Difference between revisions of "Team:AshesiGhana/Parts"

Line 1: Line 1:
−
{{AshesiGhana}}
+
{{Template:AshesiGhana/NavBar}}
−
<html>
+
  
 +
<html lang="en-GB">
 +
<head>
 +
    <link href='//fonts.googleapis.com/css?family=Raleway%7CMerriweather%7CPT+Mono' rel='stylesheet' type='text/css'>
  
−
<link rel="stylesheet" href="{{Team:AshesiGhana/CSS}}">
+
    <style>
 +
a,nav a {
 +
text-decoration:none
 +
}
 +
.comments div,.meta,code,pre {
 +
font-size:.9em
 +
}
 +
.clearfix:after,.footer-container footer>p {
 +
clear:both
 +
}
 +
img {
 +
vertical-align:middle
 +
}
 +
fieldset {
 +
border:0;
 +
margin:0;
 +
padding:0
 +
}
 +
textarea {
 +
resize:vertical
 +
}
 +
::-moz-selection {
 +
background:#C8E6FD;
 +
text-shadow:none
 +
}
 +
::selection {
 +
background:#C8E6FD;
 +
text-shadow:none
 +
}
 +
body {
 +
background:#fff;
 +
font:1em/2 Merriweather,Georgia,Times,serif;
 +
color:#555
 +
}
 +
a {
 +
color:#494B4B;
 +
border-bottom:1px solid #02BCDC;
 +
-o-transition:.25s;
 +
-ms-transition:.25s;
 +
-moz-transition:.25s;
 +
-webkit-transition:.25s;
 +
transition:.25s
 +
}
 +
a:hover {
 +
color:#02BCDC
 +
}
 +
h1,h2,h3,h4,h5,h6 {
 +
font-weight:400;
 +
font-family:Raleway,Arial,sans-serif;
 +
line-height:1.5em;
 +
color:#666
 +
}
 +
.header-container {
 +
margin:1em 1em 0
 +
}
 +
.title a {
 +
width:181px;
 +
height:51px;
 +
border:0;
 +
text-indent:-9999px;
 +
display:block;
 +
background-image:url(../img/site-name.png);
 +
background-image:-webkit-image-set(url(../img/site-name.png) 1x,url(../img/site-name-2x.png) 2x);
 +
background-position:left center;
 +
background-repeat:no-repeat
 +
}
 +
nav a {
 +
font-family:Raleway,Arial,Sans-Serif;
 +
color:#333;
 +
border:0;
 +
display:inline-block
 +
}
 +
nav ul {
 +
list-style-type:none;
 +
padding-left:0;
 +
margin-left:-1em
 +
}
 +
nav li {
 +
display:inline;
 +
margin-left:1em
 +
}
 +
.main-container {
 +
margin:0 1em 2em
 +
}
 +
code,pre {
 +
font-family:"PT Mono","Courier New",Courier,monospace;
 +
color:#D50085
 +
}
 +
pre {
 +
line-height:1.5em;
 +
margin:1.1em 0
 +
}
 +
article:not(:last-child) {
 +
margin-bottom:2em
 +
}
 +
.meta {
 +
background:#EDF0F3;
 +
padding:.5em 1em
 +
}
 +
.meta h4,.meta p {
 +
display:table-cell;
 +
line-height:2em;
 +
margin:0
 +
}
 +
.meta h4 {
 +
float:left;
 +
margin-right:.5em
 +
}
 +
.comments div {
 +
background:#E9F5F8;
 +
padding:.5em 1em;
 +
margin:1em 0;
 +
word-wrap:break-word
 +
}
 +
.comments div:nth-child(odd) {
 +
background:url(../img/comment-left-bg.png)bottom left no-repeat #E2F2FE;
 +
padding-left:2em
 +
}
 +
.comments div:nth-child(even) {
 +
background:url(../img/comment-right-bg.png)bottom right no-repeat #E2F2FE;
 +
padding-right:2em
 +
}
 +
.comments h4,.comments p {
 +
margin:.5em 0
 +
}
 +
.comments p {
 +
line-height:1.5em
 +
}
 +
.comments h4 {
 +
line-height:1em;
 +
margin-bottom:1em
 +
}
 +
.footer-container {
 +
margin:1em
 +
}
 +
.footer-container footer {
 +
padding-top:1em;
 +
border-top:1px dashed #70828F
 +
}
 +
.footer-container ul {
 +
list-style-type:none;
 +
padding-left:0
 +
}
 +
.footer-container li {
 +
display:inline
 +
}
 +
.footer-container a {
 +
display:inline-block;
 +
background:#366485;
 +
color:#eee;
 +
border:0;
 +
padding:.3em .5em;
 +
margin-bottom:.3em;
 +
line-height:1.2em
 +
}
 +
.footer-container a:hover {
 +
background:#4089BF
 +
}
 +
@media only screen and (min-width:620px) {
 +
.header-container {
 +
  margin:2em 2em 0
 +
}
 +
.header-container .title {
 +
  float:left
 +
}
 +
nav {
 +
  float:right;
 +
  line-height:3.6em
 +
}
 +
.main-container {
 +
  margin:0 2em 2em
 +
}
 +
.meta .category,.meta .date {
 +
  width:25%
 +
}
 +
.meta .tags {
 +
  width:50%
 +
}
 +
.meta div {
 +
  float:left
 +
}
 +
.meta h4,.meta p {
 +
  float:none;
 +
  display:block
 +
}
 +
.footer-container {
 +
  margin:1em 2em
 +
}
 +
.footer-container li {
 +
  display:block
 +
}
 +
.footer-container .about {
 +
  width:70%;
 +
  float:left
 +
}
 +
.footer-container .about h4,.footer-container .about p {
 +
  margin-right:2em
 +
}
 +
.footer-container .external {
 +
  width:30%;
 +
  float:left;
 +
  text-align:right
 +
}
 +
}
 +
@media only screen and (min-width:740px) {
 +
.footer-container footer,.header-container header,.main {
 +
  width:660px;
 +
  margin:0 auto
 +
}
 +
}
 +
.clearfix:after,.clearfix:before {
 +
content:" ";
 +
display:table
 +
}
 +
.hidden {
 +
display: none;
 +
}
  
