Team:UChile OpenBio-CeBiB/Results

Results

Here are the lab results

First amplification of pBC1. There was no growth of chemicompetent transformants [09/05/2017].

pBC1 Amplification. Thermal shock transformation [09/06/2017].

Electrophoresis results [10/13/2017]

Gel visualization of amplification of the gblocks Est2 and Est4

Gel visualization of the sequences of the second order made to IDT

Gel extraction for purification of the gBlock of the second order made to IDT

Selected colonies for miniprep [10/19/17]

LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1-Se 1_2. It grows.

LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1-Se 2_2. It grows.

LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBS1-SEQ. It do not grows.

LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3-Est1_2. It grows.

LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3-Est2. It grows

LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3. It grows

LB and Ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1. It grows

Results of the third restriction test

Superior gel. It is indicated with (N °) the colony from which the plasmid comes. Staircase of 1 kB. Nomenclature: Part-N ° of colony-Plasmid to which the part is bound (Lig plasmid)

Lower gel. It is indicated with (N °) the colony from which the plasmid comes. Staircase of 1 kB. Nomenclature: Part-N ° of colony-Plasmid to which the part is bound (Lig plasmid).