Difference between revisions of "Team:Paris Bettencourt/Results"

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<div class="textbody" >
 
<div class="textbody" >
 
<h1>Optogenetic</h1>
 
<h1>Optogenetic</h1>
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<div class=text2><div class=text2left><h4>Membrane photosensor</h4></div>
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<div class=text2><div class=text2left><h4>Membrane photosensor</h4><ul><li></li></li></div>
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       <div class=text2right><h4>Protein photocaging</h4></div></div>
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       <div class=text2right><h4>Optics</h4><ul><li> Results confirm the feasibility of achieving a high targeting resolution at significant length within the gel medium.</li></ul></div></div>
 
<h1>Biomaterial</h1>
 
<h1>Biomaterial</h1>
 
<div class=text2><div class=text2left><h4>Calcium Carbonate</h4><ul><li> Coral acid-rich proteins(CARP1-CARP4) were implemented into the expression vector and transformed into Escherichia coli BL21.</li><li> Right size proteins were expressed and checked for calcium carbonate precipitation ability.</li></ul><h4>Polysilicate</h4><ul><li> Characterized a biobrick from iGEM Pasteur</li></ul></div>
 
<div class=text2><div class=text2left><h4>Calcium Carbonate</h4><ul><li> Coral acid-rich proteins(CARP1-CARP4) were implemented into the expression vector and transformed into Escherichia coli BL21.</li><li> Right size proteins were expressed and checked for calcium carbonate precipitation ability.</li></ul><h4>Polysilicate</h4><ul><li> Characterized a biobrick from iGEM Pasteur</li></ul></div>
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<h1>RNA organelle</h1>
 
<h1>RNA organelle</h1>
 
<div class=text1><ul><li> RNA organelle was shown to decrease background expression of GFP, giving evidence to our model.</li><li> The RNA organelle was long-living, as it was observed to irreversible and stable in culture 20h after induction.</li><ul></div>
 
<div class=text1><ul><li> RNA organelle was shown to decrease background expression of GFP, giving evidence to our model.</li><li> The RNA organelle was long-living, as it was observed to irreversible and stable in culture 20h after induction.</li><ul></div>
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<h1>Logic Gate</h1>
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<h1>Logic Gate & Photo-Caging</h1>
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<div class=text1></div>
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<div class=text1><ul><li> Successful testing in cell-free system with Dronpa.</li><li> Found evidence that Dronpa "sticks" the repressors to DNA.</li></ul></div>
 
<h1>Human Practice</h1>
 
<h1>Human Practice</h1>
 
<div class=text2><div class=text2left></div>
 
<div class=text2><div class=text2left></div>

Revision as of 02:08, 2 November 2017

RESULTS

Optogenetic

Membrane photosensor

Optics

  • Results confirm the feasibility of achieving a high targeting resolution at significant length within the gel medium.

Biomaterial

Calcium Carbonate

  • Coral acid-rich proteins(CARP1-CARP4) were implemented into the expression vector and transformed into Escherichia coli BL21.
  • Right size proteins were expressed and checked for calcium carbonate precipitation ability.

Polysilicate

  • Characterized a biobrick from iGEM Pasteur

PHA

  • Improved the characterization of part BBa_K1149051 using flow cytometry to better quantify P3HB production in E. coli.

RNA organelle

  • RNA organelle was shown to decrease background expression of GFP, giving evidence to our model.
  • The RNA organelle was long-living, as it was observed to irreversible and stable in culture 20h after induction.

    Logic Gate & Photo-Caging

    • Successful testing in cell-free system with Dronpa.
    • Found evidence that Dronpa "sticks" the repressors to DNA.

    Human Practice

    Centre for Research and Interdisciplinarity (CRI)
    Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
    Paris Descartes University
    24, rue du Faubourg Saint Jacques
    75014 Paris, France
    bettencourt.igem2017@gmail.com