Team:William and Mary/Parts
Submitted Parts to the iGEM Registry
This year we are proud to submit a series of parts allowing for the modular control of gene expression speed, using the mf-Lon/pdt protein degradation system. These parts will enable users to predictably control the temporal dynamical behavior of their gene of choice, incorporating the speed-control system seamlessly into their circuit with our easy-to-clone parts. We include a number of variations in reporter protein and inducible promoter/respective inducer molecule, so that teams can tailor to the requirements of their unique circuit of interest. Additionally, every composite part submitted is flanked by the Unique Nucleotide Sequences (UNS’s) introduced to iGEM by W&M’s 2016 team to improve ease and reliability of cloning.
UNS PDT Parts
test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test
| K2333401 | Cloning Ready pdt #3A with Double Terminator |
| K2333402 | Cloning Ready pdt #3B with Double Terminator |
| K2333403 | Cloning Ready pdt #3C with Double Terminator |
| K2333404 | Cloning Ready pdt #3D with Double Terminator |
| K2333405 | Cloning Ready pdt #3D with Double Terminator |
| K2333406 | Cloning Ready pdt #3F with Double Terminator |
UNS PDT Parts
test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test
UNS PDT Parts
test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test
UNS PDT Parts
test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test
UNS PDT Parts
test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test
All Submitted Parts
| K2333401 | Cloning Ready pdt #3A with Double Terminator |
| K2333402 | Cloning Ready pdt #3B with Double Terminator |
| K2333403 | Cloning Ready pdt #3C with Double Terminator |
| K2333404 | Cloning Ready pdt #3D with Double Terminator |
| K2333405 | Cloning Ready pdt #3D with Double Terminator |
| K2333406 | Cloning Ready pdt #3F with Double Terminator |
| K2333407 | UNS J23100 sfGFP pdt #3A |
| K2333408 | UNS J23100 sfGFP pdt #3B |
| K2333409 | UNS J23100 sfGFP pdt #3C |
| K2333410 | UNS J23100 sfGFP pdt #3D |
| K2333411 | UNS J23100 sfGFP pdt #3E |
| K2333412 | UNS J23100 sfGFP pdt #3F |
| K2333413 | J23100 mScarlet-I |
| K2333414 | J23100 mScarlet-I pdt #3A |
| K2333415 | J23100 mScarlet-I pdt #3B |
| K2333416 | J23100 mScarlet-I pdt #3C |
| K2333417 | J23100 mScarlet-I pdt #3D |
| K2333418 | J23100 mScarlet-I pdt #3E |
| K2333419 | J23100 mScarlet-I pdt #3F |
| K2333420 | UNS pTet sfGFP |
| K2333421 | UNS pTet sfGFP pdt #3A |
| K2333422 | UNS pTet sfGFP pdt #3B |
| K2333423 | UNS pTet sfGFP pdt #3C |
| K2333424 | UNS pTet sfGFP pdt #3D |
| K2333425 | UNS pTet sfGFP pdt #3E |
| K2333426 | UNS pTet sfGFP pdt #3F |
| K2333427 | UNS pTet mScarlet-I |
| K2333428 | UNS pTet mScarlet-I pdt #3A |
| K2333429 | UNS pTet mScarlet-I pdt #3B |
| K2333430 | UNS pTet mScarlet-I pdt #3C |
| K2333431 | UNS pTet mScarlet-I pdt #3D |
| K2333432 | UNS pTet mScarlet-I pdt #3E |
| K2333433 | UNS pTet mScarlet-I pdt #3F |
| K2333434 | pLac0-1 mf-Lon |
| K2333435 | pBad mf-Lon |
| K2333437 | Copper Sensor pdt #3A |
| K2333438 | Copper Sensor pdt #3B |
| K2333439 | Copper Sensor pdt #3C |
| K2333440 | Copper Sensor pdt #3D |
| K2333441 | Copper Sensor pdt #3E |
| K2333442 | Copper Sensor pdt #3F |
