Difference between revisions of "Team:AshesiGhana/Parts"

Line 1: Line 1:
 
{{Template:AshesiGhana/NavBar}}
 
{{Template:AshesiGhana/NavBar}}
−
<html class="no-js">
 
−
<head>
 
−
<meta charset="utf-8">
 
−
<meta http-equiv="X-UA-Compatible" content="IE=edge">
 
  
−
<meta property="og:title" content=""/>
+
<html lang="en-GB">
−
<meta property="og:image" content=""/>
+
<head>
−
<meta property="og:url" content=""/>
+
    <link href='//fonts.googleapis.com/css?family=Raleway%7CMerriweather%7CPT+Mono' rel='stylesheet' type='text/css'>
−
<meta property="og:site_name" content=""/>
+
−
<meta property="og:description" content=""/>
+
−
<meta name="twitter:title" content="" />
+
−
<meta name="twitter:image" content="" />
+
−
<meta name="twitter:url" content="" />
+
−
<meta name="twitter:card" content="" />
+
  
−
       
+
    <style>
−
<!-- Place favicon.ico and apple-touch-icon.png in the root directory -->
+
a,nav a {
−
<link rel="shortcut icon" href="favicon.ico">
+
  text-decoration:none
−
 
+
−
<!-- Google Webfonts -->
+
−
<link href='http://fonts.googleapis.com/css?family=Roboto:400,300,100,500' rel='stylesheet' type='text/css'>
+
−
<link href='https://fonts.googleapis.com/css?family=Montserrat:400,700' rel='stylesheet' type='text/css'>
+
−
<link href='https://fonts.googleapis.com/css?family=Sanchez:400,400italic' rel='stylesheet' type='text/css'>
+
−
<link href='https://fonts.googleapis.com/css?family=Playfair+Display:400,400italic' rel='stylesheet' type='text/css'>
+
−
       
+
−
       
+
−
<style>
+
−
#home_logo, #sideMenu { display:none; }
+
−
#sideMenu, #top_title, .patrollink {display:none;}
+
−
#content { width:100%; padding:0px;  margin-top:-13px; margin-left:0px;}
+
−
body {background-color:white; }
+
−
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
+
−
 
+
−
.navbar-brand {
+
−
  padding: 0px;
+
 
}
 
}
−
.navbar-brand>img {
+
.comments div,.meta,code,pre {
−
  height: 100%;
+
font-size:.9em
−
  padding: 15px;
+
−
  width: auto;
+
 
