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...aldehyde. The aldehyde was then condensed with <i>N</i>-methylcysteine to yield the pyochelin. Due to poor commercial availability, <i>N</i>-methylcysteine ...g of a pale-yellow, foliaceous solid were obtained. This corresponds to a yield of 68 %.</u><br>

...ow that the production inside a peroxisome can greatly increase nootkatone yield. Afterwards we discuss possible ways to overcome the shortage of the cellul plt.ylabel(r&quot;Yield in $\frac{mol}{cell}$&quot;)

<p>We also tried to measure the yield of γ－aminobutyric acid (GABA) by using amino acid analyzer (AAA). Firstl ...supernatant was processed and tested. Fig. 4 shows the result of the GABA yield by <i>E. coli</i> Nissle with the constructed plasmid. However, there was n

.... Ignoring the finite resources of the cell may skew our prediction of the yield of our expression systems. See below for details on the whole cell model we ...l model from [1], and performed in silico experiments, comparing predicted yield and parameter scanning to discover any insights into how to optimise recomb

...mentation process, problems like low efficiency of gene expression and low yield always occur. The formula of culture media and the condition of culturing i ...25 ,the yield of BC the most optimal.Meanwhile, when pH=5.25 and 4.75, the yield of BC are higher than the control group by 11% and 7%.</p>

...m.com/eu/products/escherichia-coli-media/media-for-optimal-cell-growth-and-yield-of-e-coli-cultures.html <div class = "title"><p>Yield: </p></div>

the specific rate of sugar production and the yield of biomass on sugar at each position in the bulb.</p> <br>Y_phm^c = Maximal yield of sugar on photons</p>

acid yield by HPLC after whole cell catalysis. Because there are a large number yield multiple is a new indicator, the result is as follows:

...ub(8|16)|[su]xt(a?h|a?b(16)?)|srs([id][ab])?|swpb?|swi|smi|tst|teq|wfe|wfi|yield)(eq|ne|cs|cc|mi|pl|vs|vc|hi|ls|ge|lt|gt|le|al|hs|lo)?[sptrx]?",end:"\\s"},e ...ng from get group into join let orderby partial select set value var where yield",n=e.IDENT_RE+"(<"+e.IDENT_RE+">)?";return{aliases:["csharp"],keywords:t,il

...em>^[1], which allows us to predict optimal steady-state biomass yield, which is tightly correlated with cell growth rate and is the most likely o ...itially contained 1550 reactions and 1556 metabolites. The optimal biomass yield was found to be \( 224.3248 (\text{g dry weight}\cdot\text{h})^{-1} \). Nex

...iments that would screen the following factors for their effect on protein yield: <br/><ul> ...ual factors and the two-way interactions between the factors that affected yield (see <b>Figure 8<b>). </p>

...<li>Optimized co-culture media can increase the yield of BC by16.7%</li> ...ering,and the yield of BC's is also low. According to our finding that the yield of BC was higher than that of <i>G.xylinus</i> cultured individually when c

...in BC synthesis process. Through these two methods we hope to achieve high yield of BC . Therefore, we designed the following experiments:</p> ...fer molecule of bacteria.the goal is to implement both to realize the high yield of BC.</p>

...<li>Optimized co-culture media can increase the yield of BC by16.7%</li> ...ering,and the yield of BC's is also low. According to our finding that the yield of BC was higher than that of <i>G.xylinus</i> cultured individually when c

...roduction of alcohols, large-scale bacterial fermentation is often used to yield more product. However, when the alcohol level in the growth medium nears th ...creasingly important. However, mining, refining, and recycling of them may yield hazard by-product if not properly managed. Therefore, we have attempted to

<li>Measured the TetR gene concentration. Low yield of 4.3ng/microL obtained. Sample not workable and quite contaminated. Gel e ...el and excised. Yield of DNA and purity measured using the nanodrop. - Low yield of 6.6ng/microL.</p>

<h3> How to Improve the Yield of Bacterial Cellulose(BC)?</h3> ...ution as buffer. After measure the parameters of pH, residue sugar and the yield of BC after 10days of fermentation. We confirm the optimal pH for the ferme

...pective of the growth rate, as long as cells are illuminated, the fumarate yield on biomass is constant. This result also support theoretical predictions, w ...e day is growth coupled. We calculated the fumarate yield during the day, yield

...isomes when growing under glucose-rich conditions, biosynthesis and target yield can be increased by altering peroxisome size and number. A short introducti ...showing both number and size increase, also leading to a higher metabolic yield. However, a Pex31,32 knockout has been proven to attribute to a change in t

<p>where \(Y\) is known as the <i>yield constant</i></p><br> & = \textrm{input} - \textrm{output} - \frac{\textrm{growth}}{\textrm{yield constant}}

...ons into the natural stress-resistant peroxisomes and thereby increase the yield and efficiency of rare biomolecule production <em>in vivo</em>. ...ytosolic enzymes with membrane bound ones to see if there is any change in yield.</p>

catalyst MoBiFe, the yield of acrolein can reach more than 90%. The reaction ...Mo, Bi, Fe, Co, K and O. The yield of acrylic acid can reach 97.5%. The yield

For the HisTrap column, we observe a purification yield equal to 72.1 %. For the Gluthation Agarose Resin we observe a purification yield equal to 32%.

