Team:SUSTech Shenzhen/Results

Results

Project

Optical Experiments Resutls

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| | First | Second | |--|------|---------| | 3 | 43 | 4 |

Microfluidic Experiments

Here, we fixed the Caenorhabditis elegans in immobilization chip to observe the Odr10::CoChR::GEM-GECO::mCherry worms under the fluorescence microscope and saw the neuronal activity successfully, which can confirm that the worms can express our target genes. Then, we demonstrated that the insertion did not damage the olfactory receptor neuron pairs of the worms by testing their response to diacetyl and 2-nonanone in Gaussian Plate.

Fig.1 A. The immobilization chip. B. The mCherry in Odr10::CoChR::GEM-GECO::mCherry worms. C. The Gaussian Plate. D. The distribution of theOdr10::CoChR::GEM-GECO::mCherry worms in Gaussian Plate.

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Behavioral Experiments

Here, we confirmed that the Odr10::CoChR::GEM-GECO::mCherry worms could sense the blue light by inducing the Odr10::CoChR::GEM-GECO::mCherry worms to crawl a cycle on NGM plate. Then, in order to study the worms' learning ability we put the worms in alcohol layer on the NGM plate and stimulated them by the blue light at the same time. After 2 hours' training we found that the worms could crawl towards to the alcohol.

Fig.1 A. The device made by mercury lamp and optical fiber to induce the worms to crawl a cycle. B. The worm under the microscope when doing inducing experiment. C. The device made by mercury lamp and microscope to train the worms (More Details for Hardware) . D.Using the alcohol to induce the worms.

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