}
 
}
−
 
+
.clearfix:after,.footer-container footer>p {
−
.navigation {
+
clear:both
−
    height: 70px;
+
}
−
    margin-top: 10px;
+
img {
−
    padding: 0;
+
vertical-align:middle
−
    background: white;
+
}
−
    font-size: 70%;
+
fieldset {
 +
border:0;
 +
margin:0;
 +
padding:0
 +
}
 +
textarea {
 +
resize:vertical
 +
}
 +
::-moz-selection {
 +
background:#C8E6FD;
 +
text-shadow:none
 +
}
 +
::selection {
 +
background:#C8E6FD;
 +
text-shadow:none
 +
}
 +
body {
 +
background:#fff;
 +
font:1em/2 Merriweather,Georgia,Times,serif;
 +
color:#555
 +
}
 +
a {
 +
color:#494B4B;
 +
border-bottom:1px solid #02BCDC;
 +
-o-transition:.25s;
 +
-ms-transition:.25s;
 +
-moz-transition:.25s;
 +
-webkit-transition:.25s;
 +
transition:.25s
 +
}
 +
a:hover {
 +
color:#02BCDC
 +
}
 +
h1,h2,h3,h4,h5,h6 {
 +
font-weight:400;
 +
font-family:Raleway,Arial,sans-serif;
 +
line-height:1.5em;
 +
color:#666
 +
}
 +
.header-container {
 +
margin:1em 1em 0
 +
}
 +
.title a {
 +
width:181px;
 +
height:51px;
 +
border:0;
 +
text-indent:-9999px;
 +
display:block;
 +
background-image:url(../img/site-name.png);
 +
background-image:-webkit-image-set(url(../img/site-name.png) 1x,url(../img/site-name-2x.png) 2x);
 +
background-position:left center;
 +
background-repeat:no-repeat
 +
}
 +
nav a {
 +
font-family:Raleway,Arial,Sans-Serif;
 +
color:#333;
 +
border:0;
 +
display:inline-block
 +
}
 +
nav ul {
 +
list-style-type:none;
 +
padding-left:0;
 +
margin-left:-1em
 +
}
 +
nav li {
 +
display:inline;
 +
margin-left:1em
 +
}
 +
.main-container {
 +
margin:0 1em 2em
 +
}
 +
code,pre {
 +
font-family:"PT Mono","Courier New",Courier,monospace;
 +
color:#D50085
 +
}
 +
pre {
 +
line-height:1.5em;
 +
margin:1.1em 0
 +
}
 +
article:not(:last-child) {
 +
margin-bottom:2em
 +
}
 +
.meta {
 +
background:#EDF0F3;
 +
padding:.5em 1em
 +
}
 +
.meta h4,.meta p {
 +
display:table-cell;
 +
line-height:2em;
 +
margin:0
 +
}
 +
.meta h4 {
 +
float:left;
 +
margin-right:.5em
 +
}
 +
.comments div {
 +
background:#E9F5F8;
 +
padding:.5em 1em;
 +
margin:1em 0;
 +
word-wrap:break-word
 +
}
 +
.comments div:nth-child(odd) {
 +
background:url(../img/comment-left-bg.png)bottom left no-repeat #E2F2FE;
 +
padding-left:2em
 +
}
 +
.comments div:nth-child(even) {
 +
background:url(../img/comment-right-bg.png)bottom right no-repeat #E2F2FE;
 +
padding-right:2em
 +
}
 +
.comments h4,.comments p {
 +
margin:.5em 0
 +
}
 +
.comments p {
 +
line-height:1.5em
 +
}
 +
.comments h4 {
 +
line-height:1em;
 +
margin-bottom:1em
 +
}
 +
.footer-container {
 +
margin:1em
 +
}
 +
.footer-container footer {
 +
padding-top:1em;
 +
border-top:1px dashed #70828F
 +
}
 +
.footer-container ul {
 +
list-style-type:none;
 +
padding-left:0
 +
}
 +
.footer-container li {
 +
display:inline
 +
}
 +
.footer-container a {
 +
display:inline-block;
 +
background:#366485;
 +
color:#eee;
 +
border:0;
 +
padding:.3em .5em;
 +
margin-bottom:.3em;
 +
line-height:1.2em
 +
}
 +
.footer-container a:hover {
 +
background:#4089BF
 +
}
 +
@media only screen and (min-width:620px) {
 +
.header-container {
 +
  margin:2em 2em 0
 +
}
 +
.header-container .title {
 +
  float:left
 +
}
 +
nav {
 +
  float:right;
 +
  line-height:3.6em
 +
}
 +
.main-container {
 +
  margin:0 2em 2em
 +
}
 +
.meta .category,.meta .date {
 +
  width:25%
 +
}
 +
.meta .tags {
 +
  width:50%
 +
}
 +
.meta div {
 +
  float:left
 +
}
 +
.meta h4,.meta p {
 +
  float:none;
 +
  display:block
 +
}
 +
.footer-container {
 +
  margin:1em 2em
 +
}
 +
.footer-container li {
 +
  display:block
 +
}
 +
.footer-container .about {
 +
  width:70%;
 +
  float:left
 +
}
 +
.footer-container .about h4,.footer-container .about p {
 +
  margin-right:2em
 +
}
 +
.footer-container .external {
 +
  width:30%;
 +
  float:left;
 +
  text-align:right
 +
}
 +
}
 +
@media only screen and (min-width:740px) {
 +
.footer-container footer,.header-container header,.main {
 +
  width:660px;
 +
  margin:0 auto
 +
}
 +
}
 +
.clearfix:after,.clearfix:before {
 +
content:" ";
 +
display:table
 +
}
 +
.hidden {
 +
display: none;
 