...ns into the natural stress-resistant peroxisomes and thereby increases the yield and efficiency of rare biomolecule production <em>in vivo</em>. ...ytosolic enzymes with membrane bound ones to see if there is any change in yield.</p>

...tion volume between 20-50&nbsp;&mu;L does not significantly reduce the DNA yield. However, elution volumes less than 10&nbsp;&mu;L are not recommended.</sma ...imer-dimers, purification without isopropanol is recommended. However, the yield of the target DNA fragment will be lower.

// If there's more to do, yield // The conditional operator is the lowest precedence operator (except yield,

...cal pesticides. The yield of herbicides decreased 10.87% compared with the yield in April 2016. From January to April 2017, Chinese chemical pesticide produ <th>Yield in April/ton</th>

...etic organelle presents a suitable strategy to increase the efficiency and yield of non-native pathways. A common approach is to build up artificial compart ...ile both alcohol dehydrogenase and p450-BM3 have only minor effects on the yield. </p>

...Part</a>) but also produces Glucose, Mannose and other sugars reducing the yield of production. ...ed to generate a bank of mutant epimerases to find an enzyme with a better yield. In industries, biologists and PI informed us about the difficulties in enz

<li>Measured the TetR gene concentration. Low yield of 4.3ng/microL obtained. Sample not workable and quite contaminated. Gel e ...el and excised. Yield of DNA and purity measured using the nanodrop. - Low yield of 6.6ng/microL.</p>

..., 2015). One study looking at PHA production from food waste estimated the yield of 0.05 g of PHA per g of COD applied (Rhu, Lee, Kim & Choi, 2003). Another ...n be produced per year in Hong Kong depending on COD content and using PHA yield of 0.11 kg of PHA per kg of COD. PHB production from leachate was also cons

The qPCR did not yield an result. In the following, qPCR was not referred to as a option for phage ...inal domain of the T7 RNAP. Even the sequencing of over 10 plaques did not yield any mutations in the essential domain.

<br> In order to determine the yield of the target product, we chose to observe the efficiency of the DHAP yield estimation system in view of the lack of basic ceaS2 enzyme data.

...ch is important with respect to the project as it predicts the theoretical yield of desired product (tagatose) in our system, is visualized through an enzym ...ects the efficiency, while that of the substrate will increase the overall yield</b>.</p>

$\ce{R}$ is first transcribed to yield the mRNA $\ce{M_R}$. The transcript is then translated into the protein $R$ yield the corresponding repressed operators $\ce{R_2O_N}$ and $\ce{R_2O_E}$. This

<p><strong>Cycle number</strong>: Generally, 25–35 cycles yield sufficient product. For genomic amplicons, 30-35 cycles are recommended.</p ...Buffer at a higher temperature (37°C to 50°C) may slightly increase the yield, especially of large (> 10,000 bp) DNA plasmids.</li>

...e cell wall is essential for efficient lysis. Longer incubation time might yield better results.</i></li> ...oom temperature prior to centrifugation will give a modest increase in DNA yield per elution.</li>

<h2>Optimise recombinant Insulin/Winsulin Yield</h2> <h4>Increasing yield would be the most important aspect of improving our product to make large s

...nt energy market due to the absence of polluting emissions and high energy yield. As hydrogen fuel cells generate energy via the redox reaction of hydrogen ...ith the <i>C. reinhardtii</i> FNR, we aim to get closer to the theoretical yield for hydrogen gas production from glucose, which is twelve molecules of hydr

...om the cytosol into the peroxisome and by this increases the productivity, yield and efficiency of the pathway’s end product. We chose nootkatone and viol ...s would be to develop a more efficient system. An easy way to increase the yield of compounds in the supernatant is to manipulate the <a href="https://2017.

...atial organization of enzymes belonging to a certain metabolic pathway the yield of this pathway may be increased. This was for example investigated for the To investigate whether applying metabolic channeling enhances the yield of the indole-3-acetic acid pathway we devised two different approaches nex

SDS 10⁹ seems to have lower yield than NaOH -> maybe more SDS? (10⁸ has no problems...) Terrible yield, repeat again to be sure

SDS 10⁹ seems to have lower yield than NaOH -> maybe more SDS? (10⁸ has no problems...) Terrible yield, repeat again to be sure

<li> 10 nmol ligation because not 100% yield, therefore we need to make more than necessary.</li> ...r at least 20 minutes (can leave overnight at this temperature for greater yield)</li>

As shown in Fig.1, multiple synthesis pathways could easily be imagined to yield the desired compound. ...ed system. This compound is then hydrolysed by addition of aqueous acid to yield the formyl-derivative of the phenolic compound. In the same step the urotro

...<li>Superior broth has more nutrients than LB and therefore has a higher yield</li> ...<li>Note: Using more elution buffer will increase overall yield of DNA but will result in final solution of eluted DNA being less concentra

<p> Both gel extracted samples barely have a yield and the product is the wrong size.</p> ...amplification of the insert (on the Gibson assembly mix) to produce a high yield of product. If this works, we could then ligate the product to the pGEM clo

