Team:Paris Bettencourt/Logic Circuit

Introduction & Background

For our main system to form, where production of a biomaterial, only occurs when two specific lights intersect, a logic-gate needed to be developed. To create a NOR-gate at the promoter level, we aimed at creating dually repressed promoters. Most of NOR-gate promoter designs using tandem repressible promoters (1, 2, 3) have unpredictable properties and leaky expression. The main challenge to create a clean design of NOR-gate containing only one transcription starting point is the lack of standard transcriptional elements smaller than repressible promoters. Recent work on transcription elements showed that assembling insulated synthetic operator upstream and downstream of an insulated T7 promoter core allowed for a more diverse control of gene expression and a more specific response time (1).

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France

bettencourt.igem2017@gmail.com