}
 
}
  
−
.brand{
+
</style>
−
color:black;  
+
<style type="text/css">
 +
    /*! normalize.css v3.0.2 | MIT License | git.io/normalize */html {
 +
font-family:sans-serif;
 +
-ms-text-size-adjust:100%;
 +
-webkit-text-size-adjust:100%
 +
}
 +
body {
 +
margin:0
 +
}
 +
article,aside,details,figcaption,figure,footer,header,hgroup,main,menu,section,summary {
 +
display:block
 +
}
 +
audio,canvas,progress,video {
 +
display:inline-block;
 +
vertical-align:baseline
 +
}
 +
audio:not([controls]) {
 +
display:none;
 +
height:0
 +
}
 +
[hidden],template {
 +
display:none
 +
}
 +
a {
 +
background-color:transparent
 +
}
 +
a:active,a:hover {
 +
outline:0
 +
}
 +
abbr[title] {
 +
border-bottom:1px dotted
 +
}
 +
b,strong {
 +
font-weight:700
 +
}
 +
dfn {
 +
font-style:italic
 +
}
 +
h1 {
 +
font-size:2em;
 +
margin:.67em 0
 +
}
 +
mark {
 +
background:#ff0;
 +
color:#000
 +
}
 +
small {
 +
font-size:80%
 +
}
 +
sub,sup {
 +
font-size:75%;
 +
line-height:0;
 +
position:relative;
 +
vertical-align:baseline
 +
}
 +
sup {
 +
top:-.5em
 +
}
 +
sub {
 +
bottom:-.25em
 +
}
 +
img {
 +
border:0
 +
}
 +
svg:not(:root) {
 +
overflow:hidden
 +
}
 +
figure {
 +
margin:1em 40px
 +
}
 +
hr {
 +
-moz-box-sizing:content-box;
 +
box-sizing:content-box;
 +
height:0
 +
}
 +
pre {
 +
overflow:auto
 +
}
 +
code,kbd,pre,samp {
 +
font-family:monospace,monospace;
 +
font-size:1em
 +
}
 +
button,input,optgroup,select,textarea {
 +
color:inherit;
 +
font:inherit;
 +
margin:0
 +
}
 +
button {
 +
overflow:visible
 +
}
 +
button,select {
 +
text-transform:none
 +
}
 +
button,html input[type=button],input[type=reset],input[type=submit] {
 +
-webkit-appearance:button;
 +
cursor:pointer
 +
}
 +
button[disabled],html input[disabled] {
 +
cursor:default
 +
}
 +
button::-moz-focus-inner,input::-moz-focus-inner {
 +
border:0;
 +
padding:0
 +
}
 +
input {
 +
line-height:normal
 +
}
 +
input[type=checkbox],input[type=radio] {
 +
box-sizing:border-box;
 +
padding:0
 +
}
 +
input[type=number]::-webkit-inner-spin-button,input[type=number]::-webkit-outer-spin-button {
 +
height:auto
 +
}
 +
input[type=search] {
 +
-webkit-appearance:textfield;
 +
-moz-box-sizing:content-box;
 +
-webkit-box-sizing:content-box;
 +
box-sizing:content-box
 +
}
 +
input[type=search]::-webkit-search-cancel-button,input[type=search]::-webkit-search-decoration {
 +
-webkit-appearance:none
 +
}
 +
fieldset {
 +
border:1px solid silver;
 +
margin:0 2px;
 +
padding:.35em .625em .75em
 +
}
 +
legend {
 +
border:0;
 +
padding:0
 +
}
 +
textarea {
 +
overflow:auto
 +
}
 +
optgroup {
 +
font-weight:700
 +
}
 +
table {
 +
border-collapse:collapse;
 +
border-spacing:0
 +
}
 +
td,th {
 +
padding:0
 
