Team:William and Mary/Parts
iGEM registry: BBa_K2333401 
iGEM registry: BBa_K2333401
iGEM registry: BBa_K2333401
iGEM registry: BBa_K2333401
iGEM registry: BBa_K2333401
iGEM registry: BBa_K2333401
UNS pdt#3 DT
UNS pdt#3a DT
UNS pdt#3b DT
UNS pdt#3c DT
UNS pdt#3d DT
UNS pdt#3e DT
This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.
This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.
This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.
This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.
This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.
This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.