 +
</style>
 +
<style type="text/css">
 +
    /*! normalize.css v3.0.2 | MIT License | git.io/normalize */html {
 +
font-family:sans-serif;
 +
-ms-text-size-adjust:100%;
 +
-webkit-text-size-adjust:100%
 +
}
 +
body {
 +
margin:0
 +
}
 +
article,aside,details,figcaption,figure,footer,header,hgroup,main,menu,section,summary {
 +
display:block
 +
}
 +
audio,canvas,progress,video {
 +
display:inline-block;
 +
vertical-align:baseline
 +
}
 +
audio:not([controls]) {
 +
display:none;
 +
height:0
 +
}
 +
[hidden],template {
 +
display:none
 +
}
 +
a {
 +
background-color:transparent
 +
}
 +
a:active,a:hover {
 +
outline:0
 +
}
 +
abbr[title] {
 +
border-bottom:1px dotted
 +
}
 +
b,strong {
 +
font-weight:700
 +
}
 +
dfn {
 +
font-style:italic
 +
}
 +
h1 {
 +
font-size:2em;
 +
margin:.67em 0
 +
}
 +
mark {
 +
background:#ff0;
 +
color:#000
 +
}
 +
small {
 +
font-size:80%
 +
}
 +
sub,sup {
 +
font-size:75%;
 +
line-height:0;
 +
position:relative;
 +
vertical-align:baseline
 +
}
 +
sup {
 +
top:-.5em
 +
}
 +
sub {
 +
bottom:-.25em
 +
}
 +
img {
 +
border:0
 +
}
 +
svg:not(:root) {
 +
overflow:hidden
 +
}
 +
figure {
 +
margin:1em 40px
 +
}
 +
hr {
 +
-moz-box-sizing:content-box;
 +
box-sizing:content-box;
 +
height:0
 +
}
 +
pre {
 +
overflow:auto
 +
}
 +
code,kbd,pre,samp {
 +
font-family:monospace,monospace;
 +
font-size:1em
 +
}
 +
button,input,optgroup,select,textarea {
 +
color:inherit;
 +
font:inherit;
 +
margin:0
 +
}
 +
button {
 +
overflow:visible
 +
}
 +
button,select {
 +
text-transform:none
 +
}
 +
button,html input[type=button],input[type=reset],input[type=submit] {
 +
-webkit-appearance:button;
 +
cursor:pointer
 +
}
 +
button[disabled],html input[disabled] {
 +
cursor:default
 +
}
 +
button::-moz-focus-inner,input::-moz-focus-inner {
 +
border:0;
 +
padding:0
 +
}
 +
input {
 +
line-height:normal
 +
}
 +
input[type=checkbox],input[type=radio] {
 +
box-sizing:border-box;
 +
padding:0
 +
}
 +
input[type=number]::-webkit-inner-spin-button,input[type=number]::-webkit-outer-spin-button {
 +
height:auto
 +
}
 +
input[type=search] {
 +
-webkit-appearance:textfield;
 +
-moz-box-sizing:content-box;
 +
-webkit-box-sizing:content-box;
 +
box-sizing:content-box
 +
}
 +
input[type=search]::-webkit-search-cancel-button,input[type=search]::-webkit-search-decoration {
 +
-webkit-appearance:none
 +
}
 +
fieldset {
 +
border:1px solid silver;
 +
margin:0 2px;
 +
padding:.35em .625em .75em
 +
}
 +
legend {
 +
border:0;
 +
padding:0
 +
}
 +
textarea {
 +
overflow:auto
 +
}
 +
optgroup {
 +
font-weight:700
 +
}
 +
table {
 +
border-collapse:collapse;
 +
border-spacing:0
 +
}
 +
td,th {
 +
padding:0
 +
}
  
