Difference between revisions of "Team:William and Mary/Results"

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Results
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Over the summer we successfully characterized each individual component of the Circuit Control Toolbox. Each of these components
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can be used either individually or in concert to orthogonally modify the output of transfer functions of an arbitrary genetic circuit.
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We also characterized many existing parts and created a predictive mathematical model of molecular titration. The entirety of the
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circuit toolbox as well as the parts used to characterized them has been submitted to the registry, on a unified backbone designed
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for ease of use with Gibson Assembly.
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<b>Achievements</b>
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<b>*</b> Characterized four orthogonal methods to control transfer functions.
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<p class='large' style="padding-left:100px; padding-bottom: 10px;">
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<b>1.</b> The ribozyme <a href= "https://2016.igem.org/Team:William_and_Mary/RiboJ">RiboJ</a> was used to insulate circuits and characterization from genetic context.
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<b>2.</b> A <a href= "https://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer"> synthetic enhancer</a> system was characterized, allowing for multistate response.
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<b>3.</b> The Community Library of <a href= "https://2016.igem.org/Team:William_and_Mary/RBS">RBSs</a> was characterized under a variety of conditions, using RiboJ to ensure that results were generalizable. These RBSs can be used to tune the amplitude of the transfer function of an arbitrary genetic circuit.
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<b>4.</b> <a href= "https://2016.igem.org/Team:William_and_Mary/Binding_Array">Molecular titration</a> was characterized using a repeat sequence array, which allows an arbitrary genetic circuit to shift it’s transfer function along the x axis.
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<b>*</b> Created a suite of parts that allows for the construction of variable length repeat LacO or TetO arrays.
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<b>*</b> Participated in the <a href= "https://2016.igem.org/Team:William_and_Mary/Interlab"> Interlab Measurement Project.</a>
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<p class='large'>
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<b>*</b> Created a <a href= "https://2016.igem.org/Team:William_and_Mary/Model">mathematical model</a> of molecular titration of arbitrary length repeat arrays.
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<b>*</b> Hosted several community events to raise awareness for synthetic biology
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</p>
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<p class='large' style="padding-left:70px; padding-bottom: 10px;">
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<b>></b> Hosted a Building with Biology Forum with the community to discuss the potential risks and benefits of synthetic biology tools such as CRISPR/Cas9.
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</p>
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<p class='large' style="padding-left:70px; padding-bottom: 10px;">
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<b>></b> Hosted a Building with Biology workshop with local children aged 6-13 to raise interest in the field of synthetic biology.
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</p>
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<p class='large' style="padding-left:70px; padding-bottom: 10px;">
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<b>></b> Hosted a synthetic biology event as part of the William and Mary summer program Higher Achievement in Middle Schoolers
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</p>
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<b>*</b> Created a website <a href= "learnsynbio.org">Learnsynbio.org</a> designed to provide an overview of of synthetic biology for High School students, which can be used either out of classroom, as a tie in to the AP curriculum, or as a supplemental unit.
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<b>*</b> <a href= "https://2016.igem.org/Team:William_and_Mary/Collaborations">Collaborated</a> with UPitt and Alverno to characterize our <a href= "https://2016.igem.org/Team:William_and_Mary/RBS">RBS</a> library in two different cell free systems.
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<b>*</b> In total submitted 118 new parts to the registry, many of which were characterized on several plasmid backbones.
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<h1>Results</h1>
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<p>Here you can describe the results of your project and your future plans. </p>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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<h5> Project Achievements </h5>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<h5>Inspiration</h5>
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<p>See how other teams presented their results.</p>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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Revision as of 19:39, 15 June 2017


...

Results

Over the summer we successfully characterized each individual component of the Circuit Control Toolbox. Each of these components can be used either individually or in concert to orthogonally modify the output of transfer functions of an arbitrary genetic circuit. We also characterized many existing parts and created a predictive mathematical model of molecular titration. The entirety of the circuit toolbox as well as the parts used to characterized them has been submitted to the registry, on a unified backbone designed for ease of use with Gibson Assembly.

Achievements

* Characterized four orthogonal methods to control transfer functions.

1. The ribozyme RiboJ was used to insulate circuits and characterization from genetic context.

2. A synthetic enhancer system was characterized, allowing for multistate response.

3. The Community Library of RBSs was characterized under a variety of conditions, using RiboJ to ensure that results were generalizable. These RBSs can be used to tune the amplitude of the transfer function of an arbitrary genetic circuit.

4. Molecular titration was characterized using a repeat sequence array, which allows an arbitrary genetic circuit to shift it’s transfer function along the x axis.

* Created a suite of parts that allows for the construction of variable length repeat LacO or TetO arrays.

* Participated in the Interlab Measurement Project.

* Created a mathematical model of molecular titration of arbitrary length repeat arrays.

* Hosted several community events to raise awareness for synthetic biology

> Hosted a Building with Biology Forum with the community to discuss the potential risks and benefits of synthetic biology tools such as CRISPR/Cas9.

> Hosted a Building with Biology workshop with local children aged 6-13 to raise interest in the field of synthetic biology.

> Hosted a synthetic biology event as part of the William and Mary summer program Higher Achievement in Middle Schoolers

* Created a website Learnsynbio.org designed to provide an overview of of synthetic biology for High School students, which can be used either out of classroom, as a tie in to the AP curriculum, or as a supplemental unit.

* Collaborated with UPitt and Alverno to characterize our RBS library in two different cell free systems.

* In total submitted 118 new parts to the registry, many of which were characterized on several plasmid backbones.