Difference between revisions of "Team:Paris Bettencourt/Improve"
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<div class=textbody> | <div class=textbody> | ||
<h1>Improve</h1> | <h1>Improve</h1> | ||
| − | <p> | + | <p> |
| + | Dronpa is a reversible photoswitchable fluorescent protein that is switched on by default “fluorescent” and is switched off when illuminated by cyan light (~500nm). Dronpa Fluorescence is recovered by shining violet light (~400nm). And has been used in a design that facilitates the optical control of protein activities | ||
| − | + | This part contains a device of two copies of Dronpa Fluorescent Protein that are codon optimized for E Coli with two BsaI cutting site in between to allow the insertion of various proteins to be tested . This coding sequence of the 2 dronpa domains has 2 mutations I4V and R149H in the first dronpa domain and an F78S mutation in the second domain that were obtained by error prone PCR. | |
| − | + | ||
| − | + | This mutant version of Dronpa has showed a better performance than the wild type in controling the activity of both TetR [fig1] and β-galactosidase[fig 3], the work flow of the β-galactosidase activity experiment is indicated in figure 2 </p> | |
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| + | <img id="fig2" src="https://static.igem.org/mediawiki/2017/6/61/Aya_figure_13.png" /> | ||
| + | </div> | ||
| + | <span class="image-span text-center"> | ||
| + | <b>Figure 2: </b> An overview of the experiment done to evaluate the activity of β-galactosidase-Dronpa fusion. | ||
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| + | </span> | ||
| + | |||
| + | <img id="fig3" src="https://static.igem.org/mediawiki/2017/0/01/Aya_figure_14.png" /> | ||
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| + | <span class="image-span text-center"> | ||
| + | <b>Figure 3: </b> X-Gal grayscale picture, testing the activity of β-galactosidase fusion with both wtDronpa and mutDronpa, indicating that β-galactosidase-mutDronpa fusion is more responsive to cyan light than the β-galactosidase-wtDronp. | ||
| + | |||
| + | </span> | ||
</div> | </div> | ||
Revision as of 02:56, 2 November 2017
Improve
Dronpa is a reversible photoswitchable fluorescent protein that is switched on by default “fluorescent” and is switched off when illuminated by cyan light (~500nm). Dronpa Fluorescence is recovered by shining violet light (~400nm). And has been used in a design that facilitates the optical control of protein activities This part contains a device of two copies of Dronpa Fluorescent Protein that are codon optimized for E Coli with two BsaI cutting site in between to allow the insertion of various proteins to be tested . This coding sequence of the 2 dronpa domains has 2 mutations I4V and R149H in the first dronpa domain and an F78S mutation in the second domain that were obtained by error prone PCR. This mutant version of Dronpa has showed a better performance than the wild type in controling the activity of both TetR [fig1] and β-galactosidase[fig 3], the work flow of the β-galactosidase activity experiment is indicated in figure 2
Figure 3: X-Gal grayscale picture, testing the activity of β-galactosidase fusion with both wtDronpa and mutDronpa, indicating that β-galactosidase-mutDronpa fusion is more responsive to cyan light than the β-galactosidase-wtDronp.
bettencourt.igem2017@gmail.com 
