Difference between revisions of "Team:Heidelberg/Education"

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           <div class="header-title heidelberg-red">Education</div>
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           <div class="header-subtitle" style="color: #393939 !important;">Engaging with the public</div>
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The High School <a href="https://fro-ev.de">Research Lab Ortenau</a> is a place for young scientists to get in touch with biological research and to gain wet-lab experience. We decided to closely collaborate with the High School Research lab during the 2017 iGEM season. The Research Lab Ortenau aims at arousing the student’s interest for biology. Mentoring the High School students meant great responsibility for us, and provided the opportunity to be involved even more in the synthetic biology community. We even intensified the collaboration, when Tobias Stadelmann, a high school student from the Research Lab Ortenau, asked whether it would be possible to work in our iGEM lab for a two-week internship. In the High School Research Lab Tobias is working on a project based on DNAzmes. During the time in our iGEM lab, Tobias was involved in our experiments to simplify PACE and to establish PREDCEL for directed evolution approaches. We are sure that all of us, Tobias, the High School Research Lab Ortenau, and the iGEM team gained profit from this collaboration. We are confident, that Tobias can apply the newly learned techniques to his DNazymes project and that he can transfer the know-how to other projects in the High School Research Lab Ortenau.
  
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Opening science to a broad public is a major goal in iGEM. To <a href="https://2017.igem.org/Team:Heidelberg/Engagement#Public_Survey_PP">integrate public opinions and concerns</a> into the scientific process is increasingly important as new biological inventions could influence the humanity as a whole, the environment and our coexistence with nature. Therefore, we did not want to miss the opportunity to host <a href="https://2017.igem.org/Team:Heidelberg/Interviews#Esvelt_expert_PP">Kevin Esvelt</a>, the inventor of PACE (phage-assisted continuous evolution) and CRISPR-based gene drives, for an open lecture at our university. Kevin Esvelt presented his work on "Gene drive evolution, localization, and restoration". This highly intriguing topic and he, being the speaker, attracted more than 300 students and researchers with various different backgrounds. The concept of gene drives already exists in nature and allows particular genes to spread through populations even if those genes are not necessarily beneficial for an organism´s fitness. Kevin Esvelt and his colleagues mimicked this naturally occurring concept using the CRISPR/Cas technology, thereby enabling genetic alterations in sexually reproducing organisms to spread throughout populations. This way, new genes can be introduced into populations, e.g. to control reproduction of apopulation under specific circumstances. Consequently, applications of gene drives include preventing the spread of pathogen carrying insects like mosquitos transmitting malaria. As gene drives are a tool to bias inheritance of genes, the potential, enormous impact of releasing gene drive carrying organisms into nature raises major bioethical concerns. Therefore, Kevin Esvelt´s is continuously perusing an open dialog with the society about benefits and potential risks of scientific technologies before they are even started to be implemented in his lab. Notably, this aim of integrating the public opinion and discussion into the scientific process from the very beginning is also a hallmark of and key criterion for <a href="https://2017.igem.org/Team:Heidelberg/Integrated_Gold"> successful iGEM projects</a>. By giving people a voice, they also get a choice. Emerging technologies may affect countries or even our planet as a whole and the <a href="https://2017.igem.org/Team:Heidelberg/Engagement#Public_Survey_PP">broad public</a> should have a voice in which way technology should be developed and how it is to be used. Besides CRISPR-based gene drives, Kevin Esvelt is also the inventor of PACE (phage-assisted continuous evolution), the method we want to build upon in our project. We were lucky to not only host Kevin Esvelt’s lecture at the BioQuant building, but even had the opportunity to actually show him our lab (which is located in BioQuant) and PACE apparatus and discuss our iGEM project. He provided extremely helpful advice not only on how to set up PACE technically, but also on how to design corresponding experiments safely and responsibly and how to communicate our project outside the scientific community.
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If you are interested in how we integrated <a href="https://2017.igem.org/Team:Heidelberg/Interviews#Esvelt_expert_PP">Kevin Esvelt’s advice</a> into our project design, please visit our <a href="https://2017.igem.org/Team:Heidelberg/Integrated_Gold">Integrated Human Practices page</a>. We are proud of having hosted Kevin Esvelt as a speaker and guest and are thankful for his inspiring input that shaped our project from its very beginning on.
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To get in touch with people from different backgrounds, we decided to present iGEM at the TEDx event in Heidelberg. TED (Technology, Entertainment and Design) is a nonprofit organization providing a stage for "ideas worth spreading". Short powerful talks give the audience an insight into a variety of topics and TED hosted renowned speakers like Jennifer Doudna, Stephen Hawking, Craig Venter, Steve Jobs, Bill Clinton and <a href="https://2017.igem.org/Team:Heidelberg/Interviews#Adlung_expert_PP">Lorenz Adlung</a>. To illustrate how evolution is driving nature towards fascinating complexity, we presented an evolutionary game  showcasing the causality between randomly occurring mutations and beneficial phenotypes under distinct environmental conditions.
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Arousing the interest of High School students for science is a major aim we pursued during our Eduction activites. Besides the <a href=#iGEM Seminar>iGEM Seminar</a> we gave at a local high school we received a request from a high school from Berlin. They were highly interested in visiting our lab for gaining some practical experience and getting insights into the research on directed evolution we perform in our lab. We hosted this high school class for a course on responsible genetic engineering in our lab. First we gave a short lecture on evolution, gene technology and introduced the theoretical background of our project to the class. We asked them to fill in our <a href=#Survey School Berlin>Survey for High School Students</a> and they answered our questions with great passion. After they have gained enough theoretical knowledge about the experiments we perform in our lab, the attended a safety instruction held by our PI Dominik Niopek. They learned how to behave in a risk group 1 laboratory like our iGEM lab, for example that everyone needs to wear a lab coat and gloves, that food and drinks are forbidden as well as more general safety and hygiene measures like how to wash one´s hands.
