Team:William and Mary/Measurement

Enabling Future iGEM Teams

When Cameron and Collins demonstrated the functionality of protein degradation tags (pdt) and the Mesoplasma florum Lon (mf-Lon) protease in E. coli, they did their work exclusively using genomically integrated constructs. Since the majority of iGEM teams work mainly with plasmid constructs, we first wanted to confirm and characterize the parts using iGEM backbones. To do this we assembled constitutive and ATC inducible constructs carrying the red fluorescent protein mScarlet-I, tagged with each of our six different pdts, or left untagged as a control. Further, to ensure that our project will work with a variety of different proteins, we made identical constructs encoding for superfolder GFP (sfGFP) and performed preliminary characterization (Figure 2).

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