Team:SUSTech Shenzhen/Results

Results

Project

Optical Experiments Resutls

Under construction

| | First | Second | |--|------|---------| | 3 | 43 | 4 |

Microfluidic Experiments

Here, we fixed the Caenorhabditis elegans in immobilization chip to observe the Odr10::CoChR::GEM-GECO::mCherry worms under the fluorescence microscope and saw the neuronal activity successfully, which can confirm that the worms can express our target genes. We also put the Odr10::CoChR::GEM-GECO::mCherry worms into the chip, after a few minutes the worms would be inactive, then we can "wake up" the worms by the blue light.

Fig.1 A. The immobilization chip. B. The worms in immobilization chip.

Then, we demonstrated that the insertion did not damage the olfactory receptor neuron pairs of the worms by testing their response to diacetyl and 2-nonanone in Gaussian Plate.

Fig.1 A. The Gaussian Plate. B. The worms in Gaussian plate.

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Behavioral Experiments

Here, we confirmed that the Odr10::CoChR::GEM-GECO::mCherry worms could sense the blue light by inducing the Odr10::CoChR::GEM-GECO::mCherry worms to crawl a cycle on NGM plate. The Odr10::CoChR::GEM-GECO::mCherry worms could follow the blue light spot just like the attract of the food.

Fig.1 A. The device for light inducing exoeriment made by mercury lamp and optical fiber. B. The worm under the microscope when doing inducing experiment.

Then, in order to study the worms' learning ability we put the worms in alcohol layer on the NGM plate and stimulated them by the blue light at the same time. After 2 hours' training we found that the worms could crawl towards to the alcohol.

Fig.1 A.The device made by mercury lamp and microscope for training the worms.(More details in Hardware B. Using the alcohol to induce the worms.

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Plasmid Construction Results

According to the design of plasmid construction, we constructed ord-10::CoCHR::GEM-geco::mCherry and str-1::Chrimson::GEM-GECO::GFP fusion genes in backbone pCFJ909 successfully. The fusion gene segments were all be sequenced.

We also amplify B series plasmid in miniMos system for microinjection. We integrated ord-10::CoCHR::GEM-geco::mCherry and str-1::Chrimson::GEM-geco::GFP fusion genes into C.elegans(Caenorhabditis elegans) by microinjection respectively.

ord-10::CoCHR::GEM-geco::mCherry

str-1::Chrimson::GEM-GECO::GFP

Made by from the elegans.Inc in SUSTech_Shenzhen.

Licensed under CC BY 4.0.

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