...ls per milliliter for an overnight culture [3], this would correspond to a yield of $0.432$ ...ed <i>in vitro</i> under equimolar amounts of GFP1-9 and GFP10-SR-GFP11 to yield a time-intensity

alt='The relation between photosynthetic rate and total yield' <th>the instantaneous yield coefficient of product formation due to cell growth</th>

...A lot of EFC relate theses fixes diaphorase onto the electrode for higher yield, and stabilization of the enzymes. </p><Br> ...ng the prototype EMFC we designed, our next goal is to improve electricity yield. This includes improving the battery device with better electrodes, optimiz

...its wider application. Therefore, our project is dedicated to increase the yield of bacterial cellulose ...ay and external fermentation conditions of G.xylinus hoping to improve its yield of BC

...ssful use of dissolved air flotation (DAF) to separate PHB with an overall yield of 86% in lab-scale experiments (van Hee, Elumbaring <i>et al.</i>, 2006). .... The process also has not been used in scaled-up applications yet and PHB yield is comparatively low when compared to solvent extraction. We would also nee

...esired product, but also to optimize growth conditions to maximize product yield within a given timeframe. Take a look at our <a href="https://2017.igem.org

...etic organelle presents a suitable strategy to increase the efficiency and yield of non-native pathways. A common approach is to build up artificial compart ...ile both alcohol dehydrogenase and p450-BM3 have only minor effects on the yield. </p>

...and number of peroxisomes while at the same time lead to higher metabolic yield. For our purpose he recommended working with a <a href="https://2017.igem.o ...ly the intermediates are stored close to the membrane. To achieve a higher yield it was suggested to fuse the relevant enzymes valencene synthase (ValS), P4

...purification concept in which the surface of the column is crucial for the yield instead of the volume compared to conventional purification techniques. ...ely, this could lead to a loss of the target protein and thus decrease the yield. The major challenge concerning the flow-bundle-like column is the irradiat

Cells were fractionated to yield cytoplasmic by the cell lysis solution ,and periplasmic samples by the Argi 3) low yield of recombinant proteins due to weak induction efficiencies (much less than

...>Figure 2.</b> The interaction profile of the input factors, maximised for yield. OD600 at induction consistently correlated with higher yields. The profile ...s within each variable towards the optimum, thus shifting the range of our yield to a higher level.

...uring health and the chemical balance, hydroponic growers can improve crop yield and thus their margins. OxyPonics provides comprehensive, remote, real-time ...this issue - and OxyPonics does just that by boosting crop performance and yield while keeping capital costs to a minimum.

...luding high costs from HVAC systems and low margins due to disease and low yield. We realized that our system needed to be affordable as well as effective i ...ld help the hydroponic industry grow. Since OxyPonics would increase plant yield, we believe there is tangible need for our product. Additionally, OyxPonics

...>An important part in engineering is the simulation of systems in order to yield an insight of the expected behaviours before actual ...for salicylate and estrogen respectively. Concentrations lower than those yield either no or a weak response relative to the maximal

...mentation process, problems like low efficiency of gene expression and low yield always occur. The formula of culture media and the condition of culturing i ...25 ,the yield of BC the most optimal.Meanwhile, when pH=5.25 and 4.75, the yield of BC are higher than the control group by 11% and 7%.</p>

<td>Y_E/g</td> <td><i>E.coli</i>-biomass yield</td> <td>287.54</td> <td>/particle</td> <td>Estimated from experimental dat ...<td>Y_S/g</td> <td><i>S.cerevisiae</i>-biomass yield</td> <td>20.01</td> <td>/particle</td> <td>Estimated from experimental data

<p>Bacterial yield</p> <p>Bacterial yield</p>

...ocess independently, it would be difficult to realistically project price, yield, and environmental impact of the latex manufacturing method; The environmen ...ormation for iGEM Project,"<ul><li> Visit facility and discuss production, yield, and development of PET industry</li><li> Discuss project with manager</ul>

, which already indicates that the search will not yield an unambiguous result. We went to see an expert in the field: the transporter leading to the highest fumarate yield increase in

...ifficult to clone the two gBlocks together, and whenever we succeeded, the yield was too low. We also found a few other mistakes in the design of the sequen ...which should have no colonies due to toxin expression, showed the highest yield. After receiving the sequencing results, we found mutations in some nucleot

...as successful whereas transformation using the cells from our lab didn’t yield good results.</li> <li class="points">Ligation didn’t yield positive result.</li>

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;To increase the yield of the yield of

...uring health and the chemical balance, hydroponic growers can improve crop yield and thus their margins. OxyPonics provides comprehensive, remote, real-time ...this issue - and OxyPonics does just that by boosting crop performance and yield while keeping capital costs to a minimum.

...ol metabolic pathway in organisms and construct a cell factory with a high yield of acrylic acid. acid. However, the yield of the cell factory 1.0 is not high, only about 1mg / L.