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Playing the game, the conference attendees first had to roll a dice. The different sides of the dice thereby represented a specific environmental change or obstacle. For instance, a hot period, a drought or an ice age could come up, thereby causing a need for adaptation (selection pressure). Secondly, we created a wheel of evolution, each field on the wheel representing a single, potentially beneficial variation (mutant) of a protein. In other words, the wheel would represent the gene pool of a specific species. Players of our game could thereby experience how the interplay between random mutation and selection propels nature towards continuous adaptation and invention of novel functions.
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Thereby, our evolution game not only introduced the TEDx audience to the concept of Darwinian evolution. It served as starting point for further discussions about our iGEM project on in vivo directed evolution, as well as many other topics including the role of human in the reduction or even extinction of certain species (e.g. the bee population being dramatically reduced, likely due to chemicals used in agriculture), and genetically modified organisms in general. Inspiring conversations with people from various backgrounds offered us an insight into different perspectives towards our project. Surprisingly, the group of non-scientists rather unfamiliar with the details of Darwinian evolution beforehand was particularly excited about our iGEM project ideas once they had played our evolution game and started to think about evolution as a technology.
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The feedback we received was althrough positive and they left our iGEM lab with new experiences and a general impression on about every day live of scientists in the field of Synthetic Biology.
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To receive productive feedback from the TEDx visitors we asked them to write down personal concerns, comments, and desires in context of our project idea and synthetic biology in general. We collected these statements anonymously in our "Synthetic Biology Box”. The very open feedback we received from the TEDx visitors shaped our project from the very beginning. For instance, one visitor noted, that we need to make sure our in vivo directional evolution approach (named PREDCEL for phage-related discontinuous evolution) was used responsibly and for the welfare of humanity. This feedback inspired us build our <a href="https://2017.igem.org/Team:Heidelberg/Toolbox#Questionnaire_PP">"Are you ready to PREDCEL?"</a> question catalogue, which now represents the entry point to our <a href="https://2017.igem.org/Team:Heidelberg/Toolbox">toolbox</a> and aims at stimulating a responsible as well as technically educated use of our in vivo directed evolution technology. Other TEDx visitors expressed concern that researchers might unintendedly evolve dangerous or hazardous proteins. This feedback inspired us to create <a href="https://2017.igem.org/Team:Heidelberg/Software/SafetyNet">SafetyNet</a>, an online tool aiming to determine whether an input protein sequence would be safe to be used in PREDCEL. SafetyNet first performs a simple blast search and compares the results to a curated database of hazardous (e.g. pathogen-related, toxic or oncogenic) proteins. Then, SafetyNet uses artificial intelligence to infer “sleeping” hazardous potential in the input sequence, which could be accidently evoked during directed evolution in the lab. Hence, our SafetyNet enables researchers to easily test the safety of their evolution experiments prior to actually going to the lab.
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Overall, the evaluation of the anonymous TEDx visitor notes revealed that in particular the non‑scientists highly appreciated the opportunity to openly discuss a genetic engineering project prior to its actual implementation in the wet lab and to have a word in its design and setup. On the other hand, it was the open feedback by these people, that stimulated ideas on our side (<a href="https://2017.igem.org/Team:Heidelberg/Software/SafetyNet">SafetyNet</a>, <a href="https://2017.igem.org/Team:Heidelberg/Toolbox#Questionnaire_PP">"Are you ready to PREDCEL?"</a>) on how to enable the responsible and safe use of our in vivo directed evolution methods by other iGEM teams as well as outside iGEM.  
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<b>Educating Responsible Future Scientits</b>
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Educating high school students also comprises stimulating them to being reflective, responsible and communicating their personal opinions. Therefore, we created a survey for high school students that serves as a starting point for the aforementioned points. Here you find the results of our questionnaire filled in during the responsible genetic engineering course in our lab.
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As we decided to pursue a directed evolution approach in our project, we wondered how the general public would perceive our research aim. Are there concerns that we could address in our project? Did we think about all the safety aspects of our project? Moreover, the combination of directed evolution and artificial intelligence is a novel and visionary approach in interdisciplinary research. How would the general public react to the combination of those two itself controversial subjects?
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Arousing the interest of High School students for science is a major aim we pursued during our Eduction activites. Besides the <a href=#iGEM Seminar>iGEM Seminar</a> we gave at a local high school we received a request from a high school from Berlin. They were highly interested in visiting our lab for gaining some practical experience and getting insights into the research on directed evolution we perform in our lab. We hosted this high school class for a course on responsible genetic engineering in our lab. First we gave a short lecture on evolution, gene technology and introduced the theoretical background of our project to the class. We asked them to fill in our <a href=#Survey School Berlin>Survey for High School Students</a> and they answered our questions with great passion. After they have gained enough theoretical knowledge about the experiments we perform in our lab, the attended a safety instruction held by our PI Dominik Niopek. They learned how to behave in a risk group 1 laboratory like our iGEM lab, for example that everyone needs to wear a lab coat and gloves, that food and drinks are forbidden as well as more general safety and hygiene measures like how to wash one´s hands.
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The feedback we received was althrough positive and they left our iGEM lab with new experiences and a general impression on about every day live of scientists in the field of Synthetic Biology.
  