}
 
}
  
 +
</style>
  
 +
    <meta charset="utf-8">
 +
    <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
 +
    <meta name="description" content="Rob Bell's software development blog, discussing object-oriented programming, design and best practices, amongst other things.">
 +
    <meta name="viewport" content="width=device-width, initial-scale=1.0">
 +
        <title>
 +
    A beginner&#39;s guide to Big O notation
 +
- Rob Bell</title>
 +
    <link rel="apple-touch-icon" href="apple-touch-icon.png">
 +
    <script type="text/javascript">
 +
      var appInsights=window.appInsights||function(config){
 +
        function i(config){t[config]=function(){var i=arguments;t.queue.push(function(){t[config].apply(t,i)})}}var t={config:config},u=document,e=window,o="script",s="AuthenticatedUserContext",h="start",c="stop",l="Track",a=l+"Event",v=l+"Page",y=u.createElement(o),r,f;y.src=config.url||"https://az416426.vo.msecnd.net/scripts/a/ai.0.js";u.getElementsByTagName(o)[0].parentNode.appendChild(y);try{t.cookie=u.cookie}catch(p){}for(t.queue=[],t.version="1.0",r=["Event","Exception","Metric","PageView","Trace","Dependency"];r.length;)i("track"+r.pop());return i("set"+s),i("clear"+s),i(h+a),i(c+a),i(h+v),i(c+v),i("flush"),config.disableExceptionTracking||(r="onerror",i("_"+r),f=e[r],e[r]=function(config,i,u,e,o){var s=f&&f(config,i,u,e,o);return s!==!0&&t["_"+r](config,i,u,e,o),s}),t
 +
        }({
 +
            instrumentationKey:"aeb013ed-162a-4cc2-bfc5-db02f3318fab"
 +
        });
  
 +
        window.appInsights=appInsights;
 +
        appInsights.trackPageView();
 +
    </script>
 +
</head>
 +
<body>
 +
    <div class="header-container clearfix">
 +
        <header>
 +
            <h2 class="title"><a href="/">Rob Bell</a></h2>
 +
            <nav>
 +
                <ul>
 +
                    <li><a href="#1">FRET Donor</a></li>
 +
                    <li><a href="#2">FRET Acceptor</a></li>
 +
                    <li><a href="#3">Enzyme Part 00k1</a></li>
 +
                    <li><a href="#4">pH resistance Ecoli</a></li>
 +
                    <li><a href="#5">HIPIP Ecoli</a></li>
 +
                </ul>
 +
            </nav>
 +
        </header>
 +
    </div>
  
 +
    <div class="main-container">
 +
        <div class="main clearfix">
 +
           
  
−
/*************************
+
<article>
−
EXAMPLES 2-7 BELOW
+
    <header>
−
**************************/
+
  
−
/* EXAMPLE 2 (larger logo) - simply adjust top bottom padding to make logo larger */
 
  
−
.example2 .navbar-brand>img {
+
                    <h1>Parts</h1>
−
  padding: 7px 15px;
+
−
}
+
  
  
−
/* EXAMPLE 3
 
  
−
line height is 20px by default so add 30px top and bottom to equal the new .navbar-brand 80px height  */
+
                    <p></p>
  
−
.example3 .navbar-brand {
 
−
  height: 80px;
 
−
}
 
  
−
.example3 .nav >li >a {
+
                </header>
−
  padding-top: 30px;
+
−
  padding-bottom: 30px;
+
−
}
+
−
.example3 .navbar-toggle {
+
−
  padding: 10px;
+
−
  margin: 25px 15px 25px 0;
+
−
}
+
  
  
−
/* EXAMPLE 4 - Small Narrow Logo*/
+
                <section>
−
.example4 .navbar-brand>img {
+
−
  padding: 7px 14px;
+
−
}
+
  
  
−
/* EXAMPLE 5 - Logo with Text*/
+
                    <p></p>
−
.example5 .navbar-brand {
+
−
  display: flex;
+
−
  align-items: center;
+
−
}
+
−
.example5 .navbar-brand>img {
+
−
  padding: 7px 14px;
+
−
}
+
  
  
−
/* EXAMPLE 6 - Background Logo*/
 
−
.example6 .navbar-brand{
 
−
  background: url(https://res.cloudinary.com/candidbusiness/image/upload/v1455406304/dispute-bills-chicago.png) center / contain no-repeat;
 