...also tested two super-rich media (TB and SOB) in attempt to produce higher yield of bacteria in shorter time. To test the possible growth in minimal media w ...:3%"> To determine which media and temperature is optimal for the best DNA yield cultures in LB, SOB and TB in 37 °C and 42 °C. This experiment was not do

...re, we wanted to test the highest concentration of fructose and see if the yield could be enhanced. So, we used higher concentrations of fructose and we obs ...lass="text-left">Finally, we wanted to investigate if we could enhance the yield of the bioproduction by increasing the IPTG induction, thus increasing the

...to obtain a quantitative analysis of the GST and thus a final purification yield. From this enzymatic activity, a yield can be obtained. Yield = Final total activity / Initial total activity

...system provided that the cell functions normally - the optimal production yield.</p> ...ints that need to be considered at the same time. For the standard optimal yield simulation, a mathematical representation can be formulated in this way:</p

...reas the first plasmid came from a miniprep that is known to give a better yield.</p> ...tending the incubation time after buffer addition to 5 minutes can improve yield.</i></p>

, which already indicates that the search will not yield an unambiguous result. We went to see an expert in the field: the transporter leading to the highest fumarate yield increase in

, which already indicates that the search will not yield an unambiguous result. We went to see an expert in the field: the transporter leading to the highest fumarate yield increase in

, which already indicates that the search will not yield an unambiguous result. We went to see an expert in the field: the transporter leading to the highest fumarate yield increase in

...etic organelle presents a suitable strategy to increase the efficiency and yield of non-native pathways. A common approach is to build up artificial compart ...ile both alcohol dehydrogenase and p450-BM3 have only minor effects on the yield. </p>

...ls because transformations performed with ligation products usually do not yield as many colonies due to the low DNA concentration in the ligation mixture. ...of each concentration if possible, so you can calculate an average colony yield.

...ep up my game. Performed miniprep on G-3 bacteria inoculation culture. DNA yield was only 2.1ng/uL. Fear not, tomorrow will be the day! Continued work on pi <b>Vi Ka:</b> Performed miniprep on G-3 and GFP, with Epoch kit. Achieved yield of 62.7 ng/uL for GF-3, and 144.7 ng/uL for GFP. Prepared digestion for G-3

...ly repress active domains of this enzyme with the intent of increasing the yield of sophorolipid product. By employing a repression technique rather than a

...expression of the biosynthetic pathway, thus maximizing enzyme and pigment yield. </p> <p>Yan, Y., Li, Z., and Koffas, M. A. (2008) High-yield anthocyanin biosynthesis in engineered Escherichia coli. Biotechnology and

...gh (85°C), so using different additives/temperature gradients to optimize yield</li> ...ne + UNS was actually amplified during PCR, and was not visible due to low yield.</li>

...gh genetic engineering could reduce production costs through improving the yield of useful chain lengths.</p>

larger volumes of EB will increase the overall yield of DNA, while using concentration is more important than yield, so go with the 15 µl amount.

<p>Bacterial yield, or bacterial cells per unit substrate</p> ...ed and infected bacteria, multiplied by the inverse value of the bacterial yield (<em>Y</em>), and concentration of inflow and outflow medium:</p>

...the soil and causes root systems to rot, which drastically decreases crop yield in fields nearby. With excessive oil accumulating in the soil, even crops g ...surfactin isoforms produced by different engineers with elevated producing yield, as shown in Fig. S7. Results indicated that overexpression of the three pu

<p><ul><li>Lower recorded yield than other methods</li> ...h secreted and intracellular PHB pellets were dried overnight and relative yield between the two fractions was compared. </p>

...many advantages with the occurrence of inclusion bodies. On one hand they yield a very high protein expression level, on the other, they are easy to isolat <td>Chambon, R., Pradeau, S., Fort, S., Cottaz, S., Armand, S. (2011) High yield production of Rhizobium NodB chitin deacetylase and its use for in vitro sy

<p>Plasmid DNA extractions are performed using Hi Yield^® Plasmid MINI kit according to the <a href="http://www.suedlabo <p>PCR clean-ups and Gel extractions are performed using Hi Yield^® PCR Clean-up/ Gel Extractions Kit according to the <a href="ht

<p> This equation can be solved either analytically or numerically to yield solutions as follows.</p> ...al solution requires intelligent initial and boundary condition choices to yield reasonable results. Computational tools^[6] were used to solve th

...The baculovirus expression system is widely used in biotechnology for high yield recombinant protein expression by exploiting the life cycle of the virus in MAYV genomic RNA was isolated from a Trizol sample. After the integrity and yield of the MAYV RNA was checked, cDNA was generated using Superscript RTII acco

...advantages in our system. First, the protein production is faster, and its yield is higher due to its ability to auto replicate itself. The second one is re ...ral delivery arises as a novel tool due to its high protein expression and yield production. In the last years, it has been reported several virus-derived s

...of CCMB80 buffer needed to add to the resuspended cells to achieve a final yield of OD 600 1.0-1.5. ...://parts.igem.org/Part:BBa_K1321305">K1321305</a>) produced cellulose with yield treatment method was exposed to cell lysate containing phytochelatin-CBD (K

...of CCMB80 buffer needed to add to the resuspended cells to achieve a final yield of OD 600 1.0-1.5. ...://parts.igem.org/Part:BBa_K1321305">K1321305</a>) produced cellulose with yield treatment method was exposed to cell lysate containing phytochelatin-CBD (K

...effect of wild-type ceaS2 enzyme is very weak and the yield of acrylic acid is only 1mg / L. So we ...<br><br> In order to determine the effect of the new route on the yield of acrylic acid before the wet experiment

larger volumes of EB will increase the overall yield of DNA, while using concentration is more important than yield, so go with the 15 µl amount.