 +
</style>
  
−
<div class="column full_size">
+
    <meta charset="utf-8">
 +
    <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
 +
    <meta name="description" content="Rob Bell's software development blog, discussing object-oriented programming, design and best practices, amongst other things.">
 +
    <meta name="viewport" content="width=device-width, initial-scale=1.0">
 +
        <title>
 +
    A beginner&#39;s guide to Big O notation
 +
- Rob Bell</title>
 +
    <link rel="apple-touch-icon" href="apple-touch-icon.png">
 +
    <script type="text/javascript">
 +
      var appInsights=window.appInsights||function(config){
 +
        function i(config){t[config]=function(){var i=arguments;t.queue.push(function(){t[config].apply(t,i)})}}var t={config:config},u=document,e=window,o="script",s="AuthenticatedUserContext",h="start",c="stop",l="Track",a=l+"Event",v=l+"Page",y=u.createElement(o),r,f;y.src=config.url||"https://az416426.vo.msecnd.net/scripts/a/ai.0.js";u.getElementsByTagName(o)[0].parentNode.appendChild(y);try{t.cookie=u.cookie}catch(p){}for(t.queue=[],t.version="1.0",r=["Event","Exception","Metric","PageView","Trace","Dependency"];r.length;)i("track"+r.pop());return i("set"+s),i("clear"+s),i(h+a),i(c+a),i(h+v),i(c+v),i("flush"),config.disableExceptionTracking||(r="onerror",i("_"+r),f=e[r],e[r]=function(config,i,u,e,o){var s=f&&f(config,i,u,e,o);return s!==!0&&t["_"+r](config,i,u,e,o),s}),t
 +
        }({
 +
            instrumentationKey:"aeb013ed-162a-4cc2-bfc5-db02f3318fab"
 +
        });
  
