Difference between revisions of "Team:UChile OpenBio-CeBiB/Experiments"

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<h1>Experiments</h1>
 
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<p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
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<h2> Chlamydomonas reinhardtii growth </h2>
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Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.
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<h5>What should this page contain?</h5>
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<li> Protocols </li>
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<li> Experiments </li>
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<li> Documentation of the development of your project </li>
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<h5>Inspiration</h5>
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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<p> The strain that will be utilized is cell wall deficient, the used in [1] is the strain cw15-30-derived UVM4 C. The culture medium in which they grow are TAP (solid) and TAPS (liquid). Light stimulation will be required, for which a structure capable of covering the Erlenmeyer flasks or the petri dishes in which C. reinhardtii would be growing while uniformly illuminating with the whole visible light spectrum will be designed and built. In [1] the lamp that is put into use has the following characteristics: Nano Light, 11 Watts, Dennerle, Vinningen, Germany, which provided constant light with the following details: 2500 lux, eq. 72.5 μE/m2∙s1. </p>
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<p> For the construction of calibration curves the medium to be used is TAPS without acetate. </p>
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<p> [1] Chávez, M. N., Schenck, T. L., Hopfner, U., Centeno-Cerdas, C., Somlai-Schweiger, I., Schwarz, C., ... & Nickelsen, J. (2016). Towards autotrophic tissue engineering: photosynthetic gene therapy for regeneration. Biomaterials, 75, 25-36. </p>
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<p><i><u>TAP (Tris-Acetate-Phosphate)</u></i></p>
 
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Revision as of 22:05, 8 August 2017

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UChile_OpenBio-CeBiB

Experiments

Chlamydomonas reinhardtii growth

The strain that will be utilized is cell wall deficient, the used in [1] is the strain cw15-30-derived UVM4 C. The culture medium in which they grow are TAP (solid) and TAPS (liquid). Light stimulation will be required, for which a structure capable of covering the Erlenmeyer flasks or the petri dishes in which C. reinhardtii would be growing while uniformly illuminating with the whole visible light spectrum will be designed and built. In [1] the lamp that is put into use has the following characteristics: Nano Light, 11 Watts, Dennerle, Vinningen, Germany, which provided constant light with the following details: 2500 lux, eq. 72.5 μE/m2∙s1.

For the construction of calibration curves the medium to be used is TAPS without acetate.

[1] Chávez, M. N., Schenck, T. L., Hopfner, U., Centeno-Cerdas, C., Somlai-Schweiger, I., Schwarz, C., ... & Nickelsen, J. (2016). Towards autotrophic tissue engineering: photosynthetic gene therapy for regeneration. Biomaterials, 75, 25-36.

TAP (Tris-Acetate-Phosphate)