...herefore, we decided to change the order of our operon to maximize the PHB yield produced by this pathway.</p>

...g of the biological functions of a given enzyme in vivo or in a controlled yield of intermediate substrates (Bose <i>et al.</i>, 2006).<br> ...for the phytoene synthase which catalyzes the condensation of two GGPP to yield phytoene (Iwata-Reuyl <i>et al.</i>, 2003), (iii) <i>crtI</i> coding for p

...researchers to reach higher yields without the high cost of existing high-yield solutions. ...l gradients in the shaker flask (e.g. temperature and pH) also inhibit the yield.[3] Figure 1 depicts how oxygen depletion corresponds to E. coli entering s

...on lines is the most inefficient one. Focusing on the increasing the final yield would be important to ensure our product produced a net profit from a marke ....org/Team:Calgary/Process">Process</a> would be beneficial to increase PHB yield. Marko also encouraged us to consider the upfront costs of incorporating ou

...more about how <a href="https://2017.igem.org/Team:UCSC/Model">we modeled yield!</a></p>

...vity. Furthermore, according to our expert Prof. Dr. Nedilijko Budisa, the yield of the protein could be influenced by the position the fluorescent amino ac ...not increase the fluorescence intensity. This could be a result of a lower yield or the relatively low fluorescence of CouAA incorporated at position 474 of

...ling reaction as described by Nguyen <i>et&nbsp;al</i>. (2011). Due to low yield compared to Nguyen <i>et&nbsp;al</i>. and 1.05 mmol in the second batch. This equals to the half of the yield of Nguyen <i><i>et&nbsp;al</i></i>. (2011) with 900 mg and 1.89 mmol.

...e <strong>predicted periplasmic expression</strong> to produce the highest yield.</span></p> ...pan style="font-size: 16.0pt; font-family: Quicksand;">We interrogated the yield of folded protein predicted by each model, which allowed us to gain insight

ranging between 0.2 - 2.0µg/µl yield comparable numbers of fluorescent cells, with similar intensity of GFP fluo ...he antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of

...ntial fluorescence production. Device 3 containing promoter J23117 did not yield any fluorescence production. Device 2 containing promoter J23106 was observ

<h4> In order to maximise the yield of our insulin, while also reducing the post-expression methods currently u ...itial test to determine protein folding and accurately quantify expression yield, we will be using an ELISA. This test uses antibodies that bind to the fold

...g proteins from roots to the aerial part of the plant due to its speed and yield. It has not escape to our attention the possible reversibility of viral inf ...s strategy into one of the best strategies for high protein expression and yield production. Thus, we developed our proof of concept for this approach using

Yield: 15 ng/µl Yield: 1 µg/µl

<p>TUST-China iGEM 2017 has been focusing on how to increase the yield of bacterial cellulose and develop its new applications.</p> ...f BC as a wound dressing. Through these work we understood that raising BC yield is an urgent problem to be solved and BC has a lot of good performance that

...many advantages with the occurrence of inclusion bodies. On one hand they yield a very high protein expression level, on the other, they are easy to isolat <td>Chambon, R., Pradeau, S., Fort, S., Cottaz, S., Armand, S. (2011) High yield production of Rhizobium NodB chitin deacetylase and its use for in vitro sy

<li>A significant profit, represented in great savings and greater yield, can be generated through the use of biocontrol; leaving on the side the bi ...umers for chemical-free, sustainably produced food products that ensures a yield improvement on less cultivable land space. Bacterium based biological contr

throw new Error("Comparison should only yield an integer within the interval [-1,1]"); ...mn\" style=\"align-items: center; flex-grow: 1; max-width: 140px;\">\n {{ yield }}\n</div>"

...tially, to identify other interesting targets. Unfortunately, this did not yield many new compounds besides the ones we had already picked up before, except ...p' during the night, metabolically speaking [12]. So although the fumarate yield is not predicted to change, due to drastically decreased overall flux throu

...ndustry will substantiate that people will never conquer the nature . The yield and morbidity of wheat are sheer dependent on the weather . Once the wheat ...o literature review, we know that FHB is responsible for significant grain yield reduction world-wide. In China, wheat scabs affected areas are about 7.5 mi

Repeating elution once will increase DNA yield by 10 ~ 15 %, though DNA will be diluted. ...the DNA using less than suggested volume (50 µl). It will lower the final yield.