−
  width: 200px;
 
−
}
 
  
 +
                    <p></p>
  
  
 +
                </section>
  
  
−
/* EXAMPLE 8 - Center on mobile*/
+
                <section id="1">
−
@media only screen and (max-width : 768px){
+
−
  .example-8 .navbar-brand {
+
−
  padding: 0px;
+
−
  transform: translateX(-50%);
+
−
  left: 50%;
+
−
  position: absolute;
+
−
}
+
−
.example-8 .navbar-brand>img {
+
−
  height: 100%;
+
−
  width: auto;
+
−
  padding: 7px 14px;
+
−
}
+
−
}
+
  
  
−
/* EXAMPLE 8 - Center Background */
+
                    <h2>NowGFP-golB (FRET DONOR)</h2>
−
.example-8 .navbar-brand {
+
−
  background: url(https://res.cloudinary.com/candidbusiness/image/upload/v1455406304/dispute-bills-chicago.png) center / contain no-repeat;
+
−
  width: 200px;
+
−
  transform: translateX(-50%);
+
−
  left: 50%;
+
−
  position: absolute;
+
−
}
+
  
 +
                    <img src="https://static.igem.org/mediawiki/2017/4/4a/T--AshesiGhana--fretdonor.png" alt="Mountain View" >
  
−
/* EXAMPLE 9 - Center with Flexbox and Text*/
+
                    <p>This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.</p>
−
.brand-centered {
+
−
  display: flex;
+
−
  justify-content: center;
+
−
  position: absolute;
+
−
  width: 100%;
+
−
  left: 0;
+
−
  top: 0;
+
−
}
+
−
.brand-centered .navbar-brand {
+
−
  display: flex;
+
−
  align-items: center;
+
−
}
+
−
.navbar-toggle {
+
−
    z-index: 1;
+
−
}
+
  
−
/* CSS Transform Align Navbar Brand Text ... This could also be achieved with table / table-cells */
 
−
.navbar-alignit .navbar-header {
 
−
  -webkit-transform-style: preserve-3d;
 
−
  -moz-transform-style: preserve-3d;
 
−
  transform-style: preserve-3d;
 
−
  height: 50px;
 
−
}
 
−
.navbar-alignit .navbar-brand {
 
−
top: 50%;
 
−
display: block;
 
−
position: relative;
 
−
height: auto;
 
−
transform: translate(0,-50%);
 
−
margin-right: 15px;
 
−
  margin-left: 15px;
 
−
}
 
  
−
.navbar-nav>li>.dropdown-menu {
 
−
z-index: 9999;
 
−
}
 
  
−
body {
+
                    <pre></pre>
−
  font-family: "Lato";
+
−
}
+
−
</style>
+
−
+
  
−
<!-- Animate.css -->
 
−
<!-- <link rel="stylesheet" href="https://ashbuspay.000webhostapp.com/animate.css"> -->
 
−
<link rel="stylesheet" type="text/css"
 
−
href="https://2017.igem.org/Template:AshesiGhana/CSS:animate?action=raw&ctype=text/css" />
 
−
<!-- Icomoon Icon Fonts-->
 
−
<!-- <link rel="stylesheet" href="https://ashbuspay.000webhostapp.com/icomoon.css"> -->
 
  
−
<link rel="stylesheet" type="text/css"
+
                </section>
−
href="https://2017.igem.org/Template:AshesiGhana/CSS:icomoon?action=raw&ctype=text/css" />
+
−
<!-- Magnific Popup -->
+
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                  <h2>mRuby2-golB (FRET ACCEPTOR)</h2>
  
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                    <p>This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.</p>
  
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<h1 class="fh5co-intro-lead animate-1 to-animate">Team AshesiGhana</h1>
 
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<h2 class="fh5co-intro-sub animate-2 to-animate">Introducing the Greatest Gold Miners in the World....Microbes</h2>
 
