.1 + functional NRII with B0015 double terminator + UNS3.2</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066115"> K2066115 </a></td>
+
−
<td>UNS 55as sfgfp</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066116"> K2066116 </a></td>
+
−
<td>UNS 52s sfgfp</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066117"> K2066117 </a></td>
+
−
<td>UNS 55as NRII</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066118"> K2066118 </a></td>
+
−
<td>UNS 52S</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066119"> K2066119 </a></td>
+
−
<td>UNS 55as sfgfp NRII</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066120"> K2066120 </a></td>
+
−
<td>UNS 52s sfgfp NRII</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066121"> K2066121 </a></td>
+
−
<td>UNS 55as sfgfp NRII TetR</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066122"> K2066122 </a></td>
+
−
<td>UNS 52s sfgfp NRII TetR</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066123"> K2066123 </a></td>
+
−
<td>UNS 55as  NRII TetR</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066124"> K2066124 </a></td>
+
−
<td>UNS 52s NRII TetR</td>
+
−
  </tr>
+
−
  <tr>
+
−
<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2066550"> K2066550 </a></td>
+
−
<td>85x tetO on biobrick origin</td>
+
−
  </tr>
+
−
  </tbody>
+
−
</table>
+
−
</div>
+
 
</html>
 
</html>
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{{Team:William_and_Mary/JS_SOURCE2}}
 
{{Team:William_and_Mary/JS_SOURCE2}}
 
{{Team:William_and_Mary/FOOTER_SMALL}}
 
{{Team:William_and_Mary/FOOTER_SMALL}}

Revision as of 18:28, 5 July 2017

UNS pdt#3 DT

This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.


iGEM registry: BBa_K2333401

UNS pdt#3a DT

This part is designed to easily facilitate appending the protein degradation tag (pdt)#3a tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.


iGEM registry: BBa_K2333402

UNS pdt#3b DT

This part is designed to easily facilitate appending the protein degradation tag (pdt)#3b tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.


iGEM registry: BBa_K2333403

UNS pdt#3c DT

This part is designed to easily facilitate appending the protein degradation tag (pdt)#3c tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.


iGEM registry: BBa_K2333404

UNS pdt#3d DT

This part is designed to easily facilitate appending the protein degradation tag (pdt)#3d tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.


iGEM registry: BBa_K2333405

UNS pdt#3e DT

This part is designed to easily facilitate appending the protein degradation tag (pdt)#3e tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.


iGEM registry: BBa_K2333406