...<li>A significant profit, represented in great savings and greater yield can be generated through the use of biocontrol; leaving on the side the bio ...umers for chemical-free, sustainably produced food products that ensures a yield improvement on less cultivable land space. Bacterium based biological contr

...(CouAA) ethylglycine is relatively small, has a high fluorescence quantum yield and relatively large Stoke's shift. It is also solvent polar and pH-sensiti ...e biological functions of a given enzyme <i>in vivo</i> or in a controlled yield of intermediate substrates. Investigations of biological functions of prote

ranging between 0.2 - 2.0µg/µl yield comparable numbers of fluorescent cells, with similar intensity of GFP fluo ...he antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of

...9 and 10 and combine the eluates. A second elution step will increase the yield by approximately 10–15% </li> ...of each concentration if possible, so you can calculate an average colony yield.</li></ul>

...ubtracted from the image taken of bacteria fluorescence under stimulus, to yield an image of bacteria fluorescence without auto-fluorescence.</p> <p>This did not yield accurate results, as it was difficult to align the two images with perfect

...of quick acting fertilizer, urea, in great amount, which can increase rice yield. When the massive loss of nutrient occurs in soil, a great amount of silico ...llution, but also to promote the growth of plants, thereby increasing crop yield. Won in one fell swoop!

...ffects of different circuit designs (one-cycle vs two-cycle system) on the yield tagatose.</p></td> ...design, to understand how its different components may influence tagatose yield.</p></td>

...RSM. During this process, we collected a series of data which based on the yield as main target and the pH residue as co-factor, the data is present below: <li>Factors affecting the yield and properties of bacterial cellulose </li>

ranging between 0.2 - 2.0µg/µl yield comparable numbers of fluorescent cells, with similar intensity of GFP fluo ...he antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of

...chemically synthesized, which employs complex steps yet obtains low yield, bringing the ratio of the four enzymes with a higher yield of N-acetylserotonin (NAS), and to choose

...protein network, where other proteins comprise different fluorophores, may yield significant information on interactions and localization. ...protein network, where other proteins comprise different fluorophores, may yield significant information on interactions and localization.

...es the combination which produced the best results and gave us the highest yield.

...triction site flanks the Gm gene. Therefore, unsuccessful digestion would yield a product around 800 bp. 1. Ladder <span class="underline">M</span>, 2. C18 ...ct OmpR responsive promoter-YFP into a low copy number plasmid (pSB4A5) to yield higher levels of fluorescence. pSB4A5 originally contained a RFP insert, ma

...or assembly. Plasmid pSB1C3 was cut with Spe1 and EcoR1 before assembly to yield the full prefix and suffix, not the truncated form that can be found in the

...e chosen pathogens had to be lysed and their target sequences amplified to yield an amount of target RNA that could be detectable by Cas13a. Finally, we nee ...dissociation constant of K_d = 390 nM. It increases the quantum yield of DFHBI from 0.0007 when free to 0.72 when bound to the aptamer. Spinach h

...probabilities (orange) remain below 1 in 10 million per site. B) crRNA 1-3 yield off-target probabilities below 1 in 10 million for all possible sequences o

...probabilities (orange) remain below 1 in 10 million per site. B) crRNA 1-3 yield off-target probabilities below 1 in 10 million for all possible sequences o

...ecrease the overall yield of eluted DNA. <br/> To increase the overall DNA yield by 20-30% an additional elution step (optional) with Elution Buffer (0.15 m

...chemically synthesized, which employs complex steps yet obtains low yield, bringing the ratio of the four enzymes with a higher yield of N-acetylserotonin (NAS), and to choose

connected to yield a model based representation of the system. The representation then generat the subsequent transcriptional activation by salicylate to yield GFP1-9. In (d), GFP10-ER-GFP11 is translated and activates by the addition

...in methanol at room temperature during this time, which might have reduced yield significantly. ...n from <i>S. coelicolor</i> M1146 / Clo BG1. HPLC analysis showed very low yield which made further purification not feasible. </li>

...probabilities (orange) remain below 1 in 10 million per site. B) crRNA 1-3 yield off-target probabilities below 1 in 10 million for all possible sequences o

...cepted as substrate by the amidase CloL or CouL even tough with a very low yield. <br> The SDS-PAGE does not indicate a high yield of recombinant protein and shows a yet unexplained signal around 70kDa. The

<p>Next, we thought that adding trigger at different time points may yield faster output. yield. We performed 7 different reactions at different time points:

...o maximize the interaction with the substrate, which allowed an incredible yield of 70% at 45°C with a mutated Dpe [6].</p> ...fructose into psicose could be doubled with this technique, for instance a yield of 31% at 65°C [7].</p>

...ed varying levels of expression in the RBS library, and increased geraniol yield from some growth substrates. Additionally, the team initiated an Archaeal I

<li>Measurement about the yield of oil in the algae</li>

...ke a look at what it looks like. Try things out and see what results they yield.</p>

...glycerol (-80 &#2103) after electroporation incubation can be expected to yield similar results to the cells that were plated directly after the incubation

...MegaHIT, which allowed them to stitch together sequences to get a fragment yield of 1,000 base pairs. </br>

...cannot name the exact reason for this result, be it a higher fluorescence yield of CFP, a higher expression or faster folding time of CFP. This reasons cou

...erans and lepidopterans, and proteolytically activated by gut proteases to yield the active toxins of ~65-kDa. Subsequently, the activated toxins bind speci

increasing the incubation time to up to 4 min can increase the yield of

With a total of 2320 km travelled our trips yield a total of 844.5 kg of CO₂.