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<p>For the 2017 IGEM, Team AshesiGhana is working on a bio-mining project. The objective of our project is to develop an organism capable of liberating gold from the refractory ore. We aim to provide not only an alternative, non-toxic approach for small scale mining, but also deliver an easy and fast bio-detection and quantification method of ore in the mining industry of our country. The availability of such a biosensor will allow routine monitoring of the ore before a mining endeavor is undertaken thus preventing the destruction of the environment. The project will engineer the typical environmental organism Acidithiobacillus ferroxidans with a FRET probe.</p>
 
  
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<p>This new organism will primarily be capable of sensing and quantifying the amount of gold in the ore. This will be accomplished by using a two-part probe, a donor part which is made up of gold binding protein (golB) attached to a green fluorescent protein (nowGFP). The second part of the acceptor is also made up of a binding protein and a red fluorescent protein (mRuby2). In the presence of a high amount of free gold, the two parts would be in close proximity and energy transfer can take place and the red protein would be excited giving off a fluorescent signal. Using calibration experiments, we can relate the amount of fluorescence to the amount of gold present,liberated by the organism from the ore.</p>
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<p>As Acidithiobacillus ferroxidans is a difficult organism to grow in large quantities, we will engineer a strain of E coli to produce two of the main oxidizing enzymes for iron and sulphite which will liberate the gold from the ore. The organism will also be engineered with protective enzymes against the low pH, which is one of the bi-products of the gold liberation reaction, and metal toxicity. The same FRET biosensor part can be added to the E coli strain for the quantification of gold. This new organism can easily be grown in large batches and so can be used to extract gold from refractory ore without the need of any toxic treatment thus providing a safe alternative for small scale mining. </p>
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                    <h2>Enzyme Part 00k1</h2>
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                    <p>To aid in the gold liberation process this part has a Tetrathionate Hydrolase gene which was mimicked from the Acidithiobacillus ferrooxidans into the E.coli strain, to function in sulphur and iron oxidizing, a process used by the Acidithiobacillus ferrooxidans in the process of gold liberation. With its own constitutive promoter, RBS and terminator this part functions as one important part in the gold liberation goal of this project.</p>
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                      <h2> pH resistance Ecoli</h2>
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                    <p>This part is a composite part composed of a pH_resistance gene. With its own constitutive promoter, RBS and a double terminator, the part is designed for the E.coli to provide pH resistance to enable it to survive in the bio-mining environment</p>
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                      <h2>HIPIP Ecoli</h2>
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                    <p>This part is composed of a High potential iron-sulphur protein (HiPIP) with its own constitutive Promoter, RBS and double terminator. This part in addition to the Tetrathionate Hydrolase part enable the bacteria (E.coli) to liberate the gold from its ore similar to the way the Acidithiobacillus Ferrooxidans achieves this goal.</p>
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Revision as of 15:12, 24 September 2017

Ashesi University
College

A beginner's guide to Big O notation - Rob Bell

Rob Bell

Parts

NowGFP-golB (FRET DONOR)

Mountain View

This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.




                



                



                   
mRuby2-golB (FRET ACCEPTOR)


                    Mountain View

                    
This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.




                    




                



                



                    
Enzyme Part 00k1


                    Mountain View

                    
To aid in the gold liberation process this part has a Tetrathionate Hydrolase gene which was mimicked from the Acidithiobacillus ferrooxidans into the E.coli strain, to function in sulphur and iron oxidizing, a process used by the Acidithiobacillus ferrooxidans in the process of gold liberation. With its own constitutive promoter, RBS and terminator this part functions as one important part in the gold liberation goal of this project.




                    


                



                      
 pH resistance Ecoli


                    Mountain View

                    
This part is a composite part composed of a pH_resistance gene. With its own constitutive promoter, RBS and a double terminator, the part is designed for the E.coli to provide pH resistance to enable it to survive in the bio-mining environment




                    

                


                



                      
HIPIP Ecoli


                    Mountain View

                    
This part is composed of a High potential iron-sulphur protein (HiPIP) with its own constitutive Promoter, RBS and double terminator. This part in addition to the Tetrathionate Hydrolase part enable the bacteria (E.coli) to liberate the gold from its ore similar to the way the Acidithiobacillus Ferrooxidans achieves this goal.