Proof of concept - 3D printer:
by creating a printer, it is a clear and easily quantified way to validate our 3D controls.
It also allowed us to engineer biomaterials that would be compatible and useful in
biotech. We also optimised production by creating local concnetrations of enzymes in
the RNA organelles.
By creating logic circuits with photo sensitive proteins, we control and study cells in 3D.
Optogenetics allows fully reversible control of gene expression in both time and space. Both photosensory transmembrane proteins as well as photoswitchable protein caging were developed to expand the existing library of optogenetic tools.
RNA Agglomerations
RNA is a light cost nucleotide material in the cell, which has potential to act as a scaffold and transporter. We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.