...glycerol (-80&deg; C) after electroporation incubation can be expected to yield similar results to the cells that were plated directly after the incubation

...ironmental Protection Agency</i> 1977). <b>Our goal is to increase biofilm yield in a common laboratory strain <i>E. coli</i> K-12 by constitutively overexp

...stry, towards making a product that would essentially help to increase the yield and efficiency of for example cheese. In order to create a relevant product

...he main objective is to make the process efficient, clean, and with a high yield. The obtained bioplastic has multiple applications; for example, it can be

...ver time, comparing simultaneous induction to pre-induction of protease to yield qualitatively different results. </div>

...f bacterial cellulose 100 years ago, its application is limited due to low yield and lack of understanding to its physical property. In recent decades, as s

...iate excitation/emission peaks, a large extinction coefficient and quantum yield, and comparable photostability to eGFP. What we have done is to make full u

...ltration, it required large volumes of culture and small quantities of the yield product.

...AB operon</a> submitted in 2013. The gene order was changed to improve PHB yield (Hiroe <i>et al.</i>, 2012), codons were optimized for expression in <i>E.

...o precisely model biological information is indeed admirable, although the yield was negligible.

...rs, pH, temperature) to modify the equilibrium constant, and a large, pure yield of xylulose could theoretically be isolated. However, this is a lengthy pro

...be able to start investigating methods that could be used to increase the yield, and decrease the post-expression processing steps currently required to pr

...and mention the effects our project has on a cow’s meat, taste, or milk yield since companies might be more interested in those aspects. His communicatio

...Violacein gene set with two different inducible promoters, we are able to yield up to four different color outputs. These outputs are measured by an open-s

...has to be integrated into the yeasts chromosomes. This one as much smaller yield.</li>

...o test with, even in the best case. Since DcuC-ec showed a higher fumarate yield increase (Yang paper) and has as a major function ‘efflux’, I suggest t

...platform and screened for ceaS2 mutants that own better acrylic acid yield by HPLC(High Performance Liquid Chromatography)

relatively small, has a high fluorescence quantum yield and relatively large

...y, we are developing a product that would essentially help to increase the yield and efficiency of dairy production, for example, cheese production. In orde

enhances the yield and solubility of Factor VIII proteins. The proteins will be expressed in

...kiwifruit growers cite winter temperatures as the greatest threat to crop yield. Source: Cradock-Henry, N. (2017).

...he model to score potential crRNA sequences based upon their likelihood to yield false-positive results (<b>Q5</b>). Finally, we propose how to experimental

With a total of 2320 km travelled our trips yield a total of 844.5 kg of CO₂.

...ethanol synthesis do not quite meet yeast-standards in terms of conversion yield, bacteria-based mechanisms have greater versatility in cultivation environm

...e and more complex substrates efficiently. For this reason, a high product yield is always sought after.

...chosen for its <i>recA</i> and <i>endA</i> mutations that allow for high-yield minipreps.</p>

...also attempted to purify TLP using a His-tag miniprep kit, but it did not yield any significant results. Now that the semester has started, we anticipate t

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...He, D. (2004). Effects of supplementary ultraviolet-B irradiance on maize yield and qualities: a field experiment. Photochemistry and Photobiology, 80(1),

...rted to useful products in a renewable, environmentally-friendly, and high-yield manner via <i>E. coli</i>. </li>

...nm excitation - emission peaks, a large extinction coefficient and quantum yield, and photostability comparable to that of eGFP. Surface display of recombin

...ntation process, the specific engineering bacteria must be easily grow and yield bio-products efficiently during the long cultivations without any risk of m

...d decreased rates of photosynthesis. The study concluded that overall corn yield decreased with increases in UV-B radiation (5). A second study by Rousseaux

...ate excitation/ emission peaks, a large extinction coefficient and quantum yield, and comparable photostability to eGFP. What we have done is to make full u

...ioned in search results into this tool, modify and improve the pathway, to yield new pathways with aimed functions. Other integrated functional modules, inc

...the protein of interest through optogenetic tools. This is to increase the yield by triggering the expression of the proteins at the best time for the bacte

...Violacein gene set with two different inducible promoters, we are able to yield up to four different color outputs. These outputs are measured by an open-s

...tion to the largely qualitative tests on the market. Because the test will yield results in minutes, it can compete with already marketed tests. Our testing

...previously obtained, loading quality of EchA had little effect on glycerol yield. We constructed a new plasmid which only had the first two enzymes to impro

...n. In this way, several iterations of SELEX and our GA can be conducted to yield high specificity aptamers for any target ligand. </p>

...low GFP. They were also surprised that their minipreps had such a high DNA yield; they were able to check this with the nano drop after completing their min

...some form of selection pressure is upheld, the D.I.V.E.R.T. method should yield improved mutants.</p>

...to modularly change the speed of mScarlet-I, a fast folding, high quantum yield red fluorescent protein. We also created a set of easy-cloning parts which

...ou can also try other ratios (1:1, 2:1, and up to 7:1) if the 3:1 fails to yield good results.

...n [3], it was hoped that the use of this strain would greatly increase the yield of the target dsRNA.

