Difference between revisions of "Team:AshesiGhana/Collaborations"

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<p>For the 2017 IGEM, Team AshesiGhana is working on a bio-mining project. The objective of our project is to develop an organism capable of liberating gold from the refractory ore. We aim to provide not only an alternative, non-toxic approach for small scale mining, but also deliver an easy and fast bio-detection and quantification method of ore in the mining industry of our country. The availability of such a biosensor will allow routine monitoring of the ore before a mining endeavor is undertaken thus preventing the destruction of the environment. The project will engineer the typical environmental organism Acidithiobacillus ferroxidans with a FRET probe.</p>
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<p>We collaborated with the DTU-Biobuilders team by providing them with the contact information of a prominent snake bites researcher in our country, called Dr. Opoku Manu, since their project is about snake bites. </p>
  
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<p>This new organism will primarily be capable of sensing and quantifying the amount of gold in the ore. This will be accomplished by using a two-part probe, a donor part which is made up of gold binding protein (golB) attached to a green fluorescent protein (nowGFP). The second part of the acceptor is also made up of a binding protein and a red fluorescent protein (mRuby2). In the presence of a high amount of free gold, the two parts would be in close proximity and energy transfer can take place and the red protein would be excited giving off a fluorescent signal. Using calibration experiments, we can relate the amount of fluorescence to the amount of gold present,liberated by the organism from the ore.</p>
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<p>Prior to providing the DTU-Biobuilders team with the contact information of this snake bites researcher, we spoke to him on the behalf of the team and explained to him the primary objective of their project and he was delighted to help.</p>
 
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<p>As Acidithiobacillus ferroxidans is a difficult organism to grow in large quantities, we will engineer a strain of E coli to produce two of the main oxidizing enzymes for iron and sulphite which will liberate the gold from the ore. The organism will also be engineered with protective enzymes against the low pH, which is one of the bi-products of the gold liberation reaction, and metal toxicity. The same FRET biosensor part can be added to the E coli strain for the quantification of gold. This new organism can easily be grown in large batches and so can be used to extract gold from refractory ore without the need of any toxic treatment thus providing a safe alternative for small scale mining. </p>
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<p>In return, the DTU-Biobuilders team provided us with a lot of invaluable advice and guidance in our preparations for this year’s IGEM since they had already been to the Jamboree a couple of times and this was our first time.
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<p>We collaborated with the DTU-Biobuilders team by providing them with the contact information of a prominent snake bites researcher in our country, called Dr. Opoku Manu, since their project is about snake bites. </p>
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<p>Prior to providing the DTU-Biobuilders team with the contact information of this snake bites researcher, we spoke to him on the behalf of the team and explained to him the primary objective of their project and he was delighted to help.</p>
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<p>In return, the DTU-Biobuilders team provided us with a lot of invaluable advice and guidance in our preparations for this year’s IGEM since they had already been to the Jamboree a couple of times and this was our first time.
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We responded to their collaboration request on the IGEM Team Collaborations page by participating in a survey about the state of the quality of air in our country. By participating in this survey, we contributed to a body of information which was vital to the success of their research.
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Revision as of 18:01, 29 October 2017

Ashesi University
College

Collaborations

We collaborated with the DTU-Biobuilders team by providing them with the contact information of a prominent snake bites researcher in our country, called Dr. Opoku Manu, since their project is about snake bites.

Prior to providing the DTU-Biobuilders team with the contact information of this snake bites researcher, we spoke to him on the behalf of the team and explained to him the primary objective of their project and he was delighted to help.

In return, the DTU-Biobuilders team provided us with a lot of invaluable advice and guidance in our preparations for this year’s IGEM since they had already been to the Jamboree a couple of times and this was our first time.

Collaborations

We collaborated with the DTU-Biobuilders team by providing them with the contact information of a prominent snake bites researcher in our country, called Dr. Opoku Manu, since their project is about snake bites.

Prior to providing the DTU-Biobuilders team with the contact information of this snake bites researcher, we spoke to him on the behalf of the team and explained to him the primary objective of their project and he was delighted to help.

In return, the DTU-Biobuilders team provided us with a lot of invaluable advice and guidance in our preparations for this year’s IGEM since they had already been to the Jamboree a couple of times and this was our first time.

Institut Pasteur

We responded to their collaboration request on the IGEM Team Collaborations page by participating in a survey about the state of the quality of air in our country. By participating in this survey, we contributed to a body of information which was vital to the success of their research.