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We created a <a href="https://goo.gl/forms/eh7JoWtkaSIgn2B13">survey</a>survey to address these questions and advertised the questionnaire within the iGEM community, on social media platforms and the various open events that we organized. We reached over 100 participants at the age of 13 to 70 years, who filled in our survey. In general, the participants see and expect great benefits of directed evolution approaches. However, most of them feel the need of guidelines (55,7%) or strict regulations (29,2%) on such technologies in order to prevent intentional misuse or accidentally generated hazards. Our participants support the combination of directed evolution and artificial intelligence, as it reduces great costs for laboratory equipment and human resources. A large part of our participants support AI (67,9 %), whereas about one third (26,4%) is not sure whether to approve or disapprove of AI. With our project and the platform that iGEM offers, we hope to reach especially the people that are indecisive about the new biological advances and help them to reflect about their opinion.
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To address the concerns, which the participants of our survey and our team share, we developed our <a href="https://2017.igem.org/Team:Heidelberg/Software/SafetyNet">SafetyNet</a> that calculates possible hazardous sequences that could evolve during PACE or PREDCEL. Furthermore, we created an <a href="https://2017.igem.org/Team:Heidelberg/Toolbox#Questionnaire_PP">"Are you ready to PREDCEL?"</a>  questionnaire that anyone interested in our toolbox has to fill in before it is open to use. We hope to urge our users to engage into critical thinking and to reflect on the possible consequences of their research.
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<b>Educating Responsible Future Scientits</b>
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Educating high school students also comprises stimulating them to being reflective, responsible and communicating their personal opinions. Therefore, we created a survey for high school students that serves as a starting point for the aforementioned points. Here you find the results of our questionnaire filled in during our iGEM seminar at a local high school.
  
