Team:Heidelberg/Human Practices

iGEM TEAM HEIDELBERG 2017

---

COLLAPSE NAVIGATION

---

• People
  • Team
  • Attributions
  • Sponsoring
  • For Judges
  • Giant Jamboree
• Project
  • Overview
  • Background & Design
  • PACE
  • PREDCEL
  • Results Summary
  • Software Validation
  • Organosilicons
  • Protein Interaction
  • Cytochrome Engineering
  • Optogenetics
  • CRISPR Cas9
  • InterLab Study
  • Achievements
• Parts
  • Overview
  • Basic Parts
  • Composite Parts
  • Improved Part
  • Parts Collection
  • Part List
  • RFC
• Modeling
  • Overview
  • Phage Titer
  • Mutagenesis Induction
  • Mutation Rate Estimation
  • Lagoon Contamination
  • Medium Consumption
  • Interactive Web Tools
• Software
  • AiGEM Overview
  • DeeProtein
  • GAIA
  • SafetyNet
  • MAWS 2.0
• Human Practices
  • Overview
  • Integrated Human Practices
  • Expert Interviews
  • Safety & Security
  • Public Engagement
  • Education
  • Collaborations
• Notebook
  • Overview
  • Methods
  • Materials
  • Notebook Database
• Toolbox Guide

Human Practices

Overview

Successful in vivo directed evolution by PREDCEL and PACE requires the thorough consideration of experimental parameters, e.g. phage propagation times, culture dilution rates and inducer/inhibitor concentrations. We employed extensive ODE-based and stochastic modeling to identify the most sensitive parameters and adapt our experiments accordingly. First, we calibrated our models using phage propagation experiments from our wet lab complemented with literature data. Simulations showed that the phage titer is highly sensitive to culture dilution rates. We simulated batch times and transfer volumes for PREDCEL and corresponding flow rates for PACE to determine optimized conditions for gene pool selection while avoiding phage washout. We also estimated phage titer monitoring intervals for cost/labor efficient QC as well as inducer/inhibitor concentrations required to express the required mutagenic polymerases. Finally, provide a web-based, fully interactive modeling platform, not only extensively employed by our wet lab, but highly informs future iGEM teams building on our work.

Human Practices - Silver Medal Criteria

Collaborations

Our evolutionary approach is mainly based on in vivo library generation by mutagenesis inducing plasmids. To ensure that these plasmids are reliably inducing mutations we performed an inter-lab study to validate the performance of the plasmids. Furthermore, we provided help to other iGEM teams (PCR First Aid Service), designed and performed a cloning for Freiburg and participated in several activities of other iGEM teams.

Collaborations

Experts

High impact projects with consequences affecting whole humanity should always be discussed with professionals with different scientific backgrounds. To meet this goal we talked to experts from theological, legal, safety and security, medical, agricultural, informatical, astrophysical and much more fields of interest. These talks helped us to evaluate possible consequences of our project and pathed the way for many subprojects.

{{{6}}}

Safety & Security

Although phages are volatile and challenging to work with, it is feasible to handle them safely provided good laboratory practice is applied with great awareness. To do so, we informed about environmental and personal protection measures and created special phage-spaces in the lab, to reduce contamination risk to a minimum.

Safety and Security

Education

Arousing interest of young people to shape the next generation of scientists is a core mission of our Human Practice activities. Wa gave an iGEM seminar at a local high school and hosted a school class from Berlin for a course on responsible genetic engineering in our lab. On top, we educated a high school student for a two weeks internship at our lab.

Education

Public Engagement

Colors illustrate diversity, opinions, hopes and concerns and we must consider the complex interplay of colors: Reach out, listen, talk and engage. In this spirit, we hosted an inspiring public lecture by Kevin Esvelt, went to TEDx Heidelberg and engaged with the people via our game “evolutionary wheel-of-fortune”. Finally, we created an animated video, explaining evolution and our project in a broadly comprehensible way.

Public Engagement

Responsibility

Developing and improving powerful and high impact techniques in the field of directed evolution requires responsible scientists. To encounter ethical concerns and ensure that our project is not endangering the environment or humanity we thoroughly confronted ourselves and experts with critical questions according our iGEM project.

Responsible Science

Human Practices - Gold Medal Criteria

Responsibility

Responsible science requires responsible scientists to respond to responsible responses responsibly.

Responsive Science

Collaborations

Although phages are volatile and challenging to work with, it is feasible to handle them safely provided good laboratory practice is applied with great awareness. To do so, we informed about environmental and personal protection measures and created special phage-spaces in the lab, to reduce contamination risk to a minimum.

Collaborative Activities

Expertise

Many experienced experts in their respective expert fields were expertifically interviewed during our Expert Interviews.

Expert Interviews