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| − | Results
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| − | <p class style='font-family: "candara"; font-size: 15px;'>
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| − | Over the summer we successfully characterized each individual component of the Circuit Control Toolbox. Each of these components
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| − | can be used either individually or in concert to orthogonally modify the output of transfer functions of an arbitrary genetic circuit.
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| − | We also characterized many existing parts and created a predictive mathematical model of molecular titration. The entirety of the
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| − | circuit toolbox as well as the parts used to characterized them has been submitted to the registry, on a unified backbone designed
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| − | for ease of use with Gibson Assembly.
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| − | </p>
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| − | <p class='large' style='padding-top:100px;'>
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| − | <b>Achievements</b>
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| − | <b>*</b> Characterized four orthogonal methods to control transfer functions.
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| − | </p>
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| − | <p class='large' style="padding-left:100px; padding-bottom: 10px;">
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| − | <b>1.</b> The ribozyme <a href= "https://2016.igem.org/Team:William_and_Mary/RiboJ">RiboJ</a> was used to insulate circuits and characterization from genetic context.
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| − | </p>
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| − | <p class='large' style="padding-left:100px; padding-bottom: 10px;">
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| − | <b>2.</b> A <a href= "https://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer"> synthetic enhancer</a> system was characterized, allowing for multistate response.
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| − | </p>
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| − | <p class='large' style="padding-left:100px; padding-bottom: 10px;">
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| − | <b>3.</b> The Community Library of <a href= "https://2016.igem.org/Team:William_and_Mary/RBS">RBSs</a> was characterized under a variety of conditions, using RiboJ to ensure that results were generalizable. These RBSs can be used to tune the amplitude of the transfer function of an arbitrary genetic circuit.
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| − | </p>
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| − | <b>4.</b> <a href= "https://2016.igem.org/Team:William_and_Mary/Binding_Array">Molecular titration</a> was characterized using a repeat sequence array, which allows an arbitrary genetic circuit to shift it’s transfer function along the x axis.
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| − | </p>
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| − | <p class='large'>
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| − | <b>*</b> Created a suite of parts that allows for the construction of variable length repeat LacO or TetO arrays.
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| − | </p>
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| − | <b>*</b> Participated in the <a href= "https://2016.igem.org/Team:William_and_Mary/Interlab"> Interlab Measurement Project.</a>
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| − | </p>
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| − | <p class='large'>
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| − | <b>*</b> Created a <a href= "https://2016.igem.org/Team:William_and_Mary/Model">mathematical model</a> of molecular titration of arbitrary length repeat arrays.
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| − | </p>
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| − | <p class='large'>
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| − | <b>*</b> Hosted several community events to raise awareness for synthetic biology
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| − | </p>
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| − | <p class='large' style="padding-left:70px; padding-bottom: 10px;">
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| − | <b>></b> Hosted a Building with Biology Forum with the community to discuss the potential risks and benefits of synthetic biology tools such as CRISPR/Cas9.
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| − | </p>
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| − | <p class='large' style="padding-left:70px; padding-bottom: 10px;">
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| − | <b>></b> Hosted a Building with Biology workshop with local children aged 6-13 to raise interest in the field of synthetic biology.
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| − | </p>
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| − | <p class='large' style="padding-left:70px; padding-bottom: 10px;">
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| − | <b>></b> Hosted a synthetic biology event as part of the William and Mary summer program Higher Achievement in Middle Schoolers
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| − | </p>
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| − | <p class='large'>
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| − | <b>*</b> Created a website <a href= "learnsynbio.org">Learnsynbio.org</a> designed to provide an overview of of synthetic biology for High School students, which can be used either out of classroom, as a tie in to the AP curriculum, or as a supplemental unit.
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| − | </p>
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| − | <p class='large'>
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| − | <b>*</b> <a href= "https://2016.igem.org/Team:William_and_Mary/Collaborations">Collaborated</a> with UPitt and Alverno to characterize our <a href= "https://2016.igem.org/Team:William_and_Mary/RBS">RBS</a> library in two different cell free systems.
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| − | </p>
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| − | <p class='large'>
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| − | <b>*</b> In total submitted 118 new parts to the registry, many of which were characterized on several plasmid backbones.
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