Difference between revisions of "Team:Heidelberg/Demonstrate"
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Cytochrome engineering</div> | Cytochrome engineering</div> | ||
<div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | <div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | ||
| − | + | We engineered the product specificity of cytochrome P450 1A2 using PREDCEL.</div> | |
</a> | </a> | ||
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Protein Interaction</div> | Protein Interaction</div> | ||
<div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | <div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | ||
| − | + | We used PREDCEL to evolve a split T7 polymerase towards improved auto-reassembly.</div> | |
</a> | </a> | ||
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Optogenetics</div> | Optogenetics</div> | ||
<div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | <div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | ||
| − | + | We used light to control selection stringency during PREDCEL</div> | |
</a> | </a> | ||
</div> | </div> | ||
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Organosilicons</div> | Organosilicons</div> | ||
<div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | <div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | ||
| − | + | We used an engineered cytochrome to catalyze carbon-silicon bond formation in whole-cell extracts.</div> | |
</a> | </a> | ||
</div> | </div> | ||
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Evolution of \(\beta\)-Lactamases</div> | Evolution of \(\beta\)-Lactamases</div> | ||
<div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | <div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | ||
| − | + | We observed a correlation of the measured \(\beta\)-lactamase activities with the protein label scores from AiGEM</div> | |
</a> | </a> | ||
<a href="https://2017.igem.org/Team:Heidelberg/Validation#gusgalResults" class="demonstrate-button" style="margin: 0px auto !important; background-color: #f8991d !important; color: white !important;"> | <a href="https://2017.igem.org/Team:Heidelberg/Validation#gusgalResults" class="demonstrate-button" style="margin: 0px auto !important; background-color: #f8991d !important; color: white !important;"> | ||
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Reprogramming of \(\beta\)-Glucoronidase</div> | Reprogramming of \(\beta\)-Glucoronidase</div> | ||
<div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | <div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | ||
| − | + | </div> | |
</a> | </a> | ||
</div> | </div> | ||
Revision as of 00:29, 2 November 2017
Demonstrate
Our concepts & application
In vivo Evolution
Demonstration of our PREDCEL Toolbox:
Cytochrome engineering
We engineered the product specificity of cytochrome P450 1A2 using PREDCEL.
Protein Interaction
We used PREDCEL to evolve a split T7 polymerase towards improved auto-reassembly.
Optogenetics
We used light to control selection stringency during PREDCEL
Organosilicones
Demonstration of Carbon-Silicon-Bond formation:
Organosilicons
We used an engineered cytochrome to catalyze carbon-silicon bond formation in whole-cell extracts.
In silico Evolution