...sed that these factors may be involved in multiple processes and therefore yield difficulties when it comes to creating applicable treatments.<br/><br/>

We used the Promega S30 T7 High-Yield Protein Expression System as our cell free system. After the <i>in vivo</i>

T7-RNA-polymerase amplification system is cloning to yield higher “ON/OFF”ratio.</td>

...of experimental groups of GABA producing circuits and to achieve high GABA yield.</p>

...ysis, this is the table we designed when the PH at the favorable range and yield intend to close to the optimal value:</p>

...test various RBS devices with the intention of finding which combinations yield more stable gene expression and how this might translate to various labs wo

...idth: 100%;"><b>Purpose:</b> The purpose of modeling is to predict product yield, determine feasibility of our project, and potential ways to increase produ

Measuring yield of cellulose <br>

...s process could be optimised to produce even more reproducible results and yield a more robust identification process. Distinct 0 hour expression levels mig

...ethanol synthesis do not quite meet yeast-standards in terms of conversion yield, bacteria-based mechanisms have greater versatility in cultivation environm

<ul><li>25-35 cycles yield sufficient products generally</li>

...side hold the point that transgenic technology can <strong>increase grain yield</strong>, can <strong>alleviate the current difficulties,</strong> can bene

...side hold the point that transgenic technology can <strong>increase grain yield</strong>, can <strong>alleviate the current difficulties, </strong>and can

...hese is 188 kg/km/car (kilde). With a total of 2320 km travelled our trips yield a total of 844,5 kg of CO2.

...scent protein thus far (George Abraham et al., 2015). With a high emission yield of 0.76, NowGFP has several other qualities which makes it the best donor p

...do. The last thing that could be interesting to do would be to enhance the yield of genomic DNA extraction, thus exploring other lysis buffers or changing p

...eluted in a larger volume than usual. We noticed that Ars1.1 had lower DNA yield compared to PcArsR even though they have the same vector backbone. We are n

...can increase the detectability, the stability, bioactivity, and expression yield of the fusion protein (Chen <i>et al.</i>., 2013). Additionally, a direct f

...e and more complex substrates efficiently. For this reason, a high product yield is always sought after.

...e and more complex substrates efficiently. For this reason, a high product yield is always sought after.

<h3>Estimate and optimize yield.</h3>

...pected to reduce the impact of ambient factors on the data of interest and yield fast and reliable data due to the device’s small scale.

...on cubic meters of waste per day. The chemical oxygen demand (COD) and PHB yield were assumed to be 1000 mg/L and 0.11 kg of PHB per kg of COD, respectively

<li> Can compare model predictions (maximum theoretical yield) to experimental data and potentially troubleshoot the system.</li>

...e <i>E. coli</i> arabinose inducible promoter. Though transformations did yield some colonies when trying to make this part, none were red in colour. This

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...<p style="text-align: center;"><span class="quote">Predict and optimize yield</span></p>

...hoice of tet operon. Experimental validation of this system will certainly yield exciting results, which will further shape this model. Also, the pho operon

...class="PP">However, due to the black shank caused by Phytophtora, tobacco yield reduces a lot every year. In China, Hunan province, there's nearly 2.5 mill

...k membrane, increasing the incubation time to up to 4 min can increase the yield of purified DNA.</li>

...so that you can see, based on the growth curves, which concentrations will yield the best results. In one example of the growth curve tests, we used concent

...marketing advantage. At the same time, he was sceptical as our theoretical yield would be only around 31% and proposed the production of more oxidized platf

...et al., “Engineering salt-tolerant Brassica plants: characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium ac

...ng standard derivations of Hill’s equation for transcription factors can yield the probability of activating and inhibiting complexes respectively. Seen b

Weeds and pests are most important damages to the crop yield in the world. Traditional ways like using herbicides and pesticides will ea

...ification column prototype design to reduce dead space, increase potential yield and enhance the scalability. Finally, he recommended focusing on the valida

...lle Aramburu, the incorporation of two noncanonical amino acids lowers the yield. He recommended us to use only one non-canonical amino acid and one cystein

...a combination between positive selection and deep sequencing was enough to yield functional and specific synthetases (Zhang <i>et al.</i>, 2017). During her

of toxic water on the yield (vegetable-fruits)<o:p></o:p></span></p>

...s/suppression of expression of certain genes. The use of transposons would yield bacteria with various phenotypes, which could be used in the <i>Key. coli</

...ead phenomenon called metabolic channelling synthetically and increase the yield in biochemical... <br> <a href="https://2017.igem.org/wiki/index.php?title=

...ural phenomenon called metabolic channelling synthetically to increase the yield of biochemical reactions. Metabolic channelling occurs when the enzymes res

<li>Reduced fruit yield per tree</li>

...ate the ability of DoE to predict concentrations of CFPS supplements which yield optimal protein synthesis activity.</p>

<li><em>This should yield >2 mm colonies that can be used for selection.</em></li>

...of taking samples at specific time points may be more laborious, but will yield a more accurate result of overall culture behaviour.</p>

...em and still have some security margin in case the genetic design does not yield the exact expression levels that we would expect from it. To achieve these

<p> After improving the yield of our cell free protein synthesis system, we

yield for Top10 cells. </p>

Theoretically, this would be followed by anyone and yield comparably robust units of fluorescence, facilitating more meaningful compa

...ain and plates were left in the incubator at 37C for overnight and did not yield expected results.

...is added directly to the TliA gene, which hopefully leads to a good export yield and a high extracellular activity.