−
<h1>Parts</h1>
+
        window.appInsights=appInsights;
 +
        appInsights.trackPageView();
 +
    </script>
 +
</head>
 +
<body>
 +
    <div class="header-container clearfix">
 +
        <header>
 +
            <h2 class="title"><a href="/">Rob Bell</a></h2>
 +
            <nav>
 +
                <ul>
 +
                    <li><a href="#1">FRET Donor</a></li>
 +
                    <li><a href="#2">FRET Acceptor</a></li>
 +
                    <li><a href="#3">Enzyme Part 00k1</a></li>
 +
                    <li><a href="#4">pH resistance Ecoli</a></li>
 +
                    <li><a href="#5">HIPIP Ecoli</a></li>
 +
                </ul>
 +
            </nav>
 +
        </header>
 +
    </div>
  
−
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
    <div class="main-container">
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
        <div class="main clearfix">
 +
           
  
 +
<article>
 +
    <header>
  
−
</div>
 
  
 +
                    <h1>Parts</h1>
  
  
  
 +
                    <p></p>
  
−
<div class="column half_size">
 
−
<div class="highlight">
 
−
<h5>Note</h5>
 
−
<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
 
−
</div>
 
−
</div>
 
  
 +
                </header>
  
  
 +
                <section>
  
−
<div class="column half_size">
 
  
−
<h5>Adding parts to the registry</h5>
+
                    <p></p>
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
+
−
</div>
+
  
  
  
 +
                    <p></p>
  
  
−
<div class="column half_size">
+
                </section>
  
−
<h5>What information do I need to start putting my parts on the Registry?</h5>
 
−
<p>The information needed to initially create a part on the Registry is:</p>
 
−
<ul>
 
−
<li>Part Name</li>
 
−
<li>Part type</li>
 
−
<li>Creator</li>
 
−
<li>Sequence</li>
 
−
<li>Short Description (60 characters on what the DNA does)</li>
 
−
<li>Long Description (Longer description of what the DNA does)</li>
 
−
<li>Design considerations</li>
 
−
</ul>
 
  
−
<p>
+
                <section id="1">
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
+
  
−
</div>
 
  
 +
                    <h2>NowGFP-golB (FRET DONOR)</h2>
  
−
<div class="column half_size">
+
                    <img src="https://static.igem.org/mediawiki/2017/4/4a/T--AshesiGhana--fretdonor.png" alt="Mountain View" >
  
−
<h5>Inspiration</h5>
+
                    <p>This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.</p>
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
  
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
−
<ul>
 
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
−
</ul>
 
−
</div>
 
  
−
<div class="column full_size">
 
−
<h5>Part Table </h5>
 
  
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
                    <pre></pre>
  
−
<div class="highlight">
 
  
 +
                </section>
  
−
</html>
 
−
<groupparts>iGEM17 AshesiGhana</groupparts>
 
  
−
<html>
+
                <section id="2">
−
</div>
+
−
</div>
+
  
  
 +
                  <h2>mRuby2-golB (FRET ACCEPTOR)</h2>
  
 +
                    <img src="https://static.igem.org/mediawiki/2017/4/4f/T--AshesiGhana--fretacceptor.png" alt="Mountain View" >
  