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Revision as of 00:32, 2 November 2017

Education
Shaping the Next Generation of Scientists


Tobias´ Internship at the iGEM lab

The High School Research Lab Ortenau is a place for young scientists to get in touch with biological research and to gain wet-lab experience. We decided to closely collaborate with the High School Research lab during the 2017 iGEM season. The Research Lab Ortenau aims at arousing the student’s interest for biology. Mentoring the High School students meant great responsibility for us, and provided the opportunity to be involved even more in the synthetic biology community. We even intensified the collaboration, when Tobias Stadelmann, a high school student from the Research Lab Ortenau, asked whether it would be possible to work in our iGEM lab for a two-week internship. In the High School Research Lab Tobias is working on a project based on DNAzmes. During the time in our iGEM lab, Tobias was involved in our experiments to simplify PACE and to establish PREDCEL for directed evolution approaches. We are sure that all of us, Tobias, the High School Research Lab Ortenau, and the iGEM team gained profit from this collaboration. We are confident, that Tobias can apply the newly learned techniques to his DNazymes project and that he can transfer the know-how to other projects in the High School Research Lab Ortenau.

Responsible Genetic Engineering Course in our Lab

Arousing the interest of High School students for science is a major aim we pursued during our Eduction activites. Besides the iGEM Seminar we gave at a local high school we received a request from a high school from Berlin. They were highly interested in visiting our lab for gaining some practical experience and getting insights into the research on directed evolution we perform in our lab. We hosted this high school class for a course on responsible genetic engineering in our lab. First we gave a short lecture on evolution, gene technology and introduced the theoretical background of our project to the class. We asked them to fill in our Survey for High School Students and they answered our questions with great passion. After they have gained enough theoretical knowledge about the experiments we perform in our lab, the attended a safety instruction held by our PI Dominik Niopek. They learned how to behave in a risk group 1 laboratory like our iGEM lab, for example that everyone needs to wear a lab coat and gloves, that food and drinks are forbidden as well as more general safety and hygiene measures like how to wash one´s hands.
The feedback we received was althrough positive and they left our iGEM lab with new experiences and a general impression on about every day live of scientists in the field of Synthetic Biology.
Educating Responsible Future Scientits
Educating high school students also comprises stimulating them to being reflective, responsible and communicating their personal opinions. Therefore, we created a survey for high school students that serves as a starting point for the aforementioned points. Here you find the results of our questionnaire filled in during the responsible genetic engineering course in our lab.

iGEM Seminar at a local High School

Arousing the interest of High School students for science is a major aim we pursued during our Eduction activites. Besides the iGEM Seminar we gave at a local high school we received a request from a high school from Berlin. They were highly interested in visiting our lab for gaining some practical experience and getting insights into the research on directed evolution we perform in our lab. We hosted this high school class for a course on responsible genetic engineering in our lab. First we gave a short lecture on evolution, gene technology and introduced the theoretical background of our project to the class. We asked them to fill in our Survey for High School Students and they answered our questions with great passion. After they have gained enough theoretical knowledge about the experiments we perform in our lab, the attended a safety instruction held by our PI Dominik Niopek. They learned how to behave in a risk group 1 laboratory like our iGEM lab, for example that everyone needs to wear a lab coat and gloves, that food and drinks are forbidden as well as more general safety and hygiene measures like how to wash one´s hands.
The feedback we received was althrough positive and they left our iGEM lab with new experiences and a general impression on about every day live of scientists in the field of Synthetic Biology.
Educating Responsible Future Scientits
Educating high school students also comprises stimulating them to being reflective, responsible and communicating their personal opinions. Therefore, we created a survey for high school students that serves as a starting point for the aforementioned points. Here you find the results of our questionnaire filled in during our iGEM seminar at a local high school.

Explanatory and Educative Video about our Project

Communicating science in a simple and easy understandable way is quite important, as we learned during our Human Practice activity. Here you can watch the video we created to make our project accessible to everyone and even non-scientists can get an impression on the research we performed during the this year´s iGEM season.