Difference between revisions of "Team:William and Mary/Engagement"

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<center><h1>Outreach</h1></center>
 
 
 
 
 
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        <div ><a href ="https://2017.igem.org/Team:William_and_Mary/OutreachDatabase"><p style='font-size: 17px;'>Overview</p></a></div>
 
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        <div ><a href ="https://2017.igem.org/Team:William_and_Mary/OutreachDatabase"><p style='font-size: 17px;'>William & Mary</p></a></div>
 
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        <div ><a href ="https://2017.igem.org/Team:William_and_Mary/OutreachDatabase"><p style='font-size: 17px;'>iGEM Lab</p></a></div>
 
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        <div ><a href ="https://2017.igem.org/Team:William_and_Mary/OutreachDatabase"><p style='font-size: 17px;'>Community</p></a></div>
 
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        <div style='padding-right: 10px;'><a href ="https://2017.igem.org/Team:William_and_Mary/OutreachDatabase"><p style='font-size: 17px;'>Government</p></a></div>
 
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A fundamental goal of synthetic biology is to create a modular genetic basis for control over circuit behavior properties. While much progress has been made in achieving this objective for properties such as gene expression strength, where well-characterized ribosome binding sites (RBSs) can be conveniently swapped within a genetic part, there is much to be desired in altering gene expression speed. Currently, no such robust mechanism of speed control exists. We intend to provide a means to tune the speed of gene expression in transcriptional circuits, where a pre-characterized genetic part can be inserted into a gene to alter its expression in a predictable way.
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Introduction
 +
We constructed our outreach activities so that they worked together to meet an overall goal, which  was to help local middle school and high school students learn more about synthetic biology and to increase their future opportunities to be a part of this emerging field. To reach this goal we had two linked objectives. The first objective was to co-design strategies and educational materials to effectively reach this target audience.  The second, and arguably more important, objective was that the program be sustainable so that it can have a continuing impact on our community even beyond the end of the project. We started our outreach co-design process by consulting our community about their needs and what they would like to gain from a potential collaboration, as well as consulting education experts about the best way to get involved with teachers and students.
 +
To meet the second objective we found that we needed to expand our intended target audience to include educators. Including educators means that we can have a longer and larger impact through our local school system. But it also meant that we needed to adapt our educational materials to fit the context and incentives of the school system.
 +
Next, we did a wide array of outreach activities that helped address the goals of our community and our own goals:
 +
After consulting the School Education, we improved our synthetic biology Activities Booklet to make it more accessible to teachers. We then publicized it to educators in our teacher focus group and online. The booklet exists in a permanent form on our wiki, and will be accessible to everyone for years to come.
 +
We are working with educators to strengthen the network between W&M and the teachers in the school district, train them, and get feedback on the curriculum including how to make it fit into their overall STEM curriculum. Additionally, based on teacher feedback, we are in the process of forming a STEM outreach organization to continue our collaboration with teachers year-round.
 +
We also worked through civic organizations like the Girl Scouts, as well as a local STEM summer camp, that targeted this same age group.
 +
We worked with Virginia State Senator to improve networking with the school district and find out how to get our resources to the people who want them.
 +
We did general publicity of our research and the synthetic biology research at our college, since this field is still relatively new and not very well known.
 +
We created the Outreach Database, a compilation of the outreach projects done by gold medal teams in 2016 and 2015. This database organizes the project into categories and is a searchable tool for future iGEM teams to analyze past outreach and for teachers who want to find synthetic biology educational activities.
  
Based on multiple well-cited claims in literature, a strong relationship can be asserted between gene expression speed and the rate of protein degradation. Using a basic mathematical model of gene expression, one can derive that the speed of gene expression, defined as the time it takes for its protein product to reach half of its steady-state concentration, is a function of the protein’s degradation rate. This reveals that tuning protein degradation rate is essential to controlling gene expression, thus amenable to an approach involving protein degradation tags. Degradation tags are used endogenously to identify misfolded proteins, and different tags have unique protease-binding affinities which confer various degradation rates on the tagged proteins.
+
While some of our outreach to our community was short-term, we worked closely with our community partners to ensure that our impact does not end with this project. We are in the process of forming a recognized STEM outreach organization at our college that will consist of members of the 2017 team and other interested students.  
 
+
</div>
In 2008, the Sauer lab at MIT reported that Mycoplasma florum’s Lon protease system was orthogonal to the endogenous protein degradation machinery in E. coli. As of now, mf-Lon degradation tags exist only as isolated sequences on the BioBrick Registry. We intend to build a suite of BioBrick parts in the form of [mf-Lon tag] - [Stop Codon] - [Double Terminator] that can be swapped in to directly modulate protein degradation rate just as RBS and promoter sequences can be swapped to modify protein production. This would drastically increase the accessibility of mf-Lon tags and enable other teams to easily amplify their desired protein sequence by simply cloning it into our construct. Should the relationship between gene expression speed and protein degradation rate exist robustly, we will be providing the first modular genetic basis of speed control, fulfilling a core aspiration of synthetic biology to have every gene expression and circuit property accessible at the genetic level.</div>
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Revision as of 23:24, 31 October 2017



Introduction We constructed our outreach activities so that they worked together to meet an overall goal, which was to help local middle school and high school students learn more about synthetic biology and to increase their future opportunities to be a part of this emerging field. To reach this goal we had two linked objectives. The first objective was to co-design strategies and educational materials to effectively reach this target audience. The second, and arguably more important, objective was that the program be sustainable so that it can have a continuing impact on our community even beyond the end of the project. We started our outreach co-design process by consulting our community about their needs and what they would like to gain from a potential collaboration, as well as consulting education experts about the best way to get involved with teachers and students. To meet the second objective we found that we needed to expand our intended target audience to include educators. Including educators means that we can have a longer and larger impact through our local school system. But it also meant that we needed to adapt our educational materials to fit the context and incentives of the school system. Next, we did a wide array of outreach activities that helped address the goals of our community and our own goals: After consulting the School Education, we improved our synthetic biology Activities Booklet to make it more accessible to teachers. We then publicized it to educators in our teacher focus group and online. The booklet exists in a permanent form on our wiki, and will be accessible to everyone for years to come. We are working with educators to strengthen the network between W&M and the teachers in the school district, train them, and get feedback on the curriculum including how to make it fit into their overall STEM curriculum. Additionally, based on teacher feedback, we are in the process of forming a STEM outreach organization to continue our collaboration with teachers year-round. We also worked through civic organizations like the Girl Scouts, as well as a local STEM summer camp, that targeted this same age group. We worked with Virginia State Senator to improve networking with the school district and find out how to get our resources to the people who want them. We did general publicity of our research and the synthetic biology research at our college, since this field is still relatively new and not very well known. We created the Outreach Database, a compilation of the outreach projects done by gold medal teams in 2016 and 2015. This database organizes the project into categories and is a searchable tool for future iGEM teams to analyze past outreach and for teachers who want to find synthetic biology educational activities. While some of our outreach to our community was short-term, we worked closely with our community partners to ensure that our impact does not end with this project. We are in the process of forming a recognized STEM outreach organization at our college that will consist of members of the 2017 team and other interested students.


Database

Upcoming

Database

Upcoming

Database

Upcoming

Database

Upcoming