 +
                    <p>This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.</p>
 +
 +
 +
 +
                    <pre></pre>
 +
 +
 +
 +
                </section>
 +
 +
 +
                <section id="3">
 +
 +
 +
                    <h2>Enzyme Part 00k1</h2>
 +
 +
                    <img src="https://static.igem.org/mediawiki/2017/5/55/T--AshesiGhana--enzymepart1.png" alt="Mountain View" >
 +
 +
                    <p>To aid in the gold liberation process this part has a Tetrathionate Hydrolase gene which was mimicked from the Acidithiobacillus ferrooxidans into the E.coli strain, to function in sulphur and iron oxidizing, a process used by the Acidithiobacillus ferrooxidans in the process of gold liberation. With its own constitutive promoter, RBS and terminator this part functions as one important part in the gold liberation goal of this project.</p>
 +
 +
 +
 +
                    <pre></pre>
 +
 +
                <section id="4">
 +
 +
 +
                      <h2> pH resistance Ecoli</h2>
 +
 +
                    <img src="https://static.igem.org/mediawiki/2017/4/47/T--AshesiGhana--phresistecoli.png" alt="Mountain View" >
 +
 +
                    <p>This part is a composite part composed of a pH_resistance gene. With its own constitutive promoter, RBS and a double terminator, the part is designed for the E.coli to provide pH resistance to enable it to survive in the bio-mining environment</p>
 +
 +
 +
 +
                    <pre></pre>
 +
                </section>
 +
 +
                <section id="5">
 +
 +
 +
                      <h2>HIPIP Ecoli</h2>
 +
 +
                    <img src="https://static.igem.org/mediawiki/2017/1/1a/T--AshesiGhana--hipipecoli.png" alt="Mountain View" >
 +
 +
                    <p>This part is composed of a High potential iron-sulphur protein (HiPIP) with its own constitutive Promoter, RBS and double terminator. This part in addition to the Tetrathionate Hydrolase part enable the bacteria (E.coli) to liberate the gold from its ore similar to the way the Acidithiobacillus Ferrooxidans achieves this goal.</p>
 +
 +
 +
 +
                    <pre></pre>
 +
                </section>
 +
 +
   
 +
 +
 +
</article>
 +
 +
 +
 +
        </div>
 +
    </div>
 +
 +
    <script>
 +
        (function (i, s, o, g, r, a, m) {
 +
            i['GoogleAnalyticsObject'] = r; i[r] = i[r] || function () {
 +
                (i[r].q = i[r].q || []).push(arguments)
 +
            }, i[r].l = 1 * new Date(); a = s.createElement(o),
 +
            m = s.getElementsByTagName(o)[0]; a.async = 1; a.src = g; m.parentNode.insertBefore(a, m)
 +
        })(window, document, 'script', '//www.google-analytics.com/analytics.js', 'ga');
 +
 +
        ga('create', 'UA-412135-2', 'auto');
 +
        ga('send', 'pageview');
 +
    </script>
 +
</body>
 
</html>
 
</html>

Revision as of 10:37, 5 September 2017

Ashesi University
College

A beginner's guide to Big O notation - Rob Bell

Rob Bell

Parts

NowGFP-golB (FRET DONOR)

Mountain View

This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.




                



                



                   
mRuby2-golB (FRET ACCEPTOR)


                    Mountain View

                    
This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.




                    




                



                



                    
Enzyme Part 00k1


                    Mountain View

                    
To aid in the gold liberation process this part has a Tetrathionate Hydrolase gene which was mimicked from the Acidithiobacillus ferrooxidans into the E.coli strain, to function in sulphur and iron oxidizing, a process used by the Acidithiobacillus ferrooxidans in the process of gold liberation. With its own constitutive promoter, RBS and terminator this part functions as one important part in the gold liberation goal of this project.




                    


                



                      
 pH resistance Ecoli


                    Mountain View

                    
This part is a composite part composed of a pH_resistance gene. With its own constitutive promoter, RBS and a double terminator, the part is designed for the E.coli to provide pH resistance to enable it to survive in the bio-mining environment




                    

                


                



                      
HIPIP Ecoli


                    Mountain View

                    
This part is composed of a High potential iron-sulphur protein (HiPIP) with its own constitutive Promoter, RBS and double terminator. This part in addition to the Tetrathionate Hydrolase part enable the bacteria (E.coli) to liberate the gold from its ore similar to the way the Acidithiobacillus Ferrooxidans achieves this goal.